Spp1 secreted phosphoprotein 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Spp1provided by MGI
Official Full Name: secreted phosphoprotein 1provided by MGI
Primary source: MGI:MGI:98389
See related: Ensembl:ENSMUSG00000029304 AllianceGenome:MGI:98389
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: OP; 2AR; Bsp; Eta; Opn; Ric; BNSP; BSPI; Opnl; Apl-1; ETA-1; Spp-1
Summary: Enables extracellular matrix binding activity and integrin binding activity. Involved in bone mineralization and calcium ion homeostasis. Acts upstream of or within several processes, including cellular response to leukemia inhibitory factor; intracellular chloride ion homeostasis; and intracellular monoatomic cation homeostasis. Located in apical part of cell and cytoplasm. Is expressed in several structures, including alimentary system; brain; metanephros; sensory organ; and skeleton. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); biliary atresia; coronary artery disease (multiple); disease of cellular proliferation (multiple); and hepatitis. Orthologous to human SPP1 (secreted phosphoprotein 1). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in kidney adult (RPKM 580.4), liver E18 (RPKM 36.2) and 1 other tissue See more
Orthologs: human all
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Genomic context

Location:: 5 E5; 5 50.68 cM

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (104582977..104588919)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (104435111..104441053)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Intracellular osteopontin potentiates the immunosuppressive activity of mesenchymal stromal cells.
Title: Intracellular osteopontin potentiates the immunosuppressive activity of mesenchymal stromal cells.
OPN, BSP, and Bone Quality-Structural, Biochemical, and Biomechanical Assessment in OPN[-/-], BSP[-/-], and DKO Mice.
Title: OPN, BSP, and Bone Quality-Structural, Biochemical, and Biomechanical Assessment in OPN(-/-), BSP(-/-), and DKO Mice.
Osteopontin deficiency promotes cartilaginous endplate degeneration by enhancing the NF-kappaB signaling to recruit macrophages and activate the NLRP3 inflammasome.
Title: Osteopontin deficiency promotes cartilaginous endplate degeneration by enhancing the NF-κB signaling to recruit macrophages and activate the NLRP3 inflammasome.
Osteopontin deletion attenuates cyst growth but exacerbates fibrosis in mice with cystic kidney disease.
Title: Osteopontin deletion attenuates cyst growth but exacerbates fibrosis in mice with cystic kidney disease.
A comprehensive single-cell RNA transcriptomic analysis identifies a unique SPP1+ macrophages subgroup in aging skeletal muscle.
Title: A comprehensive single-cell RNA transcriptomic analysis identifies a unique SPP1+ macrophages subgroup in aging skeletal muscle.
SOCS3 regulates pathological retinal angiogenesis through modulating SPP1 expression in microglia and macrophages.
Title: SOCS3 regulates pathological retinal angiogenesis through modulating SPP1 expression in microglia and macrophages.
Integrated bioinformatics analysis identified leucine rich repeat containing 15 and secreted phosphoprotein 1 as hub genes for calcific aortic valve disease and osteoarthritis.
Title: Integrated bioinformatics analysis identified leucine rich repeat containing 15 and secreted phosphoprotein 1 as hub genes for calcific aortic valve disease and osteoarthritis.
Osteopontin Promotes Angiogenesis in the Spinal Cord and Exerts a Protective Role Against Motor Function Impairment and Neuropathic Pain After Spinal Cord Injury.
Title: Osteopontin Promotes Angiogenesis in the Spinal Cord and Exerts a Protective Role Against Motor Function Impairment and Neuropathic Pain After Spinal Cord Injury.
Autophagy-dependent expression of osteopontin and its downstream Stat3 signaling contributes to lymphatic malformation progression to lymphangiosarcoma.
Title: Autophagy-dependent expression of osteopontin and its downstream Stat3 signaling contributes to lymphatic malformation progression to lymphangiosarcoma.
Tumor cell derived osteopontin and prostaglandin E2 synergistically promote the expansion of myeloid derived suppressor cells during the tumor immune escape phase.
Title: Tumor cell derived osteopontin and prostaglandin E2 synergistically promote the expansion of myeloid derived suppressor cells during the tumor immune escape phase.

Submit: New GeneRIF Correction See all GeneRIFs (439)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (11) 1 citation
Gene trapped (1)
QTL (2) 1 citation
Targeted (11) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Spp1 (e-PCR), detects polymorphism
- UniSTS:498486

Spp1 (e-PCR), detects polymorphism
- UniSTS:498509

UniSTS:280407 (e-PCR)
- UniSTS:280407

Spp1 (e-PCR), detects polymorphism
- UniSTS:280407

Spp1 (e-PCR), detects polymorphism
- UniSTS:470619

Pou5f1 (e-PCR), detects polymorphism
- UniSTS:144250

PMC316977P1 (e-PCR)
- UniSTS:273066

PMC316977P2 (e-PCR)
- UniSTS:273067

AA960535 (e-PCR)
- UniSTS:159407

D5Mit115 (e-PCR), detects polymorphism
- UniSTS:130236

D5Mit115.3 (e-PCR), detects polymorphism
- UniSTS:130237

Spp1 (e-PCR), detects polymorphism
- UniSTS:498409

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables cytokine activity	NAS Non-traceable Author Statement more info	PubMed
enables extracellular matrix binding	IBA Inferred from Biological aspect of Ancestor more info
enables extracellular matrix binding	IDA Inferred from Direct Assay more info	PubMed
enables integrin binding	IDA Inferred from Direct Assay more info	PubMed
enables integrin binding	ISO Inferred from Sequence Orthology more info
enables small molecule binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in androgen catabolic process	ISO Inferred from Sequence Orthology more info
involved_in bone mineralization	IDA Inferred from Direct Assay more info	PubMed
involved_in bone mineralization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in calcium ion homeostasis	IDA Inferred from Direct Assay more info	PubMed
involved_in cell adhesion	IBA Inferred from Biological aspect of Ancestor more info
involved_in cell adhesion	IEA Inferred from Electronic Annotation more info
involved_in cell adhesion	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cellular response to fluid shear stress	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within cellular response to leukemia inhibitory factor	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to testosterone stimulus	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within collecting duct development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within intracellular calcium ion homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within intracellular chloride ion homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within intracellular phosphate ion homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within intracellular sodium ion homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of apoptotic process	NAS Non-traceable Author Statement more info	PubMed
acts_upstream_of_or_within neutrophil chemotaxis	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within neutrophil chemotaxis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in osteoblast differentiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in osteoblast differentiation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of bone resorption	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of bone resorption	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of cell-substrate adhesion	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of estradiol secretion	ISO Inferred from Sequence Orthology more info
involved_in response to steroid hormone	ISO Inferred from Sequence Orthology more info
involved_in response to vitamin D	ISO Inferred from Sequence Orthology more info
involved_in signal transduction	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within urate biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
located_in apical part of cell	IDA Inferred from Direct Assay more info	PubMed
located_in cell projection	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in extracellular region	NAS Non-traceable Author Statement more info	PubMed
is_active_in extracellular space	IBA Inferred from Biological aspect of Ancestor more info
located_in extracellular space	IEA Inferred from Electronic Annotation more info
located_in extracellular space	ISO Inferred from Sequence Orthology more info
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info
located_in vesicle	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: osteopontin

Names: 44 kDa bone phosphoprotein; bone sialoprotein 1; calcium oxalate crystal growth inhibitor protein; early T-lymphocyte activation 1 protein; minopontin; osteopontin-like protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001204201.1 → NP_001191130.1 osteopontin isoform 1 precursor

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longest isoform (1).

Source sequence(s)

AC124106

UniProtKB/TrEMBL

Q3UBR1, Q3UZY3

Conserved Domains (1) summary

pfam00865
Location:38 → 311

Osteopontin; Osteopontin
NM_001204202.1 → NP_001191131.1 osteopontin isoform 2 precursor

See identical proteins and their annotated locations for NP_001191131.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks an exon in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.

Source sequence(s)

AC124106, AI882055, AK150845, AK165965

UniProtKB/TrEMBL

Q3UBR1, Q3UZY3

Conserved Domains (1) summary

pfam00865
Location:38 → 310

Osteopontin; Osteopontin
NM_001204203.1 → NP_001191132.1 osteopontin isoform 3 precursor

Status: VALIDATED

Description

Transcript Variant: This variant (3) differs in the 5' UTR and uses a downstream in-frame start codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus, compared to isoform 1.

Source sequence(s)

AC124106

Consensus CDS

CCDS57367.1

UniProtKB/TrEMBL

F8WIP8, Q3UBR1, Q3UZY3

Related

ENSMUSP00000084043.7, ENSMUST00000086833.13

Conserved Domains (1) summary

pfam00865
Location:22 → 295

Osteopontin; Osteopontin
NM_001204233.1 → NP_001191162.1 osteopontin isoform 4 precursor

See identical proteins and their annotated locations for NP_001191162.1

Status: VALIDATED

Description

Transcript Variant: This variant (5) differs in the 5' UTR, uses a downstream in-frame start codon and lacks an exon in the coding region, compared to variant 1. Variants 4 and 5 encode the same isoform (4), which is shorter than isoform 1.

Source sequence(s)

AC124106

Consensus CDS

CCDS19486.1

UniProtKB/Swiss-Prot

P10923, P19008, Q91VH4

UniProtKB/TrEMBL

Q3UBR1, Q3UZY3, Q547B5

Related

ENSMUSP00000108367.2, ENSMUST00000112747.2

Conserved Domains (1) summary

pfam00865
Location:22 → 294

Osteopontin; Osteopontin
NM_009263.3 → NP_033289.2 osteopontin isoform 4 precursor

See identical proteins and their annotated locations for NP_033289.2

Status: VALIDATED

Description

Transcript Variant: This variant (4) differs in the 5' UTR, uses a downstream in-frame start codon and lacks an exon in the coding region, compared to variant 1. Variants 4 and 5 encode the same isoform (4), which is shorter than isoform 1.

Source sequence(s)

AC124106, AI882055, AK160540

Consensus CDS

CCDS19486.1

UniProtKB/Swiss-Prot

P10923, P19008, Q91VH4

UniProtKB/TrEMBL

Q3UBR1, Q3UZY3, Q547B5

Related

ENSMUSP00000031243.9, ENSMUST00000031243.15

Conserved Domains (1) summary

pfam00865
Location:22 → 294

Osteopontin; Osteopontin