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    Pou5f1 POU domain, class 5, transcription factor 1 [ Mus musculus (house mouse) ]

    Gene ID: 18999, updated on 2-Nov-2024

    Summary

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    Official Symbol
    Pou5f1provided by MGI
    Official Full Name
    POU domain, class 5, transcription factor 1provided by MGI
    Primary source
    MGI:MGI:101893
    See related
    Ensembl:ENSMUSG00000024406 AllianceGenome:MGI:101893
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    Oct3; Oct4; Otf3; Otf4; NF-A3; Oct-3; Oct-4; Otf-3; Otf-4; Otf3g; Oct3/4; Oct-3/4; Otf3-rs7
    Summary
    The protein encoded by this gene belongs to the POU domain family of transcription factors. POU domain transcription factors bind to a specific octamer DNA motif and regulate cell type-specific differentiation pathways. The encoded protein plays a key role in embryonic development and stem cell pluripotency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
    Expression
    Biased expression in testis adult (RPKM 4.0) and ovary adult (RPKM 1.5) See more
    Orthologs
    human all
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    Genomic context

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    See Pou5f1 in Genome Data Viewer
    Location:
    17 B1; 17 18.69 cM
    Exon count:
    6
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 17 NC_000083.7 (35816929..35821674)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 17 NC_000083.6 (35506032..35510777)

    Chromosome 17 - NC_000083.7Genomic Context describing neighboring genes Neighboring gene histocompatibility 2, Q region locus 10 Neighboring gene STARR-seq mESC enhancer starr_42453 Neighboring gene STARR-seq mESC enhancer starr_42454 Neighboring gene Pou5f1 5' regulatory region Neighboring gene predicted gene, 32362 Neighboring gene STARR-seq mESC enhancer starr_42459 Neighboring gene STARR-positive B cell enhancer mm9_chr17:35653747-35654048 Neighboring gene transcription factor 19 Neighboring gene coiled-coil alpha-helical rod protein 1

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. The Effect of Melatonin on OCT4 Expression and Granulosa Cell Growth in Female Mice. Fu B, et al. Reprod Sci, 2022 Oct. PMID 34735714
    2. Cruciform Formable Sequences within Pou5f1 Enhancer Are Indispensable for Mouse ES Cell Integrity. Yamamoto Y, et al. Int J Mol Sci, 2021 Mar 26. PMID 33810223, Free PMC Article
    3. Spermatogonial stem cell self-renewal requires OCT4, a factor downregulated during retinoic acid-induced differentiation. Dann CT, et al. Stem Cells, 2008 Nov. PMID 18719224
    4. Genome-wide functional analysis reveals factors needed at the transition steps of induced reprogramming. Yang CS, et al. Cell Rep, 2014 Jul 24. PMID 25043178, Free PMC Article
    5. In Vivo Expression of Reprogramming Factor OCT4 Ameliorates Myelination Deficits and Induces Striatal Neuroprotection in Huntington's Disease. Yu JH, et al. Genes (Basel), 2021 May 10. PMID 34068799, Free PMC Article

    See all (1000) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Oct4 redox sensitivity potentiates reprogramming and differentiation.
      Title: Oct4 redox sensitivity potentiates reprogramming and differentiation.
    2. Txnip regulates the Oct4-mediated pluripotency circuitry via metabolic changes upon differentiation.
      Title: Txnip regulates the Oct4-mediated pluripotency circuitry via metabolic changes upon differentiation.
    3. Regulatory Elements Outside Established Pou5f1 Gene Boundaries Are Required for Multilineage Differentiation of Embryonic Stem Cells.
      Title: Regulatory Elements Outside Established Pou5f1 Gene Boundaries Are Required for Multilineage Differentiation of Embryonic Stem Cells.
    4. Single-molecule tracking of Nanog and Oct4 in living mouse embryonic stem cells uncovers a feedback mechanism of pluripotency maintenance.
      Title: Single-molecule tracking of Nanog and Oct4 in living mouse embryonic stem cells uncovers a feedback mechanism of pluripotency maintenance.
    5. The homeodomain of Oct4 is a dimeric binder of methylated CpG elements.
      Title: The homeodomain of Oct4 is a dimeric binder of methylated CpG elements.
    6. Oct4 reduction contributes to testicular injury of unilateral testicular torsion in mice model and apoptotic death of Sertoli cells through mediating CIP2A expression.
      Title: Oct4 reduction contributes to testicular injury of unilateral testicular torsion in mice model and apoptotic death of Sertoli cells through mediating CIP2A expression.
    7. NARF is a hypoxia-induced coactivator for OCT4-mediated breast cancer stem cell specification.
      Title: NARF is a hypoxia-induced coactivator for OCT4-mediated breast cancer stem cell specification.
    8. OCT4 interprets and enhances nucleosome flexibility.
      Title: OCT4 interprets and enhances nucleosome flexibility.
    9. The Effect of Melatonin on OCT4 Expression and Granulosa Cell Growth in Female Mice.
      Title: The Effect of Melatonin on OCT4 Expression and Granulosa Cell Growth in Female Mice.
    10. Evolutionary origin of vertebrate OCT4/POU5 functions in supporting pluripotency.
      Title: Evolutionary origin of vertebrate OCT4/POU5 functions in supporting pluripotency.
    Submit: New GeneRIF Correction See all GeneRIFs (329)

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (1) 
    • Targeted (11)  1 citation

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables DNA-binding transcription activator activity, RNA polymerase II-specific IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA-binding transcription factor activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA-binding transcription factor activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables DNA-binding transcription factor activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables DNA-binding transcription factor activity, RNA polymerase II-specific IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables DNA-binding transcription factor activity, RNA polymerase II-specific IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA-binding transcription factor activity, RNA polymerase II-specific IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables DNA-binding transcription factor binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables DNA-binding transcription repressor activity, RNA polymerase II-specific ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables HMG box domain binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables HMG box domain binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables POU domain binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables POU domain binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables RNA polymerase II cis-regulatory region sequence-specific DNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables RNA polymerase II cis-regulatory region sequence-specific DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables RNA polymerase II cis-regulatory region sequence-specific DNA binding IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    enables RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables RNA polymerase II-specific DNA-binding transcription factor binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables RNA polymerase II-specific DNA-binding transcription factor binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables chromatin DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables chromatin binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables cis-regulatory region sequence-specific DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables cytokine binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables lysine-acetylated histone binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables miRNA binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables sequence-specific DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables sequence-specific DNA binding ISO
    Inferred from Sequence Orthology
    more info
    		 PubMed 
    enables sequence-specific double-stranded DNA binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables transcription cis-regulatory region binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables transcription cis-regulatory region binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables ubiquitin protein ligase binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables ubiquitin protein ligase binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    Process Evidence Code Pubs
    involved_in blastocyst development IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within blastocyst growth IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within cell fate commitment IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within cellular response to leukemia inhibitory factor IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within ectodermal cell fate commitment IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within endodermal cell fate commitment IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within endodermal cell fate specification ISO
    Inferred from Sequence Orthology
    more info
    		 PubMed 
    involved_in gene expression IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within germ-line stem cell population maintenance IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within mesodermal cell fate commitment IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within negative regulation of DNA-templated transcription IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within negative regulation of calcium ion-dependent exocytosis IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within negative regulation of cell differentiation IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in negative regulation of gene expression IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within negative regulation of transcription by RNA polymerase II IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within positive regulation of DNA-templated transcription IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within positive regulation of gene expression IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in positive regulation of gene expression IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in positive regulation of transcription by RNA polymerase II IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of transcription by RNA polymerase II IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II ISO
    Inferred from Sequence Orthology
    more info
    		 PubMed 
    involved_in positive regulation of transcription initiation by RNA polymerase II IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within regulation of DNA-templated transcription IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in regulation of DNA-templated transcription ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in regulation of asymmetric cell division IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    involved_in regulation of transcription by RNA polymerase II IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in regulation of transcription by RNA polymerase II IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in response to benzoic acid IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within response to retinoic acid IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within somatic stem cell population maintenance IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within stem cell differentiation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in stem cell differentiation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in stem cell population maintenance IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within transcription by RNA polymerase II IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of trophectodermal cell differentiation IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within trophectodermal cell differentiation IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within trophectodermal cell fate commitment IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in chromatin IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in chromatin IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    located_in cytoplasm ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in cytosol ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in female germ cell nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in germ cell nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in male germ cell nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in mitochondrion ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in nucleolus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleoplasm ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in nucleus IC
    Inferred by Curator
    more info
    		 PubMed 
    is_active_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    located_in nucleus ISO
    Inferred from Sequence Orthology
    more info
    		  
    part_of transcription regulator complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of transcription regulator complex ISO
    Inferred from Sequence Orthology
    more info
    		  
    part_of transcription repressor complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    POU domain, class 5, transcription factor 1
    Names
    octamer-binding protein 3
    octamer-binding protein 4
    octamer-binding transcription factor 3
    octamer-binding transcription factor 4 alternative

    NCBI Reference Sequences (RefSeq)

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    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001252452.1 → NP_001239381.1  POU domain, class 5, transcription factor 1 isoform 2

      See identical proteins and their annotated locations for NP_001239381.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region and uses a downstream, in-frame start codon, compared to variant 1. the encoded isoform (2) has a shorter N-terminus, compared to isoform 1.
      Source sequence(s)
      AB375270, AW536651, BY390845
      UniProtKB/TrEMBL
      A0A2I6EDI9, A0A2I6EDJ6
      Conserved Domains (2) summary
      smart00352
      Location:1 → 74
      POU; Found in Pit-Oct-Unc transcription factors
      pfam00046
      Location:95 → 148
      Homeobox; Homeobox domain

    2. NM_013633.3 → NP_038661.2  POU domain, class 5, transcription factor 1 isoform 1

      See identical proteins and their annotated locations for NP_038661.2

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
      Source sequence(s)
      AK145321, BY390845, CU467494
      Consensus CDS
      CCDS37600.1
      UniProtKB/Swiss-Prot
      P20263, Q63843
      Related
      ENSMUSP00000025271.10, ENSMUST00000025271.17
      Conserved Domains (2) summary
      smart00352
      Location:131 → 205
      POU; Found in Pit-Oct-Unc transcription factors
      pfam00046
      Location:226 → 279
      Homeobox; Homeobox domain

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000083.7 Reference GRCm39 C57BL/6J

      Range
      35816929..35821674
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P20263.1 GenPept UniProtKB/Swiss-Prot:P20263

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

    Chemical Information
    Molecular Biology Databases
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