Gria4 glutamate receptor, ionotropic, AMPA4 (alpha 4) [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Gria4provided by MGI
Official Full Name: glutamate receptor, ionotropic, AMPA4 (alpha 4)provided by MGI
Primary source: MGI:MGI:95811
See related: Ensembl:ENSMUSG00000025892 AllianceGenome:MGI:95811
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: GluA4; Glur4; spkw1; GluR-D; Glur-4; Gluralpha4
Summary: Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits, arranged to form ligand-gated ion channels. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The subunit encoded by this gene belongs to a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-sensitive glutamate receptors, and is subject to RNA editing (AGA->GGA; R->G). Alternative splicing of this gene results in transcript variants encoding different isoforms, which may vary in their signal transduction properties. [provided by RefSeq, Jul 2008]
Expression: Biased expression in cerebellum adult (RPKM 34.2), frontal lobe adult (RPKM 17.3) and 6 other tissues See more
Orthologs: human all
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Genomic context

Location:: 9 A1; 9 2.46 cM

Exon count:: 21

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	9	NC_000075.7 (4417893..4796250, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	9	NC_000075.6 (4417893..4796234, complement)

Chromosome 9 - NC_000075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Neuronal activity regulated pentraxin (narp) and GluA4 subunit of AMPA receptor may be targets for fluoxetine modulation.
Title: Neuronal activity regulated pentraxin (narp) and GluA4 subunit of AMPA receptor may be targets for fluoxetine modulation.
Frrsl1 deletion results in an increased proportion of immature AMPA receptors, indicated by incomplete glycosylation of GLUA2 (also known as GRIA2) and GLUA4 (also known as GRIA4) AMPA receptor proteins.
Title: Loss of Frrs1l disrupts synaptic AMPA receptor function, and results in neurodevelopmental, motor, cognitive and electrographical abnormalities.
find that during the first week in culture (DIV2-6) when GluA4 expression is restricted to pyramidal neurons, loss of GluA4 has no effect on the overall excitability of the immature network
Title: GluA4 Dependent Plasticity Mechanisms Contribute to Developmental Synchronization of the CA3-CA1 Circuitry in the Hippocampus.
Study indentifies NF-Yb as a novel transcriptional regulator of Gria4 (GluA4 gene), and a controller of excitotoxic death in the oligodendroglial lineage; describes a novel regulatory region within Gria4 containing CCAAT sequences whose binding by NF-Yb is regulated by excitotoxicity. Excitotoxicity-induced alterations in NF-Yb binding are associated with changes in Gria4 transcription.
Title: NF-Y-dependent regulation of glutamate receptor 4 expression and cell survival in cells of the oligodendrocyte lineage.
The central distribution of AMPARs is absent in GluA3-knockout mice, and gold particles are evenly distributed along the postsynaptic density. GluA4 gold labeling was homogenously distributed along both synapse types. Thus, GluA3 and GluA4 subunits are distributed at auditory nerve synapses in a target-cell-dependent manner.
Title: The number and distribution of AMPA receptor channels containing fast kinetic GluA3 and GluA4 subunits at auditory nerve synapses depend on the target cells.
These data suggest that GluA4 enables efficient homeostatic upscaling and responsiveness to temporal activity patterns during the critical period of activity-dependent refinement of the circuitry.
Title: GluA4 subunit of AMPA receptors mediates the early synaptic response to altered network activity in the developing hippocampus.
this study shows that curcumin increased the GluR4 expression in bone marrow-derived dendritic cells activated with lipopolysaccharides, which likely contributed to the mechanism of inhibiting the Th17 cell differentiation
Title: Curcumin inhibiting Th17 cell differentiation by regulating the metabotropic glutamate receptor-4 expression on dendritic cells.
GluA4 subunits are required to produce an EPSC-triggerable postsynaptic action potentials after the presynaptic action potential, while Cav3.1 expression is needed to establish the driver function of L5B-POm synapses at hyperpolarized membrane potentials
Title: Ionotropic glutamate receptor GluA4 and T-type calcium channel Cav 3.1 subunits control key aspects of synaptic transmission at the mouse L5B-POm giant synapse.
Data indicate that the AMPA receptor subunits abundance is hippocampus, GluA2 > GluA1 > GluA3 >> GluA4; cortex, GluA2 > GluA3 >/= GluA1 >> GluA4; and cerebellum, GluA2 > GluA3 >/= GluA1 > GluA4.
Title: Interaction proteomics reveals brain region-specific AMPA receptor complexes.
Here we identify a novel modifier - called "pecanex-like 2", or Pcnxl2 for short - that reduces the severity of spike-wave dischargesin the C3H substrain in which the Gria4 mutation originally arose
Title: Unraveling genetic modifiers in the gria4 mouse model of absence epilepsy.

Submit: New GeneRIF Correction See all GeneRIFs (21)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
QTL (1) 1 citation
Targeted (9) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

NoName (e-PCR)
- UniSTS:224650

Gria4 (e-PCR), detects polymorphism
- UniSTS:496046

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables AMPA glutamate receptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables AMPA glutamate receptor activity	ISO Inferred from Sequence Orthology more info
enables AMPA glutamate receptor activity	ISS Inferred from Sequence or Structural Similarity more info
enables glutamate-gated receptor activity	ISO Inferred from Sequence Orthology more info
enables glutamate-gated receptor activity	ISS Inferred from Sequence or Structural Similarity more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables ligand-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential	IBA Inferred from Biological aspect of Ancestor more info
enables transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential	IDA Inferred from Direct Assay more info	PubMed
enables transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in chemical synaptic transmission	ISO Inferred from Sequence Orthology more info
involved_in ionotropic glutamate receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in modulation of chemical synaptic transmission	IBA Inferred from Biological aspect of Ancestor more info
involved_in modulation of chemical synaptic transmission	ISO Inferred from Sequence Orthology more info
involved_in monoatomic ion transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of smooth muscle cell apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of synaptic transmission, glutamatergic	ISO Inferred from Sequence Orthology more info
involved_in regulation of postsynaptic membrane potential	IEA Inferred from Electronic Annotation more info
involved_in synaptic transmission, glutamatergic	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of AMPA glutamate receptor complex	IBA Inferred from Biological aspect of Ancestor more info
part_of AMPA glutamate receptor complex	IDA Inferred from Direct Assay more info	PubMed
part_of AMPA glutamate receptor complex	ISO Inferred from Sequence Orthology more info
located_in dendrite	ISO Inferred from Sequence Orthology more info
located_in dendrite	ISS Inferred from Sequence or Structural Similarity more info
is_active_in dendritic spine	IBA Inferred from Biological aspect of Ancestor more info
located_in dendritic spine	ISO Inferred from Sequence Orthology more info
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
part_of kainate selective glutamate receptor complex	ISO Inferred from Sequence Orthology more info
part_of kainate selective glutamate receptor complex	ISS Inferred from Sequence or Structural Similarity more info
located_in neuronal cell body	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in postsynaptic density	ISO Inferred from Sequence Orthology more info
is_active_in postsynaptic density membrane	IBA Inferred from Biological aspect of Ancestor more info
is_active_in postsynaptic density membrane	ISO Inferred from Sequence Orthology more info
located_in postsynaptic membrane	IEA Inferred from Electronic Annotation more info
is_active_in presynaptic active zone membrane	ISO Inferred from Sequence Orthology more info
located_in synapse	IDA Inferred from Direct Assay more info	PubMed
located_in synapse	ISO Inferred from Sequence Orthology more info
located_in terminal bouton	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: glutamate receptor 4

Names: AMPA-selective glutamate receptor 4; glutamate receptor channel alpha 4; glutamate receptor ionotropic, AMPA 4; spike wave 1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001113180.2 → NP_001106651.2 glutamate receptor 4 isoform 2 precursor

Status: REVIEWED

Description

Transcript Variant: This variant (2) uses an alternate internal coding exon compared to transcript variant 1, and encodes an isoform (2, also known as flop isoform) that is the same length, but with a few amino acid differences compared to isoform 1.

Source sequence(s)

AC127269, AC141562, AC156503, CT030685

Consensus CDS

CCDS52715.1

UniProtKB/TrEMBL

C9K0Y7, G5E863

Related

ENSMUSP00000027020.7, ENSMUST00000027020.13

Conserved Domains (2) summary

cd13727
Location:414 → 795

PBP2_iGluR_AMPA_GluR4; The ligand-binding domain of the AMPA (alpha-amino-3-hydroxyl-5-methyl-4-isoxazolepropionic acid) subtype GluR4 of ionotropic glutamate receptors, a member of the type 2 periplasmic binding fold protein superfamily

cd06388
Location:28 → 400

PBP1_iGluR_AMPA_GluR4; N-terminal leucine-isoleucine-valine binding protein (LIVBP)-like domain of the GluR4 subunit of the AMPA receptor
NM_001113181.1 → NP_001106652.1 glutamate receptor 4 isoform 3 precursor

Status: REVIEWED

Description

Transcript Variant: This variant (3) is missing several coding exons at the 3' end, and contains a novel 3' terminal exon compared to transcript variant 1. This results in a shorter isoform (3) with a different C-terminus compared to isoform 1.

Source sequence(s)

AK032103, BC060697, CA320207

Consensus CDS

CCDS52716.1

UniProtKB/TrEMBL

E9PX01, Q8C069

Related

ENSMUSP00000129316.2, ENSMUST00000163309.2

Conserved Domains (1) summary

cd06388
Location:28 → 400

PBP1_iGluR_AMPA_GluR4; N-terminal leucine-isoleucine-valine binding protein (LIVBP)-like domain of the GluR4 subunit of the AMPA receptor
NM_019691.5 → NP_062665.4 glutamate receptor 4 isoform 1 precursor

Status: REVIEWED

Description

Transcript Variant: This variant (1) encodes isoform 1 (also known as flip isoform).

Source sequence(s)

AC127269, AC141562, AC156503, CT030685

Consensus CDS

CCDS22797.1

UniProtKB/Swiss-Prot

Q6P9M7, Q9Z2W8

UniProtKB/TrEMBL

C9K0Y7

Related

ENSMUSP00000066980.8, ENSMUST00000063508.15

Conserved Domains (2) summary

cd13727
Location:414 → 795

PBP2_iGluR_AMPA_GluR4; The ligand-binding domain of the AMPA (alpha-amino-3-hydroxyl-5-methyl-4-isoxazolepropionic acid) subtype GluR4 of ionotropic glutamate receptors, a member of the type 2 periplasmic binding fold protein superfamily

cd06388
Location:28 → 400

PBP1_iGluR_AMPA_GluR4; N-terminal leucine-isoleucine-valine binding protein (LIVBP)-like domain of the GluR4 subunit of the AMPA receptor

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000075.7 Reference GRCm39 C57BL/6J

Range

4417893..4796250 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006509844.4 → XP_006509907.1 glutamate receptor 4 isoform X4

UniProtKB/TrEMBL

C9K0Y7

Conserved Domains (2) summary

cd13727
Location:414 → 795

PBP2_iGluR_AMPA_GluR4; The ligand-binding domain of the AMPA (alpha-amino-3-hydroxyl-5-methyl-4-isoxazolepropionic acid) subtype GluR4 of ionotropic glutamate receptors, a member of the type 2 periplasmic binding fold protein superfamily

cd06388
Location:28 → 400

PBP1_iGluR_AMPA_GluR4; N-terminal leucine-isoleucine-valine binding protein (LIVBP)-like domain of the GluR4 subunit of the AMPA receptor
XM_006509843.4 → XP_006509906.1 glutamate receptor 4 isoform X3

UniProtKB/TrEMBL

Q8C0E4

Conserved Domains (2) summary

cd13727
Location:414 → 795

PBP2_iGluR_AMPA_GluR4; The ligand-binding domain of the AMPA (alpha-amino-3-hydroxyl-5-methyl-4-isoxazolepropionic acid) subtype GluR4 of ionotropic glutamate receptors, a member of the type 2 periplasmic binding fold protein superfamily

cd06388
Location:28 → 400

PBP1_iGluR_AMPA_GluR4; N-terminal leucine-isoleucine-valine binding protein (LIVBP)-like domain of the GluR4 subunit of the AMPA receptor
XM_006509841.4 → XP_006509904.1 glutamate receptor 4 isoform X1

UniProtKB/TrEMBL

Q8C0E4

Conserved Domains (2) summary

cd13727
Location:414 → 795

PBP2_iGluR_AMPA_GluR4; The ligand-binding domain of the AMPA (alpha-amino-3-hydroxyl-5-methyl-4-isoxazolepropionic acid) subtype GluR4 of ionotropic glutamate receptors, a member of the type 2 periplasmic binding fold protein superfamily

cd06388
Location:28 → 400

PBP1_iGluR_AMPA_GluR4; N-terminal leucine-isoleucine-valine binding protein (LIVBP)-like domain of the GluR4 subunit of the AMPA receptor
XM_006509842.4 → XP_006509905.1 glutamate receptor 4 isoform X2

UniProtKB/TrEMBL

C9K0Y7

Conserved Domains (2) summary

cd13727
Location:414 → 795

PBP2_iGluR_AMPA_GluR4; The ligand-binding domain of the AMPA (alpha-amino-3-hydroxyl-5-methyl-4-isoxazolepropionic acid) subtype GluR4 of ionotropic glutamate receptors, a member of the type 2 periplasmic binding fold protein superfamily

cd06388
Location:28 → 400

PBP1_iGluR_AMPA_GluR4; N-terminal leucine-isoleucine-valine binding protein (LIVBP)-like domain of the GluR4 subunit of the AMPA receptor
XM_011242355.4 → XP_011240657.1 glutamate receptor 4 isoform X6

See identical proteins and their annotated locations for XP_011240657.1

UniProtKB/TrEMBL

Q8C0E4

Conserved Domains (2) summary

cd06388
Location:28 → 400

PBP1_iGluR_AMPA_GluR4; N-terminal leucine-isoleucine-valine binding protein (LIVBP)-like domain of the GluR4 subunit of the AMPA receptor

cl21456
Location:414 → 765

Periplasmic_Binding_Protein_Type_2; Type 2 periplasmic binding fold superfamily
XM_006509845.3 → XP_006509908.1 glutamate receptor 4 isoform X5

UniProtKB/TrEMBL

C9K0Y7

Conserved Domains (2) summary

cd13727
Location:414 → 795

PBP2_iGluR_AMPA_GluR4; The ligand-binding domain of the AMPA (alpha-amino-3-hydroxyl-5-methyl-4-isoxazolepropionic acid) subtype GluR4 of ionotropic glutamate receptors, a member of the type 2 periplasmic binding fold protein superfamily

cd06388
Location:28 → 400

PBP1_iGluR_AMPA_GluR4; N-terminal leucine-isoleucine-valine binding protein (LIVBP)-like domain of the GluR4 subunit of the AMPA receptor