Lrrc32 leucine rich repeat containing 32 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Lrrc32provided by MGI
Official Full Name: leucine rich repeat containing 32provided by MGI
Primary source: MGI:MGI:93882
See related: Ensembl:ENSMUSG00000090958 AllianceGenome:MGI:93882
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Garp; EG434215; D11S833Eh; D7H11S833E
Summary: Enables transforming growth factor beta binding activity. Involved in establishment of protein localization to extracellular region and secondary palate development. Acts upstream of or within negative regulation of activated T cell proliferation; negative regulation of cytokine production; and positive regulation of gene expression. Predicted to be located in cell surface and nucleoplasm. Predicted to be active in external side of plasma membrane; extracellular matrix; and extracellular space. Is expressed in several structures, including genitourinary system; jaw; lung; sensory organ; and skeletal muscle. Orthologous to human LRRC32 (leucine rich repeat containing 32). [provided by Alliance of Genome Resources, Oct 2024]
Expression: Ubiquitous expression in lung adult (RPKM 7.0), subcutaneous fat pad adult (RPKM 6.7) and 26 other tissues See more
Orthologs: human all
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Genomic context

Location:: 7 E1; 7 53.86 cM

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	7	NC_000073.7 (98138515..98151038)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	7	NC_000073.6 (98489305..98501831)

Chromosome 7 - NC_000073.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Thrombin contributes to cancer immune evasion via proteolysis of platelet-bound GARP to activate LTGF-beta.
Title: Thrombin contributes to cancer immune evasion via proteolysis of platelet-bound GARP to activate LTGF-β.
Study demonstrated that B cells are required for the induction of oral tolerance of T cell-dependent antigens via GARP and revealed for the first time that cell surface GARP-TGF-beta is an important checkpoint for regulating B cell peripheral tolerance, highlighting a mechanism of autoimmune disease pathogenesis.
Title: B lymphocytes confer immune tolerance via cell surface GARP-TGF-β complex.
Data (including data from studies using knockout mice) suggest that Garp/Lrrc32 is involved in up-regulation of Tgfb3 and is essential for normal embryogenesis of palate; knockout of Garp causes postnatal lethality, cleft palate, and decreased apoptosis and Smad2 phosphorylation in medial edge epithelial cells of palatal shelf of embryos. (Tgfb3 = transforming growth factor beta 3; Smad2 = MAD homolog protein 2)
Title: Glycoprotein A repetitions predominant (GARP) positively regulates transforming growth factor (TGF) β3 and is essential for mouse palatogenesis.
These results demonstrate the unexpected presence of GARP on Hepatic stellate cells and its significance in regard to the ability of Hepatic stellate cells to activate latent TGF-beta1 and thereby inhibit T cells.
Title: Hepatic Stellate Cells Inhibit T Cells through Active TGF-β1 from a Cell Surface-Bound Latent TGF-β1/GARP Complex.
GARP expression on MSCs contributed to their ability to inhibit T-cell responses in vitro.
Title: Mesenchymal stromal cells express GARP/LRRC32 on their surface: effects on their biology and immunomodulatory capacity.
We conclude that a missense mutation in VPS54, an essential component of the Golgi-associated retrograde protein complex, not only prevents the formation of an acrosome but also initiates a cascade of metabolic abnormalities and a stress reaction.
Title: Pathoproteomics of testicular tissue deficient in the GARP component VPS54: the wobbler mouse model of globozoospermia.
These findings suggest a role for GARP in natural and induced Treg development through activation of bound latent TGF-beta and signaling, which negatively regulates GARP expression on Tregs.
Title: GARP-TGF-β complexes negatively regulate regulatory T cell development and maintenance of peripheral CD4+ T cells in vivo.
GARP is a regulatory T cell specific cell surface molecule that mediates suppressive signals and induces Foxp3 expression
Title: Identification of a regulatory T cell specific cell surface molecule that mediates suppressive signals and induces Foxp3 expression.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (4)
Targeted (4) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables receptor ligand inhibitor activity	IEA Inferred from Electronic Annotation more info
enables receptor ligand inhibitor activity	ISO Inferred from Sequence Orthology more info
enables transforming growth factor beta binding	IBA Inferred from Biological aspect of Ancestor more info
enables transforming growth factor beta binding	IPI Inferred from Physical Interaction more info	PubMed
enables transforming growth factor beta binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in establishment of protein localization to extracellular region	IDA Inferred from Direct Assay more info	PubMed
involved_in establishment of protein localization to extracellular region	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of activated T cell proliferation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of cytokine production	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of gene expression	IDA Inferred from Direct Assay more info	PubMed
involved_in secondary palate development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transforming growth factor beta receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in transforming growth factor beta receptor signaling pathway	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
located_in cell surface	ISO Inferred from Sequence Orthology more info
located_in cell surface	ISS Inferred from Sequence or Structural Similarity more info
is_active_in external side of plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
is_active_in extracellular matrix	IBA Inferred from Biological aspect of Ancestor more info
is_active_in extracellular space	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: transforming growth factor beta activator LRRC32

Names: garpin; glycoprotein A repetitions predominant; leucine-rich repeat-containing protein 32

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001113379.2 → NP_001106850.1 transforming growth factor beta activator LRRC32 precursor

See identical proteins and their annotated locations for NP_001106850.1

Status: VALIDATED

Source sequence(s)

AC115069

Consensus CDS

CCDS52319.1

UniProtKB/Swiss-Prot

A0A0U1RQ93, G3XA59

Related

ENSMUSP00000145859.2, ENSMUST00000205956.2

Conserved Domains (4) summary

PLN00113
Location:123 → 562

PLN00113; leucine-rich repeat receptor-like protein kinase; Provisional

sd00031
Location:564 → 576

LRR_1; leucine-rich repeat [structural motif]

sd00033
Location:55 → 75

LRR_RI; leucine-rich repeat [structural motif]

cl39015
Location:37 → 278

LRR_RI; Leucine-rich repeats (LRRs), ribonuclease inhibitor (RI)-like subfamily. LRRs are 20-29 residue sequence motifs present in many proteins that participate in protein-protein interactions and have different functions and cellular locations. LRRs correspond ...

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000073.7 Reference GRCm39 C57BL/6J

Range

98138515..98151038

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006507979.4 → XP_006508042.1 transforming growth factor beta activator LRRC32 isoform X1

See identical proteins and their annotated locations for XP_006508042.1

UniProtKB/Swiss-Prot

A0A0U1RQ93, G3XA59

Related

ENSMUSP00000133205.2, ENSMUST00000165205.2

Conserved Domains (4) summary

PLN00113
Location:123 → 562

PLN00113; leucine-rich repeat receptor-like protein kinase; Provisional

sd00031
Location:564 → 576

LRR_1; leucine-rich repeat [structural motif]

sd00033
Location:55 → 75

LRR_RI; leucine-rich repeat [structural motif]

cl39015
Location:37 → 278

LRR_RI; Leucine-rich repeats (LRRs), ribonuclease inhibitor (RI)-like subfamily. LRRs are 20-29 residue sequence motifs present in many proteins that participate in protein-protein interactions and have different functions and cellular locations. LRRs correspond ...
XM_006507978.4 → XP_006508041.1 transforming growth factor beta activator LRRC32 isoform X1

See identical proteins and their annotated locations for XP_006508041.1

UniProtKB/Swiss-Prot

A0A0U1RQ93, G3XA59

Conserved Domains (4) summary

PLN00113
Location:123 → 562

PLN00113; leucine-rich repeat receptor-like protein kinase; Provisional

sd00031
Location:564 → 576

LRR_1; leucine-rich repeat [structural motif]

sd00033
Location:55 → 75

LRR_RI; leucine-rich repeat [structural motif]

cl39015
Location:37 → 278

LRR_RI; Leucine-rich repeats (LRRs), ribonuclease inhibitor (RI)-like subfamily. LRRs are 20-29 residue sequence motifs present in many proteins that participate in protein-protein interactions and have different functions and cellular locations. LRRs correspond ...