MISP mitotic spindle positioning [Homo sapiens (human)] - Gene

Summary

Official Symbol: MISPprovided by HGNC
Official Full Name: mitotic spindle positioningprovided by HGNC
Primary source: HGNC:HGNC:27000
See related: Ensembl:ENSG00000099812 MIM:615289; AllianceGenome:HGNC:27000
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: MISP1; C19orf21
Summary: The protein encoded by this gene is an actin-bundling protein involved in determining cell morphology and mitotic progression. The encoded protein is required for the proper positioning of the mitotic spindle. Two transcript variants, one protein-coding and the other non-protein coding, have been found for this gene. [provided by RefSeq, Feb 2016]
Expression: Biased expression in small intestine (RPKM 92.0), duodenum (RPKM 77.1) and 6 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 19p13.3

Exon count:: 7

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	19	NC_000019.10 (748438..764318)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	19	NC_060943.1 (702466..718374)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	19	NC_000019.9 (751112..764318)

Chromosome 19 - NC_000019.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

MISP Is Overexpressed in Intestinal Metaplasia and Gastric Cancer.
Title: MISP Is Overexpressed in Intestinal Metaplasia and Gastric Cancer.
Mitotic spindle positioning protein (MISP) preferentially binds to aged F-actin.
Title: Mitotic spindle positioning protein (MISP) preferentially binds to aged F-actin.
PLK1 and its substrate MISP facilitate intrahepatic cholangiocarcinoma progression by promoting lymphatic invasion and impairing E-cadherin adherens junctions.
Title: PLK1 and its substrate MISP facilitate intrahepatic cholangiocarcinoma progression by promoting lymphatic invasion and impairing E-cadherin adherens junctions.
Plants distinguish different photoperiods to independently control seasonal flowering and growth.
Title: Plants distinguish different photoperiods to independently control seasonal flowering and growth.
Modulation of actin-binding and -bundling activities of MISP/Caprice by multiple phosphorylation.
Title: Modulation of actin-binding and -bundling activities of MISP/Caprice by multiple phosphorylation.
MISP directly interacts with ezrin and that SLK/LOK-activated ezrin ensures appropriate cortical MISP levels in mitosis by competing with MISP for actin-binding sites at the cell cortex.
Title: Activated ezrin controls MISP levels to ensure correct NuMA polarization and spindle orientation.
MISP is an effector for cell type-specific actin reorganization with its direct actin-binding properties and provides a novel model of cell morphology regulation by a non-ubiquitous single actin-bundling protein.
Title: Caprice/MISP is a novel F-actin bundling protein critical for actin-based cytoskeletal reorganizations.
Bundles F-actin and balances stress fiber-filopodial formation
Title: Caprice/MISP is a novel F-actin bundling protein critical for actin-based cytoskeletal reorganizations.
MISP links microtubules to the actin cytoskeleton and focal adhesions in order to properly position the mitotic spindle.
Title: The novel actin/focal adhesion-associated protein MISP is involved in mitotic spindle positioning in human cells.
Plk1 phosphorylates MISP, thus stabilizing cortical and astral microtubule attachments required for proper mitotic spindle positioning.
Title: MISP is a novel Plk1 substrate required for proper spindle orientation and mitotic progression.

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Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Protein interactions

Protein	Gene	Interaction	Pubs
Vpr	vpr	HIV-1 Vpr upregulates MISP (C19orf21) in HeLa cells within 12 hours of exposure	PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables actin filament binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of centrosome localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of mitotic spindle orientation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic spindle assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in organelle localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of protein localization to cell cortex	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in actin filament	IMP Inferred from Mutant Phenotype more info	PubMed
colocalizes_with cortical actin cytoskeleton	IMP Inferred from Mutant Phenotype more info	PubMed
located_in focal adhesion	IDA Inferred from Direct Assay more info
colocalizes_with focal adhesion	IMP Inferred from Mutant Phenotype more info	PubMed
located_in focal adhesion	IMP Inferred from Mutant Phenotype more info	PubMed
located_in intracellular membrane-bounded organelle	IDA Inferred from Direct Assay more info
colocalizes_with mitotic spindle astral microtubule end	IMP Inferred from Mutant Phenotype more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info
located_in spindle pole centrosome	IMP Inferred from Mutant Phenotype more info	PubMed

General protein information

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Preferred Names: mitotic interactor and substrate of PLK1

Names: caprice; mitotic interactor and substrate of Plk1; mitotic spindle positioning protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_173481.4 → NP_775752.1 mitotic interactor and substrate of PLK1

See identical proteins and their annotated locations for NP_775752.1

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longer, protein-coding transcript.

Source sequence(s)

AC004030, BC052236, HY163020

Consensus CDS

CCDS12042.1

UniProtKB/Swiss-Prot

Q8IVT2

Related

ENSP00000215582.4, ENST00000215582.8

Conserved Domains (1) summary

pfam15304
Location:325 → 674

AKAP2_C; A-kinase anchor protein 2 C-terminus

RNA

NR_135168.2 RNA Sequence

Status: REVIEWED

Description

Transcript Variant: This variant (2) lacks an alternate coding exon compared to variant 1. This variant is represented as non-coding because it lacks a large portion of the coding region, including the translational start codon, found in variant 1.

Source sequence(s)

AC004030, BC052236, HY163020, HY173833

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

NC_000019.10 Reference GRCh38.p14 Primary Assembly

Range

748438..764318

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011527685.3 → XP_011525987.1 mitotic interactor and substrate of PLK1 isoform X1

See identical proteins and their annotated locations for XP_011525987.1

UniProtKB/Swiss-Prot

Q8IVT2

Conserved Domains (1) summary

pfam15304
Location:325 → 674

AKAP2_C; A-kinase anchor protein 2 C-terminus
XM_011527686.3 → XP_011525988.1 mitotic interactor and substrate of PLK1 isoform X1

See identical proteins and their annotated locations for XP_011525988.1

UniProtKB/Swiss-Prot

Q8IVT2

Conserved Domains (1) summary

pfam15304
Location:325 → 674

AKAP2_C; A-kinase anchor protein 2 C-terminus

Alternate T2T-CHM13v2.0

Genomic

NC_060943.1 Alternate T2T-CHM13v2.0

Range

702466..718374

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_054319805.1 → XP_054175780.1 mitotic interactor and substrate of PLK1 isoform X1

UniProtKB/Swiss-Prot

Q8IVT2
XM_054319806.1 → XP_054175781.1 mitotic interactor and substrate of PLK1 isoform X1

UniProtKB/Swiss-Prot

Q8IVT2