Add3 adducin 3 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Add3provided by RGD
Official Full Name: adducin 3provided by RGD
Primary source: RGD:2043
See related: EnsemblRapid:ENSRNOG00000012820 AllianceGenome:RGD:2043
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables protein kinase C binding activity. Involved in positive regulation of cytoskeleton organization; positive regulation of vasoconstriction; and response to xenobiotic stimulus. Predicted to be located in several cellular components, including brush border; cell-cell junction; and condensed nuclear chromosome. Predicted to be active in cytoskeleton; plasma membrane; and postsynaptic density. Used to study hypertension and proteinuria. Human ortholog(s) of this gene implicated in spastic quadriplegic cerebral palsy 3. Orthologous to human ADD3 (adducin 3). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Kidney (RPKM 341.8), Lung (RPKM 213.6) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 1q55

Exon count:: 21

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	1	NC_086019.1 (262152722..262260504)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	1	NC_051336.1 (252147341..252255126)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	1	NC_005100.4 (273854195..273961982)

Chromosome 1 - NC_086019.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Reduced ADD3 function in FHH rats alters baseline podocyte pathophysiology by rearrangement of the actin cytoskeleton at the onset of proteinuria in young animals.
Title: Role of γ-adducin in actin cytoskeleton rearrangements in podocyte pathophysiology.
A mutation in ADD3 that alters ACTIN binding causes renal vascular dysfunction and promotes the susceptibility to kidney disease.
Title: A Mutation in γ-Adducin Impairs Autoregulation of Renal Blood Flow and Promotes the Development of Kidney Disease.
Mutation of Add3 may play a causal role in the impaired myogenic response and blood flow autoregulation in the renal and cerebral circulation.
Title: Knockdown of Add3 impairs the myogenic response of renal afferent arterioles and middle cerebral arteries.
Knockout of gamma-Adducin Promotes N(G)-Nitro-L-Arginine-Methyl-Ester-Induced Hypertensive Renal Injury.
Title: Knockout of γ-Adducin Promotes N(G)-Nitro-L-Arginine-Methyl-Ester-Induced Hypertensive Renal Injury.
Role of gamma-adducin in actin cytoskeleton rearrangements in podocyte pathophysiology.
Title: Role of γ-adducin in actin cytoskeleton rearrangements in podocyte pathophysiology.
A Mutation in gamma-Adducin Impairs Autoregulation of Renal Blood Flow and Promotes the Development of Kidney Disease.
Title: A Mutation in γ-Adducin Impairs Autoregulation of Renal Blood Flow and Promotes the Development of Kidney Disease.
Add3 may play a causal role in the impaired myogenic response and autoregulation in the renal and cerebral circulation in hypertensive rats.
Title: Knockdown of Add3 impairs the myogenic response of renal afferent arterioles and middle cerebral arteries.
Decrease in the expression of brain gamma-adducin in spontaneously hypertensive rats compared to its normotensive control.
Title: Hypertension-linked decrease in the expression of brain gamma-adducin.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH127841 (e-PCR)
- UniSTS:211151

D1Got253 (e-PCR)
- UniSTS:113108

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables actin filament binding	IBA Inferred from Biological aspect of Ancestor more info
enables calmodulin binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase C binding	IDA Inferred from Direct Assay more info	PubMed
enables structural constituent of cytoskeleton	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in barbed-end actin filament capping	IBA Inferred from Biological aspect of Ancestor more info
involved_in cytoskeleton organization	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of vasoconstriction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to xenobiotic stimulus	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in brush border	ISO Inferred from Sequence Orthology more info
located_in cell cortex	ISO Inferred from Sequence Orthology more info
located_in cell-cell junction	ISO Inferred from Sequence Orthology more info
located_in condensed nuclear chromosome	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in membrane	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in postsynaptic density	IBA Inferred from Biological aspect of Ancestor more info
located_in postsynaptic density	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: gamma-adducin

Names: adducin 3 (gamma); adducin-like protein 70; potein kinase C binding protein 35H; protein kinase C-binding protein 35H

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001164103.1 → NP_001157575.1 gamma-adducin isoform 1

See identical proteins and their annotated locations for NP_001157575.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AA899625, AW141606, CB725075, CB757924, CF111029, CK596161, FM038946, FM059497, FM072500, U35775

UniProtKB/Swiss-Prot

D3ZCH7, G3V9D7, Q62847

Conserved Domains (1) summary

cl00214
Location:137 → 385

Aldolase_II; Class II Aldolase and Adducin head (N-terminal) domain. Aldolases are ubiquitous enzymes catalyzing central steps of carbohydrate metabolism. Based on enzymatic mechanisms, this superfamily has been divided into two distinct classes (Class I and II). ...
NM_031552.2 → NP_113740.2 gamma-adducin isoform 2

See identical proteins and their annotated locations for NP_113740.2

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment near the C-terminus, compared to isoform 1.

Source sequence(s)

AA899625, AW141606, CB757924, CF111029, CK596161, CO402732, FM038946, FM059497, FM072500, U35775

UniProtKB/Swiss-Prot

Q62847

UniProtKB/TrEMBL

D3ZCH7

Conserved Domains (1) summary

cl00214
Location:137 → 385

Aldolase_II; Class II Aldolase and Adducin head (N-terminal) domain. Aldolases are ubiquitous enzymes catalyzing central steps of carbohydrate metabolism. Based on enzymatic mechanisms, this superfamily has been divided into two distinct classes (Class I and II). ...

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086019.1 Reference GRCr8

Range

262152722..262260504

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006231600.5 → XP_006231662.1 gamma-adducin isoform X2

See identical proteins and their annotated locations for XP_006231662.1

UniProtKB/Swiss-Prot

D3ZCH7, G3V9D7, Q62847

Conserved Domains (1) summary

cl00214
Location:137 → 385

Aldolase_II; Class II Aldolase and Adducin head (N-terminal) domain. Aldolases are ubiquitous enzymes catalyzing central steps of carbohydrate metabolism. Based on enzymatic mechanisms, this superfamily has been divided into two distinct classes (Class I and II). ...
XM_039101448.2 → XP_038957376.1 gamma-adducin isoform X2

UniProtKB/Swiss-Prot

D3ZCH7, G3V9D7, Q62847

Conserved Domains (1) summary

cl00214
Location:137 → 385

Aldolase_II; Class II Aldolase and Adducin head (N-terminal) domain. Aldolases are ubiquitous enzymes catalyzing central steps of carbohydrate metabolism. Based on enzymatic mechanisms, this superfamily has been divided into two distinct classes (Class I and II). ...
XM_063281425.1 → XP_063137495.1 gamma-adducin isoform X4
XM_008760510.4 → XP_008758732.1 gamma-adducin isoform X2

See identical proteins and their annotated locations for XP_008758732.1

UniProtKB/Swiss-Prot

D3ZCH7, G3V9D7, Q62847

Related

ENSRNOP00000110239.1, ENSRNOT00000132974.1

Conserved Domains (1) summary

cl00214
Location:137 → 385

Aldolase_II; Class II Aldolase and Adducin head (N-terminal) domain. Aldolases are ubiquitous enzymes catalyzing central steps of carbohydrate metabolism. Based on enzymatic mechanisms, this superfamily has been divided into two distinct classes (Class I and II). ...
XM_006231599.5 → XP_006231661.1 gamma-adducin isoform X2

See identical proteins and their annotated locations for XP_006231661.1

UniProtKB/Swiss-Prot

D3ZCH7, G3V9D7, Q62847

Conserved Domains (1) summary

cl00214
Location:137 → 385

Aldolase_II; Class II Aldolase and Adducin head (N-terminal) domain. Aldolases are ubiquitous enzymes catalyzing central steps of carbohydrate metabolism. Based on enzymatic mechanisms, this superfamily has been divided into two distinct classes (Class I and II). ...
XM_063281414.1 → XP_063137484.1 gamma-adducin isoform X4

Related

ENSRNOP00000112493.1, ENSRNOT00000171420.1
XM_006231601.5 → XP_006231663.1 gamma-adducin isoform X2

See identical proteins and their annotated locations for XP_006231663.1

UniProtKB/Swiss-Prot

D3ZCH7, G3V9D7, Q62847

Related

ENSRNOP00000109495.1, ENSRNOT00000128366.1

Conserved Domains (1) summary

cl00214
Location:137 → 385

Aldolase_II; Class II Aldolase and Adducin head (N-terminal) domain. Aldolases are ubiquitous enzymes catalyzing central steps of carbohydrate metabolism. Based on enzymatic mechanisms, this superfamily has been divided into two distinct classes (Class I and II). ...
XM_063281396.1 → XP_063137466.1 gamma-adducin isoform X2

UniProtKB/Swiss-Prot

D3ZCH7, G3V9D7, Q62847

Related

ENSRNOP00000017600.6, ENSRNOT00000017600.8
XM_063281404.1 → XP_063137474.1 gamma-adducin isoform X3
XM_063281377.1 → XP_063137447.1 gamma-adducin isoform X1

Related

ENSRNOP00000043399.4, ENSRNOT00000044827.8