Pias1 protein inhibitor of activated STAT, 1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Pias1provided by RGD
Official Full Name: protein inhibitor of activated STAT, 1provided by RGD
Primary source: RGD:1307843
See related: EnsemblRapid:ENSRNOG00000034272 AllianceGenome:RGD:1307843
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables SUMO transferase activity and protein domain specific binding activity. Involved in several processes, including G1/S transition of mitotic cell cycle; positive regulation of macromolecule metabolic process; and visual learning. Predicted to be located in PML body; chromatin; and nuclear periphery. Predicted to be active in glutamatergic synapse; postsynaptic cytosol; and presynaptic cytosol. Biomarker of focal segmental glomerulosclerosis. Orthologous to human PIAS1 (protein inhibitor of activated STAT 1). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Testes (RPKM 162.4), Thymus (RPKM 154.5) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 8q24

Exon count:: 18

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	8	NC_086026.1 (72233566..72347085, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	8	NC_051343.1 (63338150..63451670, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	8	NC_005107.4 (67771720..67869015, complement)

Chromosome 8 - NC_086026.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

This study provide the first evidence that CREB could be SUMO-modified by the E3 ligase PIAS1 and that CREB SUMOylation plays an important role in spatial memory formation
Title: CREB SUMOylation by the E3 ligase PIAS1 enhances spatial memory.
PIAS1 may function as an immediate early gene in an activity-dependent manner: its expression is rapidly induced upon NMDA receptor activation in rat hippocampus.
Title: NMDA receptor signaling mediates the expression of protein inhibitor of activated STAT1 (PIAS1) in rat hippocampus.
SUMO E3 ligases are expressed in the retina and regulate SUMOylation of the metabotropic glutamate receptor 8b.
Title: SUMO E3 ligases are expressed in the retina and regulate SUMOylation of the metabotropic glutamate receptor 8b.
PIAS1 could augment anti-inflammatory activity by inhibiting STAT1, thus attenuating the severity of SAP associated with acute lung injury
Title: Upregulation of PIAS1 protects against sodium taurocholate-induced severe acute pancreatitis associated with acute lung injury.
The results indicated that in the cerulein-stimulated PIASI silencing cells, the activity of p38 MAP kinase was enhanced, while the levels of inflammatory mediators were markedly higher than those of other cerulein-stimulated cells.
Title: SiRNA-mediated PIAS1 silencing promotes inflammatory response and leads to injury of cerulein-stimulated pancreatic acinar cells via regulation of the P38MAPK signaling pathway.
Immunohistochemistry showed that Ubc9 and PIAS1 are markedly expressed in rat adrenal glomerulosa cells
Title: Ubc9 and Protein Inhibitor of Activated STAT 1 Activate Chicken Ovalbumin Upstream Promoter-Transcription Factor I-mediated Human CYP11B2 Gene Transcription.
Pias1 binds to and sumoylates metabotropic glutamate receptor 8
Title: Pias1 interaction and sumoylation of metabotropic glutamate receptor 8.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AI179763 (e-PCR)
- UniSTS:250655

BE097511 (e-PCR)
- UniSTS:244683

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables DNA-binding transcription factor binding	ISO Inferred from Sequence Orthology more info
enables SUMO ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables SUMO ligase activity	ISO Inferred from Sequence Orthology more info
enables SUMO transferase activity	EXP Inferred from Experiment more info	PubMed
enables SUMO transferase activity	ISO Inferred from Sequence Orthology more info
enables enzyme binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	IDA Inferred from Direct Assay more info	PubMed
enables protein domain specific binding	IPI Inferred from Physical Interaction more info	PubMed
enables transcription cis-regulatory region binding	ISO Inferred from Sequence Orthology more info
enables transcription coregulator activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase binding	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA damage response	ISO Inferred from Sequence Orthology more info
involved_in G1/S transition of mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in fat cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of proteasomal ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein localization to cell periphery	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein sumoylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of protein sumoylation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of smooth muscle cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein sumoylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein sumoylation	IEA Inferred from Electronic Annotation more info
involved_in protein sumoylation	ISO Inferred from Sequence Orthology more info
involved_in protein-DNA complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell population proliferation	ISO Inferred from Sequence Orthology more info
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in spermatogenesis	IEP Inferred from Expression Pattern more info	PubMed
involved_in visual learning	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in PML body	ISO Inferred from Sequence Orthology more info
located_in chromatin	IBA Inferred from Biological aspect of Ancestor more info
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
located_in nuclear periphery	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	TAS Traceable Author Statement more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
is_active_in postsynaptic cytosol	ISO Inferred from Sequence Orthology more info
is_active_in presynaptic cytosol	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: E3 SUMO-protein ligase PIAS1

NP_001100299.3

EC 2.3.2.27

XP_038937035.1

EC 2.3.2.27

XP_038937037.1

EC 2.3.2.27

XP_038937038.1

EC 2.3.2.27

XP_038937039.1

EC 2.3.2.27

XP_063121223.1

EC 2.3.2.27

XP_063121224.1

EC 2.3.2.27

XP_063121225.1

EC 2.3.2.27

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001106829.3 → NP_001100299.3 E3 SUMO-protein ligase PIAS1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000008

UniProtKB/TrEMBL

A0A8I6AIY4, A6J591, A9UK05

Related

ENSRNOP00000093422.1, ENSRNOT00000100439.2

Conserved Domains (4) summary

smart00513
Location:11 → 45

SAP; Putative DNA-binding (bihelical) motif predicted to be involved in chromosomal organisation

COG5222
Location:313 → 428

COG5222; Uncharacterized conserved protein, contains RING Zn-finger [General function prediction only]

cd16790
Location:332 → 379

SP-RING_PIAS; SP-RING finger found in protein inhibitor of activated signal transducer and activator of transcription (PIAS) proteins

pfam14324
Location:137 → 285

PINIT; PINIT domain

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086026.1 Reference GRCr8

Range

72233566..72347085 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039081109.2 → XP_038937037.1 E3 SUMO-protein ligase PIAS1 isoform X1

UniProtKB/TrEMBL

A9UK05

Conserved Domains (4) summary

smart00513
Location:2 → 36

SAP; Putative DNA-binding (bihelical) motif predicted to be involved in chromosomal organisation

COG5222
Location:304 → 419

COG5222; Uncharacterized conserved protein, contains RING Zn-finger [General function prediction only]

cd16790
Location:323 → 370

SP-RING_PIAS; SP-RING finger found in protein inhibitor of activated signal transducer and activator of transcription (PIAS) proteins

pfam14324
Location:128 → 276

PINIT; PINIT domain
XM_039081110.2 → XP_038937038.1 E3 SUMO-protein ligase PIAS1 isoform X2

UniProtKB/TrEMBL

A0A8I5Y680

Related

ENSRNOP00000077365.1, ENSRNOT00000097366.2

Conserved Domains (4) summary

smart00513
Location:11 → 45

SAP; Putative DNA-binding (bihelical) motif predicted to be involved in chromosomal organisation

COG5222
Location:313 → 428

COG5222; Uncharacterized conserved protein, contains RING Zn-finger [General function prediction only]

cd16790
Location:332 → 379

SP-RING_PIAS; SP-RING finger found in protein inhibitor of activated signal transducer and activator of transcription (PIAS) proteins

pfam14324
Location:137 → 285

PINIT; PINIT domain
XM_039081111.2 → XP_038937039.1 E3 SUMO-protein ligase PIAS1 isoform X1

UniProtKB/TrEMBL

A9UK05

Conserved Domains (4) summary

smart00513
Location:2 → 36

SAP; Putative DNA-binding (bihelical) motif predicted to be involved in chromosomal organisation

COG5222
Location:304 → 419

COG5222; Uncharacterized conserved protein, contains RING Zn-finger [General function prediction only]

cd16790
Location:323 → 370

SP-RING_PIAS; SP-RING finger found in protein inhibitor of activated signal transducer and activator of transcription (PIAS) proteins

pfam14324
Location:128 → 276

PINIT; PINIT domain
XM_063265155.1 → XP_063121225.1 E3 SUMO-protein ligase PIAS1 isoform X3
XM_063265154.1 → XP_063121224.1 E3 SUMO-protein ligase PIAS1 isoform X3
XM_063265153.1 → XP_063121223.1 E3 SUMO-protein ligase PIAS1 isoform X1
XM_039081107.2 → XP_038937035.1 E3 SUMO-protein ligase PIAS1 isoform X1

UniProtKB/TrEMBL

A9UK05

Conserved Domains (4) summary

smart00513
Location:2 → 36

SAP; Putative DNA-binding (bihelical) motif predicted to be involved in chromosomal organisation

COG5222
Location:304 → 419

COG5222; Uncharacterized conserved protein, contains RING Zn-finger [General function prediction only]

cd16790
Location:323 → 370

SP-RING_PIAS; SP-RING finger found in protein inhibitor of activated signal transducer and activator of transcription (PIAS) proteins

pfam14324
Location:128 → 276

PINIT; PINIT domain