Uba3 ubiquitin-like modifier activating enzyme 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Uba3provided by MGI
Official Full Name: ubiquitin-like modifier activating enzyme 3provided by MGI
Primary source: MGI:MGI:1341217
See related: Ensembl:ENSMUSG00000030061 AllianceGenome:MGI:1341217
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Ube1c; A830034N06Rik
Summary: The protein encoded by this gene is the catalytic subunit of the enzyme that activates NEDD8, a ubiquitin-like molecule that binds to its target proteins through an enzymatic reaction analagous to ubiquitylation. Embryonic mice deficient for this protein die prior to implantation and display apoptosis of the inner cell mass. Trophoblastic cells cannot enter S phase, demonstrating that this gene is required for cell cycle progression during embryogenesis. Two pseudogenes have been found for this gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2014]
Expression: Broad expression in CNS E11.5 (RPKM 17.1), placenta adult (RPKM 15.3) and 24 other tissues See more
Orthologs: human all
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Genomic context

Location:: 6 D3; 6 44.9 cM

Exon count:: 19

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	6	NC_000072.7 (97160593..97182608, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	6	NC_000072.6 (97183632..97205647, complement)

Chromosome 6 - NC_000072.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Gene trapped (1)
Targeted (2) 1 citation
Transgenic (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AI848246 (e-PCR)
- UniSTS:165480

AW546539 (e-PCR)
- UniSTS:179424

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables NEDD8 activating enzyme activity	IBA Inferred from Biological aspect of Ancestor more info
enables NEDD8 activating enzyme activity	IDA Inferred from Direct Assay more info	PubMed
contributes_to NEDD8 activating enzyme activity	ISO Inferred from Sequence Orthology more info
enables NEDD8 activating enzyme activity	ISO Inferred from Sequence Orthology more info	PubMed
enables NEDD8 activating enzyme activity	TAS Traceable Author Statement more info	PubMed
enables identical protein binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables nuclear receptor binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein heterodimerization activity	IEA Inferred from Electronic Annotation more info
enables protein heterodimerization activity	ISO Inferred from Sequence Orthology more info
enables protein-containing complex binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within endomitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in protein neddylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein neddylation	IEA Inferred from Electronic Annotation more info
involved_in protein neddylation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
part_of protein-containing complex	IEA Inferred from Electronic Annotation more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: NEDD8-activating enzyme E1 catalytic subunit

Names: NEDD8-activating enzyme E1C; ubiquitin-activating enzyme 3; ubiquitin-activating enzyme E1C

NP_001104576.1

EC 6.2.1.64

NP_001288786.1

EC 6.2.1.64

NP_001288787.1

EC 6.2.1.64

NP_001288788.1

EC 6.2.1.64

NP_035796.2

EC 6.2.1.64

XP_011239592.1

EC 6.2.1.64

XP_030111175.1

EC 6.2.1.64

XP_030111176.1

EC 6.2.1.64

XP_036021915.1

EC 6.2.1.64

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001111106.2 → NP_001104576.1 NEDD8-activating enzyme E1 catalytic subunit isoform 2

See identical proteins and their annotated locations for NP_001104576.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. It encodes isoform 2 which is shorter compared to isoform 1.

Source sequence(s)

AK159381, AK166655, BY639644

Consensus CDS

CCDS51859.1

UniProtKB/TrEMBL

Q3TL72, Q66JT6

Related

ENSMUSP00000130954.2, ENSMUST00000164744.8

Conserved Domains (3) summary

cd01488
Location:57 → 354

Uba3_RUB; Ubiquitin activating enzyme (E1) subunit UBA3. UBA3 is part of the heterodimeric activating enzyme (E1), specific for the Rub family of ubiquitin-like proteins (Ubls). E1 enzymes are part of a conjugation cascade to attach Ub or Ubls, covalently to ...

pfam00899
Location:53 → 352

ThiF; ThiF family

pfam08825
Location:362 → 447

E2_bind; E2 binding domain
NM_001301857.1 → NP_001288786.1 NEDD8-activating enzyme E1 catalytic subunit isoform 3

See identical proteins and their annotated locations for NP_001288786.1

Status: REVIEWED

Description

Transcript Variant: This variant (3) uses an alternate splice site in the 5' coding region and uses a downstream start codon compared to variant 1. It encodes isoform 3 which has a shorter N-terminus compared to isoform 1.

Source sequence(s)

AK032514, AK159381, AK166655, BY639644

UniProtKB/TrEMBL

Q66JT6

Conserved Domains (2) summary

cd01488
Location:50 → 347

Uba3_RUB; Ubiquitin activating enzyme (E1) subunit UBA3. UBA3 is part of the heterodimeric activating enzyme (E1), specific for the Rub family of ubiquitin-like proteins (Ubls). E1 enzymes are part of a conjugation cascade to attach Ub or Ubls, covalently to ...

pfam08825
Location:355 → 440

E2_bind; E2 binding domain
NM_001301858.1 → NP_001288787.1 NEDD8-activating enzyme E1 catalytic subunit isoform 4

Status: REVIEWED

Description

Transcript Variant: This variant (4) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. It encodes an isoform (4) that is shorter compared to isoform 1.

Source sequence(s)

AK159381, AK166655, BC080776, BY639644

UniProtKB/TrEMBL

Q66JT6

Conserved Domains (3) summary

cd01488
Location:62 → 341

Uba3_RUB; Ubiquitin activating enzyme (E1) subunit UBA3. UBA3 is part of the heterodimeric activating enzyme (E1), specific for the Rub family of ubiquitin-like proteins (Ubls). E1 enzymes are part of a conjugation cascade to attach Ub or Ubls, covalently to ...

pfam00899
Location:62 → 339

ThiF; ThiF family

pfam08825
Location:349 → 434

E2_bind; E2 binding domain
NM_001301859.1 → NP_001288788.1 NEDD8-activating enzyme E1 catalytic subunit isoform 5

Status: REVIEWED

Description

Transcript Variant: This variant (5) lacks several exons and its 3' terminal exon extends past a splice site that is used in variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. It encodes isoform 5 which is shorter and has a distinct C-terminus, compared to isoform 1.

Source sequence(s)

AK014433, AK159381, CF928750

UniProtKB/TrEMBL

Q66JT6

Conserved Domains (2) summary

cd01488
Location:71 → 368

Uba3_RUB; Ubiquitin activating enzyme (E1) subunit UBA3. UBA3 is part of the heterodimeric activating enzyme (E1), specific for the Rub family of ubiquitin-like proteins (Ubls). E1 enzymes are part of a conjugation cascade to attach Ub or Ubls, covalently to ...

pfam00899
Location:67 → 366

ThiF; ThiF family
NM_011666.3 → NP_035796.2 NEDD8-activating enzyme E1 catalytic subunit isoform 1

See identical proteins and their annotated locations for NP_035796.2

Status: REVIEWED

Description

Transcript Variant: This variant (1) encodes the longest isoform (1).

Source sequence(s)

AK159381, AK166655, BY639644

Consensus CDS

CCDS51860.1

UniProtKB/Swiss-Prot

O88598, Q3TG68, Q8C878, Q9D6B7

UniProtKB/TrEMBL

Q66JT6

Related

ENSMUSP00000086701.6, ENSMUST00000089287.7

Conserved Domains (3) summary

cd01488
Location:71 → 368

Uba3_RUB; Ubiquitin activating enzyme (E1) subunit UBA3. UBA3 is part of the heterodimeric activating enzyme (E1), specific for the Rub family of ubiquitin-like proteins (Ubls). E1 enzymes are part of a conjugation cascade to attach Ub or Ubls, covalently to ...

pfam00899
Location:67 → 366

ThiF; ThiF family

pfam08825
Location:376 → 461

E2_bind; E2 binding domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000072.7 Reference GRCm39 C57BL/6J

Range

97160593..97182608 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030255316.2 → XP_030111176.1 NEDD8-activating enzyme E1 catalytic subunit isoform X2

UniProtKB/TrEMBL

Q66JT6

Conserved Domains (2) summary

cd01488
Location:48 → 327

Uba3_RUB; Ubiquitin activating enzyme (E1) subunit UBA3. UBA3 is part of the heterodimeric activating enzyme (E1), specific for the Rub family of ubiquitin-like proteins (Ubls). E1 enzymes are part of a conjugation cascade to attach Ub or Ubls, covalently to ...

pfam08825
Location:335 → 420

E2_bind; E2 binding domain
XM_030255315.2 → XP_030111175.1 NEDD8-activating enzyme E1 catalytic subunit isoform X1

UniProtKB/TrEMBL

Q66JT6

Conserved Domains (2) summary

cd01488
Location:50 → 347

Uba3_RUB; Ubiquitin activating enzyme (E1) subunit UBA3. UBA3 is part of the heterodimeric activating enzyme (E1), specific for the Rub family of ubiquitin-like proteins (Ubls). E1 enzymes are part of a conjugation cascade to attach Ub or Ubls, covalently to ...

pfam08825
Location:355 → 440

E2_bind; E2 binding domain
XM_036166022.1 → XP_036021915.1 NEDD8-activating enzyme E1 catalytic subunit isoform X1

UniProtKB/TrEMBL

Q66JT6

Conserved Domains (2) summary

cd01488
Location:50 → 347

Uba3_RUB; Ubiquitin activating enzyme (E1) subunit UBA3. UBA3 is part of the heterodimeric activating enzyme (E1), specific for the Rub family of ubiquitin-like proteins (Ubls). E1 enzymes are part of a conjugation cascade to attach Ub or Ubls, covalently to ...

pfam08825
Location:355 → 440

E2_bind; E2 binding domain
XM_011241290.4 → XP_011239592.1 NEDD8-activating enzyme E1 catalytic subunit isoform X1

See identical proteins and their annotated locations for XP_011239592.1

UniProtKB/TrEMBL

Q66JT6

Conserved Domains (2) summary

cd01488
Location:50 → 347

Uba3_RUB; Ubiquitin activating enzyme (E1) subunit UBA3. UBA3 is part of the heterodimeric activating enzyme (E1), specific for the Rub family of ubiquitin-like proteins (Ubls). E1 enzymes are part of a conjugation cascade to attach Ub or Ubls, covalently to ...

pfam08825
Location:355 → 440

E2_bind; E2 binding domain