Chrna5 cholinergic receptor, nicotinic, alpha polypeptide 5 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Chrna5provided by MGI
Official Full Name: cholinergic receptor, nicotinic, alpha polypeptide 5provided by MGI
Primary source: MGI:MGI:87889
See related: Ensembl:ENSMUSG00000035594 AllianceGenome:MGI:87889
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Acra5; Acra-5
Summary: Predicted to enable acetylcholine binding activity and acetylcholine-gated monoatomic cation-selective channel activity. Predicted to contribute to acetylcholine receptor activity. Involved in presynaptic modulation of chemical synaptic transmission and regulation of postsynaptic neurotransmitter receptor activity. Acts upstream of or within response to nicotine. Part of acetylcholine-gated channel complex. Is active in cholinergic synapse and dopaminergic synapse. Is expressed in several structures, including brain; gut; lung; trigeminal nerve; and vagus ganglion. Human ortholog(s) of this gene implicated in chronic obstructive pulmonary disease; cocaine abuse; esophagus squamous cell carcinoma; nicotine dependence; and respiratory system cancer (multiple). Orthologous to human CHRNA5 (cholinergic receptor nicotinic alpha 5 subunit). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in testis adult (RPKM 2.0), CNS E18 (RPKM 0.5) and 6 other tissues See more
Orthologs: human all
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Genomic context

Location:: 9 B; 9 29.84 cM

Exon count:: 7

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	9	NC_000075.7 (54888112..54918704)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	9	NC_000075.6 (54980827..55011420)

Chromosome 9 - NC_000075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

CHRNA5 gene variation affects the response of VTA dopaminergic neurons during chronic nicotine exposure and withdrawal.
Title: CHRNA5 gene variation affects the response of VTA dopaminergic neurons during chronic nicotine exposure and withdrawal.
CHRNA5 Is Overexpressed in Patients with Psoriasis and Promotes Psoriasis-Like Inflammation in Mouse Models.
Title: CHRNA5 Is Overexpressed in Patients with Psoriasis and Promotes Psoriasis-Like Inflammation in Mouse Models.
A common SNP in Chrna5 enhances morphine reward in female mice.
Title: A common SNP in Chrna5 enhances morphine reward in female mice.
Mutation of the alpha5 nicotinic acetylcholine receptor subunit increases ethanol and nicotine consumption in adolescence and impacts adult drug consumption.
Title: Mutation of the α5 nicotinic acetylcholine receptor subunit increases ethanol and nicotine consumption in adolescence and impacts adult drug consumption.
Chrna5 is Essential for a Rapid and Protected Response to Optogenetic Release of Endogenous Acetylcholine in Prefrontal Cortex.
Title: Chrna5 is Essential for a Rapid and Protected Response to Optogenetic Release of Endogenous Acetylcholine in Prefrontal Cortex.
The majority of nicotinic receptors at the cell surface of superior cervical ganglia neurons consist of alpha3beta4alpha5 subunits.
Title: The role of the nAChR subunits α5, β2, and β4 on synaptic transmission in the mouse superior cervical ganglion.
Deletion of the alpha5 subunit in mice markedly reduces the cellular response to nicotine and acetylcholine in the interpeduncular nucleus. Stimulation of alpha5-expressing IP neurons using optogenetics is aversive, but this effect requires priming by recent prior stimulation or exposure to nicotine. The smoking-associated variant of the alpha5 gene may increase the drive to smoke via loss of IP-mediated nicotine avers...
Title: Chrna5-Expressing Neurons in the Interpeduncular Nucleus Mediate Aversion Primed by Prior Stimulation or Nicotine Exposure.
Observations in hemiparkinsonian mice suggest that the lack of alpha5* nicotinic receptors results in attenuated dopaminergic neurodegeneration and motor dysfunction in a 6-OHDA neurotoxin model of Parkinson's disease; suggests potential for alpha5 subunit-containing nicotinic receptors as a novel target in the treatment of Parkinson's disease.
Title: Attenuated dopaminergic neurodegeneration and motor dysfunction in hemiparkinsonian mice lacking the α5 nicotinic acetylcholine receptor subunit.
Data suggest that the Chrna5 D398N variant influences the effect of developmental nicotine exposure on nicotine intake of exposed offspring. This gene-environment interaction on striatal DA release may provide motivation for increased nicotine seeking in N Nic offspring and reduced consumption in D Nic offspring.
Title: The interaction of the Chrna5 D398N variant with developmental nicotine exposure.
The findings reveal a proaddiction adaptive response to chronic nicotine in which nitric oxide and SST are released by a specific CHRNA5-expressing neuronal population to provide retrograde inhibition of the habenula- interpeduncular nucleus circuit and thereby enhance the motivational properties of nicotine.
Title: Retrograde inhibition by a specific subset of interpeduncular α5 nicotinic neurons regulates nicotine preference.

Submit: New GeneRIF Correction See all GeneRIFs (30)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3) 1 citation
Targeted (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

NoName (e-PCR)
- UniSTS:489548

Chrna5 (e-PCR), detects polymorphism
- UniSTS:545913

Chrna5 (e-PCR), detects polymorphism
- UniSTS:532478

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables acetylcholine binding	ISO Inferred from Sequence Orthology more info
contributes_to acetylcholine receptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables acetylcholine receptor activity	IEA Inferred from Electronic Annotation more info
enables acetylcholine receptor activity	ISO Inferred from Sequence Orthology more info
enables acetylcholine-gated monoatomic cation-selective channel activity	IBA Inferred from Biological aspect of Ancestor more info
enables acetylcholine-gated monoatomic cation-selective channel activity	IEA Inferred from Electronic Annotation more info
enables acetylcholine-gated monoatomic cation-selective channel activity	ISO Inferred from Sequence Orthology more info
enables protein-containing complex binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in acetylcholine receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in behavioral response to nicotine	IEA Inferred from Electronic Annotation more info
involved_in behavioral response to nicotine	ISO Inferred from Sequence Orthology more info
involved_in cellular response to nicotine	ISO Inferred from Sequence Orthology more info
involved_in detection of mechanical stimulus involved in sensory perception of pain	ISO Inferred from Sequence Orthology more info
involved_in excitatory postsynaptic potential	IEA Inferred from Electronic Annotation more info
involved_in membrane depolarization	IBA Inferred from Biological aspect of Ancestor more info
involved_in monoatomic ion transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in monoatomic ion transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in presynaptic modulation of chemical synaptic transmission	IDA Inferred from Direct Assay more info	PubMed
involved_in presynaptic modulation of chemical synaptic transmission	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of neuronal action potential	ISO Inferred from Sequence Orthology more info
involved_in regulation of postsynaptic neurotransmitter receptor activity	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of postsynaptic neurotransmitter receptor activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to nicotine	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within response to nicotine	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in synaptic transmission, cholinergic	IBA Inferred from Biological aspect of Ancestor more info
involved_in synaptic transmission, cholinergic	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of acetylcholine-gated channel complex	IBA Inferred from Biological aspect of Ancestor more info
part_of acetylcholine-gated channel complex	IPI Inferred from Physical Interaction more info	PubMed
part_of acetylcholine-gated channel complex	ISO Inferred from Sequence Orthology more info
is_active_in cholinergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in cholinergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
located_in dendrite	ISO Inferred from Sequence Orthology more info
is_active_in dopaminergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in dopaminergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in neuron projection	IBA Inferred from Biological aspect of Ancestor more info
located_in neuronal cell body	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in postsynaptic membrane	IEA Inferred from Electronic Annotation more info
located_in presynapse	IEA Inferred from Electronic Annotation more info
is_active_in synapse	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: neuronal acetylcholine receptor subunit alpha-5

Names: nicotinic acetylcholine receptor alpha5 subunit

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001373901.1 → NP_001360830.1 neuronal acetylcholine receptor subunit alpha-5 isoform 1 precursor

Status: VALIDATED

Source sequence(s)

AC161264

UniProtKB/Swiss-Prot

Q2MKA5, Q6PW45

UniProtKB/TrEMBL

Q9QXK6

Related

ENSMUSP00000150942.2, ENSMUST00000213960.2

Conserved Domains (2) summary

cl28912
Location:45 → 251

LGIC_ECD; extracellular domain (ECD) of Cys-loop neurotransmitter-gated ion channels (also known as ligand-gated ion channel (LGIC))

pfam02932
Location:257 → 445

Neur_chan_memb; Neurotransmitter-gated ion-channel transmembrane region
NM_001373902.1 → NP_001360831.1 neuronal acetylcholine receptor subunit alpha-5 isoform 3 precursor

Status: VALIDATED

Source sequence(s)

AC161264

UniProtKB/TrEMBL

A0A1L1SR97, Q8BIE9

Related

ENSMUSP00000149366.2, ENSMUST00000217408.2

Conserved Domains (1) summary

TIGR00860
Location:36 → 344

LIC; Cation transporter family protein
NM_176844.5 → NP_789814.2 neuronal acetylcholine receptor subunit alpha-5 isoform 2

See identical proteins and their annotated locations for NP_789814.2

Status: VALIDATED

Source sequence(s)

AC161264

Consensus CDS

CCDS52800.1

UniProtKB/TrEMBL

Q3KP94

Related

ENSMUSP00000091365.4, ENSMUST00000093844.5

Conserved Domains (2) summary

cl28912
Location:16 → 222

LGIC_ECD; extracellular domain (ECD) of Cys-loop neurotransmitter-gated ion channels (also known as ligand-gated ion channel (LGIC))

pfam02932
Location:228 → 416

Neur_chan_memb; Neurotransmitter-gated ion-channel transmembrane region

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000075.7 Reference GRCm39 C57BL/6J

Range

54888112..54918704

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030244017.2 → XP_030099877.1 neuronal acetylcholine receptor subunit alpha-5 isoform X1

UniProtKB/TrEMBL

Q9QXK6

Conserved Domains (3) summary

TIGR00860
Location:1 → 356

LIC; Cation transporter family protein

cd03558
Location:78 → 92

LGIC_ECD; Cys-loop [structural motif]

cl28912
Location:1 → 159

LGIC_ECD; extracellular domain (ECD) of Cys-loop neurotransmitter-gated ion channels (also known as ligand-gated ion channel (LGIC))