Preb prolactin regulatory element binding [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Prebprovided by MGI
Official Full Name: prolactin regulatory element bindingprovided by MGI
Primary source: MGI:MGI:1355326
See related: Ensembl:ENSMUSG00000045302 AllianceGenome:MGI:1355326
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: mSec12
Summary: Enables GTPase binding activity and guanyl-nucleotide exchange factor activity. Predicted to be involved in COPII vesicle coating; positive regulation of transcription by RNA polymerase II; and regulation of COPII vesicle coating. Predicted to be located in nucleus. Predicted to be active in endoplasmic reticulum exit site and endoplasmic reticulum membrane. Is expressed in several structures, including central nervous system; embryo mesenchyme; jaw; metanephros; and surface ectoderm. Orthologous to human PREB (prolactin regulatory element binding). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in duodenum adult (RPKM 35.0), large intestine adult (RPKM 34.4) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 5 B1; 5 16.9 cM

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (31109011..31117700, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (30951667..30960356, complement)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Glucose, insulin, and pyruvate tolerance were impaired in normal mice knocked down for Preb indicating Preb to be a negative regulator of liver gluconeogenic genes.
Title: Prolactin regulatory element-binding (PREB) protein regulates hepatic glucose homeostasis.
PREB may contribute to the regulation of ApN gene transcription, in response to cAMP activation in adipocytes.
Title: Prolactin regulatory element-binding protein involved in cAMP-mediated suppression of adiponectin gene.
These data showed that in the adrenal gland, PREB regulates the transcription of the CYP11B1 gene via cAMP.
Title: The prolactin regulatory element-binding regulates of the 11beta-hydroxylase gene.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Preb (e-PCR), detects polymorphism
- UniSTS:164696

NoName (e-PCR)
- UniSTS:235155

RH127221 (e-PCR)
- UniSTS:207767

Abhd1 (e-PCR), detects polymorphism
- UniSTS:265208

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables GTPase binding	IPI Inferred from Physical Interaction more info	PubMed
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info
enables guanyl-nucleotide exchange factor activity	IDA Inferred from Direct Assay more info	PubMed
enables guanyl-nucleotide exchange factor activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in COPII vesicle coating	ISO Inferred from Sequence Orthology more info
involved_in COPII vesicle coating	ISS Inferred from Sequence or Structural Similarity more info
involved_in endoplasmic reticulum to Golgi vesicle-mediated transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in endoplasmic reticulum to Golgi vesicle-mediated transport	ISO Inferred from Sequence Orthology more info
involved_in endoplasmic reticulum to Golgi vesicle-mediated transport	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in protein transport	IEA Inferred from Electronic Annotation more info
involved_in regulation of COPII vesicle coating	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of COPII vesicle coating	IEA Inferred from Electronic Annotation more info
involved_in regulation of COPII vesicle coating	ISO Inferred from Sequence Orthology more info
involved_in regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in endoplasmic reticulum exit site	ISO Inferred from Sequence Orthology more info
is_active_in endoplasmic reticulum exit site	ISS Inferred from Sequence or Structural Similarity more info
is_active_in endoplasmic reticulum membrane	IBA Inferred from Biological aspect of Ancestor more info
is_active_in endoplasmic reticulum membrane	ISO Inferred from Sequence Orthology more info
is_active_in endoplasmic reticulum membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in nucleus	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: guanine nucleotide-exchange factor SEC12; prolactin regulatory element-binding protein

Names: mammalian guanine nucleotide exchange factor Sec12; mammalian guanine nucleotide exchange factor mSec12

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001294302.1 → NP_001281231.1 guanine nucleotide-exchange factor SEC12 isoform 2

See identical proteins and their annotated locations for NP_001281231.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks an alternate exon in the 3' coding region, resulting in a frameshift and an early stop codon, compared to variant 1. It encodes isoform 2, which is shorter and has a distinct C-terminus, compared to isoform 1.

Source sequence(s)

AK013330, AK029838, AK151288, BP765513, CJ145010

Consensus CDS

CCDS80244.1

UniProtKB/TrEMBL

D3Z3S1

Related

ENSMUSP00000144263.2, ENSMUST00000202567.2

Conserved Domains (3) summary

COG2319
Location:140 → 327

WD40; WD40 repeat [General function prediction only]

sd00039
Location:160 → 194

7WD40; WD40 repeat [structural motif]

cl02567
Location:159 → 327

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...
NM_001422185.1 → NP_001409114.1 guanine nucleotide-exchange factor SEC12 isoform 3

Status: VALIDATED

Source sequence(s)

AC109606
NM_016703.3 → NP_057912.2 guanine nucleotide-exchange factor SEC12 isoform 1

See identical proteins and their annotated locations for NP_057912.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encode the longer isoform (1).

Source sequence(s)

AK013330, AK029838, AK151288, BP765513, CJ145010

Consensus CDS

CCDS39051.1

UniProtKB/Swiss-Prot

Q9QZ99, Q9WUQ2

UniProtKB/TrEMBL

Q3UAP1

Related

ENSMUSP00000074387.6, ENSMUST00000074840.12

Conserved Domains (2) summary

sd00039
Location:160 → 194

7WD40; WD40 repeat [structural motif]

cl29593
Location:159 → 353

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...

RNA

NR_185490.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC109606
NR_185491.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC109606

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000071.7 Reference GRCm39 C57BL/6J

Range

31109011..31117700 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006504015.2 → XP_006504078.1 prolactin regulatory element-binding protein isoform X3

Conserved Domains (2) summary

sd00039
Location:153 → 193

7WD40; WD40 repeat [structural motif]

cl29593
Location:159 → 231

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...
XM_036165264.1 → XP_036021157.1 prolactin regulatory element-binding protein isoform X1

Conserved Domains (2) summary

sd00039
Location:153 → 193

7WD40; WD40 repeat [structural motif]

cl29593
Location:159 → 231

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...
XM_006504017.4 → XP_006504080.1 prolactin regulatory element-binding protein isoform X4

Conserved Domains (2) summary

sd00039
Location:153 → 193

7WD40; WD40 repeat [structural motif]

cl29593
Location:159 → 231

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...