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    Ephx1 epoxide hydrolase 1, microsomal [ Mus musculus (house mouse) ]

    Gene ID: 13849, updated on 2-Nov-2024

    Summary

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    Official Symbol
    Ephx1provided by MGI
    Official Full Name
    epoxide hydrolase 1, microsomalprovided by MGI
    Primary source
    MGI:MGI:95405
    See related
    Ensembl:ENSMUSG00000038776 AllianceGenome:MGI:95405
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    EH; mEH; Eph1; Eph-1; Epxh1
    Summary
    Enables oxysterol binding activity. Acts upstream of or within hydrocarbon catabolic process and response to polycyclic arene. Predicted to be located in intracellular membrane-bounded organelle and membrane. Is expressed in several structures, including brain ventricle and choroid plexus; exocrine system; integumental system; nervous system; and nose. Human ortholog(s) of this gene implicated in several diseases, including Leber hereditary optic neuropathy; anemia (multiple); hematologic cancer (multiple); respiratory system disease (multiple); and toxic encephalopathy. Orthologous to human EPHX1 (epoxide hydrolase 1). [provided by Alliance of Genome Resources, Nov 2024]
    Expression
    Broad expression in liver adult (RPKM 113.7), bladder adult (RPKM 62.6) and 19 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Ephx1 in Genome Data Viewer
    Location:
    1 H4; 1 84.48 cM
    Exon count:
    9
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 1 NC_000067.7 (180817121..180845134, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 1 NC_000067.6 (180989556..181017569, complement)

    Chromosome 1 - NC_000067.7Genomic Context describing neighboring genes Neighboring gene predicted gene, 39715 Neighboring gene left right determination factor 1 Neighboring gene transmembrane protein 63a Neighboring gene STARR-seq mESC enhancer starr_03223 Neighboring gene STARR-seq mESC enhancer starr_03226 Neighboring gene STARR-positive B cell enhancer ABC_E3344 Neighboring gene STARR-seq mESC enhancer starr_03227 Neighboring gene STARR-seq mESC enhancer starr_03228 Neighboring gene delta 4-desaturase, sphingolipid 1 like Neighboring gene predicted gene, 39716 Neighboring gene ribosomal protein L7 pseudogene

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Male mice deficient in microsomal epoxide hydrolase are not susceptible to benzene-induced toxicity. Bauer AK, et al. Toxicol Sci, 2003 Apr. PMID 12655032
    2. Hepatitis B spliced protein (HBSP) promotes the carcinogenic effects of benzo [alpha] pyrene by interacting with microsomal epoxide hydrolase and enhancing its hydrolysis activity. Chen JY, et al. BMC Cancer, 2014 Apr 23. PMID 24758376, Free PMC Article
    3. Cloning of epoxide hydratase complementary DNA. Gonzalez FJ, et al. J Biol Chem, 1981 May 25. PMID 6262298
    4. Microsomal expoxide hydrolase is required for 7,12-dimethylbenz[a]anthracene (DMBA)-induced immunotoxicity in mice. Gao J, et al. Toxicol Sci, 2007 Jul. PMID 17442664
    5. Lipidomic profiling reveals soluble epoxide hydrolase as a therapeutic target of obesity-induced colonic inflammation. Wang W, et al. Proc Natl Acad Sci U S A, 2018 May 15. PMID 29717038, Free PMC Article

    See all (55) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Soluble Epoxide Hydrolase in Aged Female Mice and Human Explanted Hearts Following Ischemic Injury.
      Title: Soluble Epoxide Hydrolase in Aged Female Mice and Human Explanted Hearts Following Ischemic Injury.
    2. Microsomal epoxide hydrolase forms complex with CYP2J5 in the endoplasmic reticulum.
      Title: Evidence for a complex formation between CYP2J5 and mEH in living cells by FRET analysis of membrane protein interaction in the endoplasmic reticulum (FAMPIR).
    3. High epoxide hydrolase expression is associated with colonic inflammation.
      Title: Lipidomic profiling reveals soluble epoxide hydrolase as a therapeutic target of obesity-induced colonic inflammation.
    4. MEH appears to play a leading role in the hydrolysis of 8,9-epoxyeicosatrienoic acid and also contributes to the hydrolysis of other fatty acid epoxides.
      Title: Beyond detoxification: a role for mouse mEH in the hepatic metabolism of endogenous lipids.
    5. These findings show the critical role of soluble epoxide hydrolase S-nitrosation in cardiac ischemia-reperfusion injury.
      Title: Soluble epoxide hydrolase activation by S-nitrosation contributes to cardiac ischemia-reperfusion injury.
    6. Data, including data from mutant strain of mice, suggest critical role of Ephx1 in regulation of vasodynamics (specifically regulation of cerebral blood flow) and suggest that deregulation of endogenous signaling pathways (such as eicosanoid metabolism) is undesirable gain of function associated with E404D variant of Ephx1.
      Title: Genetic enhancement of microsomal epoxide hydrolase improves metabolic detoxification but impairs cerebral blood flow regulation.
    7. Six proteins were regulated at both basal and inducible levels exhibiting the largest dynamic range of Nrf2 regulation: cytochrome CYP2A5, GSTM3, GSTM1, ENTPD5,UDPGDH, and EPHX1.
      Title: Identification and quantification of the basal and inducible Nrf2-dependent proteomes in mouse liver: biochemical, pharmacological and toxicological implications.
    8. Expression of HBSP significantly increased the formation of BPDE-DNA adduct in benzo[alpha]pyrene-treated Huh-7 hepatoma cells, and this enhancement was blocked by knockdown of microsomal epoxide hydrolase
      Title: Hepatitis B spliced protein (HBSP) promotes the carcinogenic effects of benzo [alpha] pyrene by interacting with microsomal epoxide hydrolase and enhancing its hydrolysis activity.
    9. In livers of HRN mice Cyp1a1, cytochrome b5 and mEH can effectively activate BaP to DNA binding species, even in the presence of very low amounts of P450 oxidoreductase.
      Title: Cytochrome b5 and epoxide hydrolase contribute to benzo[a]pyrene-DNA adduct formation catalyzed by cytochrome P450 1A1 under low NADPH:P450 oxidoreductase conditions.
    10. The results suggest that epoxide intermediates mediate methamphetamine drug dependence and that astrocytic reactions of microsomal epoxide hydrolase protein are important in the endogenous modulation in response to methamphetamine drug dependence.
      Title: Role of microsomal epoxide hydrolase in methamphetamine-induced drug dependence in mice.
    Submit: New GeneRIF Correction See all GeneRIFs (13)

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (5) 
    • Targeted (3)  1 citation

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables cis-stilbene-oxide hydrolase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables cis-stilbene-oxide hydrolase activity ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    enables enzyme binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables epoxide hydrolase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables epoxide hydrolase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables epoxide hydrolase activity ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    enables oxysterol binding IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in arachidonate metabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in arachidonate metabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in arachidonate metabolic process ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    involved_in diol biosynthetic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in epoxide metabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in epoxide metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in epoxide metabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within hydrocarbon catabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within response to polycyclic arene IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in response to toxic substance IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in endoplasmic reticulum membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in intracellular membrane-bounded organelle ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in membrane ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    epoxide hydrolase 1
    Names
    epoxide hydratase
    microsomal epoxide hydrolase
    NP_001299847.1
    NP_034275.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001312918.1 → NP_001299847.1  epoxide hydrolase 1 isoform 2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) uses an alternate in-frame splice site compared to variant 1. The encoded isoform (2) is shorter than isoform 1.
      Source sequence(s)
      AC117673, AK018249, AW047588, BY312366
      Consensus CDS
      CCDS83653.1
      UniProtKB/TrEMBL
      E9PWK1, Q6PEV0
      Related
      ENSMUSP00000106697.2, ENSMUST00000111068.8
      Conserved Domains (3) summary
      COG0596
      Location:121 → 434
      MhpC; Pimeloyl-ACP methyl ester carboxylesterase [Coenzyme transport and metabolism, General function prediction only]
      pfam06441
      Location:50 → 152
      EHN; Epoxide hydrolase N terminus
      cl21494
      Location:142 → 231
      Abhydrolase; alpha/beta hydrolases

    2. NM_010145.3 → NP_034275.1  epoxide hydrolase 1 isoform 1

      See identical proteins and their annotated locations for NP_034275.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
      Source sequence(s)
      AC117673, AK018249, AW047588, BY312366
      Consensus CDS
      CCDS15579.1
      UniProtKB/Swiss-Prot
      P97869, Q9D379
      UniProtKB/TrEMBL
      Q8K2W5
      Related
      ENSMUSP00000047551.6, ENSMUST00000036928.12
      Conserved Domains (3) summary
      COG0596
      Location:121 → 448
      MhpC; Pimeloyl-ACP methyl ester carboxylesterase [Coenzyme transport and metabolism, General function prediction only]
      pfam06441
      Location:50 → 152
      EHN; Epoxide hydrolase N terminus
      cl21494
      Location:124 → 255
      Abhydrolase; alpha/beta hydrolases

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000067.7 Reference GRCm39 C57BL/6J

      Range
      180817121..180845134 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9D379.2 GenPept UniProtKB/Swiss-Prot:Q9D379

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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