Clcnkb chloride channel, voltage-sensitive Kb [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Clcnkbprovided by MGI
Official Full Name: chloride channel, voltage-sensitive Kbprovided by MGI
Primary source: MGI:MGI:1930643
See related: Ensembl:ENSMUSG00000006216 AllianceGenome:MGI:1930643
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: CLCKB; Clck2; Clc-Ka; Clcnk2; Clcnk1l
Summary: This gene is a member of the CLC family of voltage-gated chloride channels. The gene is located adjacent to a highly similar chloride channel gene on chromosome 4. This gene is syntenic with human CLCNKA (geneID:1187). [provided by RefSeq, Sep 2009]
Expression: Restricted expression toward kidney adult (RPKM 97.2) See more
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Genomic context

Location:: 4 D3; 4 74.02 cM

Exon count:: 21

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	4	NC_000070.7 (141131668..141143440, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	4	NC_000070.6 (141404357..141416055, complement)

Chromosome 4 - NC_000070.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Angiotensin II increases activity of the ClC-K2 Cl(-) channel in collecting duct intercalated cells by stimulating production of reactive oxygen species.
Title: Angiotensin II increases activity of the ClC-K2 Cl(-) channel in collecting duct intercalated cells by stimulating production of reactive oxygen species.
Loss of ClC-K2 compromises TAL function and abolishes salt reabsorption in the distal convoluted tubule. Our data suggest that ClC-K2 is crucial for renal salt reabsorption and concentrating ability. ClC-K2-deficient mice in most aspects mimic patients with Bartter's syndrome type 3.
Title: Salt-losing nephropathy in mice with a null mutation of the Clcnk2 gene.
Through the combined effects of pHi and pHo on ClC-K2, might be a key regulator of NaCl absorption.
Title: Dual regulation of the native ClC-K2 chloride channel in the distal nephron by voltage and pH.
IGF-1, by stimulating ClC-K2 channels, promotes net Na(+) and Cl(-) reabsorption, thus reducing driving force for potassium secretion by the cortical collecting duct.
Title: IGF-1 and insulin exert opposite actions on ClC-K2 activity in the cortical collecting ducts.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (4) 1 citation
Targeted (2) 1 citation

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables chloride channel activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables chloride channel activity	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables voltage-gated chloride channel activity	IBA Inferred from Biological aspect of Ancestor more info
enables voltage-gated chloride channel activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in chloride transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in chloride transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in chloride transport	ISO Inferred from Sequence Orthology more info
involved_in regulation of intracellular pH	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in renal absorption	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transepithelial chloride transport	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in basolateral plasma membrane	IEA Inferred from Electronic Annotation more info
part_of chloride channel complex	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: chloride channel protein ClC-Kb

Names: chloride channel K1-like; putative basolateral cTAL chloride channel ClC-Ka; chloride channel Kb; clC-K2; putative basolateral mTAL chloride channel ClC-Ka

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_019701.2 → NP_062675.2 chloride channel protein ClC-Kb

See identical proteins and their annotated locations for NP_062675.2

Status: VALIDATED

Source sequence(s)

AA240610, AK146876, BB845352, BX636867

Consensus CDS

CCDS18872.1

UniProtKB/Swiss-Prot

A2ADB6, Q8VCF8, Q9WUB6

Related

ENSMUSP00000006378.3, ENSMUST00000006378.9

Conserved Domains (2) summary

cd03683
Location:49 → 533

ClC_1_like; ClC-1-like chloride channel proteins. This CD includes isoforms ClC-0, ClC-1, ClC-2 and ClC_K. ClC-1 is expressed in skeletal muscle and its mutation leads to both recessively and dominantly-inherited forms of muscle stiffness or myotonia. ClC-K is ...

cd04591
Location:546 → 676

CBS_pair_voltage-gated_CLC_euk_bac; Two tandem repeats of the cystathionine beta-synthase (CBS pair) domains associated with the voltage gated CLC (chloride channel) in eukaryotes and bacteria

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000070.7 Reference GRCm39 C57BL/6J

Range

141131668..141143440 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006539067.4 → XP_006539130.1 chloride channel protein ClC-Kb isoform X3

Conserved Domains (2) summary

cd03683
Location:49 → 475

ClC_1_like; ClC-1-like chloride channel proteins. This CD includes isoforms ClC-0, ClC-1, ClC-2 and ClC_K. ClC-1 is expressed in skeletal muscle and its mutation leads to both recessively and dominantly-inherited forms of muscle stiffness or myotonia. ClC-K is ...

cd04591
Location:488 → 618

CBS_pair_voltage-gated_CLC_euk_bac; Two tandem repeats of the cystathionine beta-synthase (CBS pair) domains associated with the voltage gated CLC (chloride channel) in eukaryotes and bacteria
XM_006539066.4 → XP_006539129.1 chloride channel protein ClC-Kb isoform X2

See identical proteins and their annotated locations for XP_006539129.1

Conserved Domains (2) summary

cd03683
Location:49 → 533

ClC_1_like; ClC-1-like chloride channel proteins. This CD includes isoforms ClC-0, ClC-1, ClC-2 and ClC_K. ClC-1 is expressed in skeletal muscle and its mutation leads to both recessively and dominantly-inherited forms of muscle stiffness or myotonia. ClC-K is ...

cd04591
Location:546 → 648

CBS_pair_voltage-gated_CLC_euk_bac; Two tandem repeats of the cystathionine beta-synthase (CBS pair) domains associated with the voltage gated CLC (chloride channel) in eukaryotes and bacteria
XM_011250307.3 → XP_011248609.1 chloride channel protein ClC-Kb isoform X1

See identical proteins and their annotated locations for XP_011248609.1

UniProtKB/Swiss-Prot

A2ADB6, Q8VCF8, Q9WUB6

Conserved Domains (2) summary

cd03683
Location:49 → 533

ClC_1_like; ClC-1-like chloride channel proteins. This CD includes isoforms ClC-0, ClC-1, ClC-2 and ClC_K. ClC-1 is expressed in skeletal muscle and its mutation leads to both recessively and dominantly-inherited forms of muscle stiffness or myotonia. ClC-K is ...

cd04591
Location:546 → 676

CBS_pair_voltage-gated_CLC_euk_bac; Two tandem repeats of the cystathionine beta-synthase (CBS pair) domains associated with the voltage gated CLC (chloride channel) in eukaryotes and bacteria
XM_006539068.4 → XP_006539131.1 chloride channel protein ClC-Kb isoform X4

Conserved Domains (1) summary

cl02915
Location:49 → 341

Voltage_gated_ClC; CLC voltage-gated chloride channel. The ClC chloride channels catalyse the selective flow of Cl- ions across cell membranes, thereby regulating electrical excitation in skeletal muscle and the flow of salt and water across epithelial barriers. This ...