Napepld N-acyl phosphatidylethanolamine phospholipase D [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Napepldprovided by MGI
Official Full Name: N-acyl phosphatidylethanolamine phospholipase Dprovided by MGI
Primary source: MGI:MGI:2140885
See related: Ensembl:ENSMUSG00000044968 AllianceGenome:MGI:2140885
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Mbldc1; NAPE-PLD; A530089G06
Summary: Enables N-acylphosphatidylethanolamine-specific phospholipase D activity. Involved in several processes, including N-acylphosphatidylethanolamine metabolic process; host-mediated regulation of intestinal microbiota composition; and positive regulation of brown fat cell differentiation. Acts upstream of or within N-acylethanolamine metabolic process. Is active in hippocampal mossy fiber to CA3 synapse; smooth endoplasmic reticulum membrane; and synaptic membrane. Orthologous to human NAPEPLD (N-acyl phosphatidylethanolamine phospholipase D). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Broad expression in adrenal adult (RPKM 7.3), testis adult (RPKM 5.6) and 27 other tissues See more
Orthologs: human all
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Genomic context

Location:: 5 A3; 5 9.97 cM

Exon count:: 7

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (21867899..21906620, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (21662901..21701622, complement)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

NAPE-specific phospholipase D regulates LRRK2 association with neuronal membranes.
Title: NAPE-specific phospholipase D regulates LRRK2 association with neuronal membranes.
Hepatic NAPE-PLD Is a Key Regulator of Liver Lipid Metabolism.
Title: Hepatic NAPE-PLD Is a Key Regulator of Liver Lipid Metabolism.
Intestinal NAPE-PLD contributes to short-term regulation of food intake via gut-to-brain axis.
Title: Intestinal NAPE-PLD contributes to short-term regulation of food intake via gut-to-brain axis.
Symmetrically substituted dichlorophenes inhibit N-acyl-phosphatidylethanolamine phospholipase D.
Title: Symmetrically substituted dichlorophenes inhibit N-acyl-phosphatidylethanolamine phospholipase D.
A protective role for N-acylphosphatidylethanolamine phospholipase D in 6-OHDA-induced neurodegeneration.
Title: A protective role for N-acylphosphatidylethanolamine phospholipase D in 6-OHDA-induced neurodegeneration.
Data indicate that N-acyl phosphatidylethanolamine phospholipase D (NAPE-PLD) knockout mice showed a broad range of lipids that were differentially affected by lipid species and brain region.
Title: Lipidomics profile of a NAPE-PLD KO mouse provides evidence of a broader role of this enzyme in lipid metabolism in the brain.
Adipose tissue NAPE-PLD controls fat mass development by altering the browning process and gut microbiota.
Title: Adipose tissue NAPE-PLD controls fat mass development by altering the browning process and gut microbiota.
Authors demonstrated that phospholipase D and diacylglycerol lipase were required for providing arachidonic acid for prostaglandin E(2) biosynthesis.
Title: Francisella tularensis LVS induction of prostaglandin biosynthesis by infected macrophages requires specific host phospholipases and lipid phosphatases.
a major physiological role of NAPE-PLD
Title: Isolevuglandin-modified phosphatidylethanolamine is metabolized by NAPE-hydrolyzing phospholipase D.
Phospholipase D is dispensable for superoxide production and degranulation in mouse neutrophils.
Title: Molecular mechanisms of N-formyl-methionyl-leucyl-phenylalanine-induced superoxide generation and degranulation in mouse neutrophils: phospholipase D is dispensable.

Submit: New GeneRIF Correction See all GeneRIFs (17)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Gene trapped (1)
Targeted (5) 1 citation

General gene information

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Markers

UniSTS:236317 (e-PCR)
- UniSTS:236317

AI448472 (e-PCR)
- UniSTS:179137

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables N-acylphosphatidylethanolamine-specific phospholipase D activity	IBA Inferred from Biological aspect of Ancestor more info
enables N-acylphosphatidylethanolamine-specific phospholipase D activity	IDA Inferred from Direct Assay more info	PubMed
enables N-acylphosphatidylethanolamine-specific phospholipase D activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables N-acylphosphatidylethanolamine-specific phospholipase D activity	ISO Inferred from Sequence Orthology more info
enables bile acid binding	IEA Inferred from Electronic Annotation more info
enables bile acid binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables phospholipase activity	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
involved_in N-acylethanolamine metabolic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within N-acylethanolamine metabolic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within N-acylethanolamine metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in N-acylphosphatidylethanolamine metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in N-acylphosphatidylethanolamine metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in N-acylphosphatidylethanolamine metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in N-acylphosphatidylethanolamine metabolic process	ISO Inferred from Sequence Orthology more info
involved_in N-acylphosphatidylethanolamine metabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in host-mediated regulation of intestinal microbiota composition	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of eating behavior	ISO Inferred from Sequence Orthology more info
involved_in phospholipid catabolic process	IEA Inferred from Electronic Annotation more info
involved_in phospholipid metabolic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of brown fat cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of inflammatory response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in temperature homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
located_in Golgi apparatus	ISS Inferred from Sequence or Structural Similarity more info
located_in Golgi membrane	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in early endosome	ISS Inferred from Sequence or Structural Similarity more info
located_in early endosome membrane	IEA Inferred from Electronic Annotation more info
is_active_in hippocampal mossy fiber to CA3 synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in membrane-bounded organelle	IBA Inferred from Biological aspect of Ancestor more info
located_in nuclear envelope	ISS Inferred from Sequence or Structural Similarity more info
located_in nucleoplasm	ISS Inferred from Sequence or Structural Similarity more info
is_active_in postsynaptic membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in presynapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in presynaptic membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in smooth endoplasmic reticulum membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D

Names: Metallo-beta-lactamase domain containing 1; NAPE-hydrolyzing phospholipase D

NP_001346892.1

EC 3.1.4.54

NP_001346893.1

EC 3.1.4.54

NP_001346894.1

EC 3.1.4.54

NP_001411611.1

EC 3.1.4.54

NP_848843.1

EC 3.1.4.54

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001359963.1 → NP_001346892.1 N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D isoform b

Status: VALIDATED

Description

Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2, 3, and 4 all encode the same isoform (b).

Source sequence(s)

AC125343, AC161484, AK049135

Consensus CDS

CCDS19105.1

UniProtKB/Swiss-Prot

Q8BH82

UniProtKB/TrEMBL

Q8BLU8

Related

ENSMUSP00000110872.2, ENSMUST00000115217.8

Conserved Domains (1) summary

cl23716
Location:129 → 316

metallo-hydrolase-like_MBL-fold; mainly hydrolytic enzymes and related proteins which carry out various biological functions; MBL-fold metallohydrolase domain
NM_001359964.1 → NP_001346893.1 N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D isoform a

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longer isoform (a).

Source sequence(s)

AC125343, AC161484

UniProtKB/TrEMBL

Q8BLU8

Conserved Domains (1) summary

cl23716
Location:137 → 324

metallo-hydrolase-like_MBL-fold; mainly hydrolytic enzymes and related proteins which carry out various biological functions; MBL-fold metallohydrolase domain
NM_001359965.1 → NP_001346894.1 N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D isoform b

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2, 3, and 4 all encode the same isoform (b).

Source sequence(s)

AC125343, AC161484

Consensus CDS

CCDS19105.1

UniProtKB/Swiss-Prot

Q8BH82

UniProtKB/TrEMBL

Q8BLU8

Conserved Domains (1) summary

cl23716
Location:129 → 316

metallo-hydrolase-like_MBL-fold; mainly hydrolytic enzymes and related proteins which carry out various biological functions; MBL-fold metallohydrolase domain
NM_001424682.1 → NP_001411611.1 N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D isoform b

Status: VALIDATED

Source sequence(s)

AC125343, AC161484

UniProtKB/Swiss-Prot

Q8BH82
NM_178728.6 → NP_848843.1 N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D isoform b

See identical proteins and their annotated locations for NP_848843.1

Status: VALIDATED

Description

Transcript Variant: This variant (4) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2, 3, and 4 all encode the same isoform (b).

Source sequence(s)

AC125343, AC161484, BC062890

Consensus CDS

CCDS19105.1

UniProtKB/Swiss-Prot

Q8BH82

UniProtKB/TrEMBL

Q8BLU8

Related

ENSMUSP00000054458.9, ENSMUST00000060899.9

Conserved Domains (1) summary

cl23716
Location:129 → 316

metallo-hydrolase-like_MBL-fold; mainly hydrolytic enzymes and related proteins which carry out various biological functions; MBL-fold metallohydrolase domain

RNA

NR_188845.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC125343, AC161484
NR_188846.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC125343, AC161484