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    Ano2 anoctamin 2 [ Mus musculus (house mouse) ]

    Gene ID: 243634, updated on 2-Nov-2024

    Summary

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    Official Symbol
    Ano2provided by MGI
    Official Full Name
    anoctamin 2provided by MGI
    Primary source
    MGI:MGI:2387214
    See related
    Ensembl:ENSMUSG00000038115 AllianceGenome:MGI:2387214
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    Tmem16b
    Summary
    Enables identical protein binding activity and intracellularly calcium-gated chloride channel activity. Acts upstream of or within chloride transport and establishment of localization in cell. Located in neuron projection; non-motile cilium; and plasma membrane. Is active in glutamatergic synapse and presynaptic membrane. Is expressed in dorsal root ganglion; epidermis; medulla oblongata basal plate mantle layer; olfactory epithelium; and spinal cord mantle layer. Orthologous to human ANO2 (anoctamin 2). [provided by Alliance of Genome Resources, Nov 2024]
    Expression
    Biased expression in testis adult (RPKM 1.4), placenta adult (RPKM 0.4) and 8 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Ano2 in Genome Data Viewer
    Location:
    6 F3; 6 59.58 cM
    Exon count:
    28
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 6 NC_000072.7 (125667382..126017115)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 6 NC_000072.6 (125690419..126040133)

    Chromosome 6 - NC_000072.7Genomic Context describing neighboring genes Neighboring gene STARR-positive B cell enhancer ABC_E2804 Neighboring gene STARR-seq mESC enhancer starr_17384 Neighboring gene predicted gene, 33144 Neighboring gene Von Willebrand factor Neighboring gene predicted gene, 38898 Neighboring gene predicted gene, 33202 Neighboring gene predicted gene, 24947 Neighboring gene STARR-seq mESC enhancer starr_17386 Neighboring gene STARR-seq mESC enhancer starr_17388 Neighboring gene STARR-seq mESC enhancer starr_17389 Neighboring gene neurotrophin 3 Neighboring gene STARR-seq mESC enhancer starr_17391 Neighboring gene STARR-seq mESC enhancer starr_17392 Neighboring gene predicted gene 10415

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Paradoxical electro-olfactogram responses in TMEM16B knock-out mice. Guarneri G, et al. Chem Senses, 2023 Jan 1. PMID 36744918, Free PMC Article
    2. Calcium concentration jumps reveal dynamic ion selectivity of calcium-activated chloride currents in mouse olfactory sensory neurons and TMEM16b-transfected HEK 293T cells. Sagheddu C, et al. J Physiol, 2010 Nov 1. PMID 20837642, Free PMC Article
    3. Tmem16b is specifically expressed in the cilia of olfactory sensory neurons. Rasche S, et al. Chem Senses, 2010 Mar. PMID 20100788
    4. Interactions between permeation and gating in the TMEM16B/anoctamin2 calcium-activated chloride channel. Betto G, et al. J Gen Physiol, 2014 Jun. PMID 24863931, Free PMC Article
    5. Anoctamin2 (TMEM16B) forms the Ca(2+)-activated Cl(-) channel in the retinal pigment epithelium. Keckeis S, et al. Exp Eye Res, 2017 Jan. PMID 27940219

    See all (53) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Paradoxical electro-olfactogram responses in TMEM16B knock-out mice.
      Title: Paradoxical electro-olfactogram responses in TMEM16B knock-out mice.
    2. TMEM16b regulates action potential firing in lateral septum and aggression in male mice.
      Title: TMEM16B Calcium-Activated Chloride Channels Regulate Action Potential Firing in Lateral Septum and Aggression in Male Mice.
    3. Ano2/TMEM16B is a Ca2+-activated chloride channel in vagal afferents of nodose neurons and a major determinant of CCK-induced satiety, body weight control, and energy expenditure, making it a potential therapeutic target in obesity.
      Title: TMEM16B determines cholecystokinin sensitivity of intestinal vagal afferents of nodose neurons.
    4. Here the authors report that Ano2 knockout mice exhibit impaired anxiety-related behaviors and context-independent fear memory, thus implicating TMEM16B in anxiety modulation.
      Title: TMEM16B regulates anxiety-related behavior and GABAergic neuronal signaling in the central lateral amygdala.
    5. Study provides the first in vivo evidence toward an alternative or additional role for Ano2 in the olfactory transduction cascade, where it may serve as a feedback mechanism to clamp olfactory receptor neurons spike output.
      Title: Calcium-activated chloride channels clamp odor-evoked spike activity in olfactory receptor neurons.
    6. Results of behavioral studies obtained from Ano2 (-/-) mice, a mouse line that was previously shown to lack this particular mode of ionic plasticity. The animals display motor coordination deficits that constitute a condition of mild ataxia. Moreover, motor learning is severely impaired in Ano2 (-/-) mice, suggesting cerebellar dysfunction.
      Title: Impaired Motor Coordination and Learning in Mice Lacking Anoctamin 2 Calcium-Gated Chloride Channels.
    7. Results suggest that the anoctamin 2-mediated amplification mechanism enables mice to track weak, unfamiliar olfactory cues.
      Title: Tracking of unfamiliar odors is facilitated by signal amplification through anoctamin 2 chloride channels in mouse olfactory receptor neurons.
    8. TMEM16B controls AP firing and ensures correct glomerular targeting of olfactory sensory neurons s expressing I7.
      Title: The Ca2+-activated Cl- channel TMEM16B regulates action potential firing and axonal targeting in olfactory sensory neurons.
    9. Loss of TMEM16B expression resulted in the absence of Calcium-activated Chloride channels in inferior Olive neurons, leading to markedly diminished action potential firing of IO neurons. TMEM16B knockout mice. Moreover, exhibited severe cerebellar motor learning deficits.
      Title: Inferior Olivary TMEM16B Mediates Cerebellar Motor Learning.
    10. The siRNA knock-down suggests that Ano2 contributes to Ca(2+)-dependent chloride conductance in the RPE.
      Title: Anoctamin2 (TMEM16B) forms the Ca(2+)-activated Cl(-) channel in the retinal pigment epithelium.
    Submit: New GeneRIF Correction See all GeneRIFs (26)

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Clone Names

    • MGC38715

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables chloride channel activity TAS
    Traceable Author Statement
    more info
    		  
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables intracellularly calcium-gated chloride channel activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables intracellularly calcium-gated chloride channel activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables intracellularly calcium-gated chloride channel activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables intracellularly calcium-gated chloride channel activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    NOT enables phospholipid scramblase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein dimerization activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein dimerization activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    Process Evidence Code Pubs
    involved_in chloride transmembrane transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in chloride transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within chloride transport IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within chloride transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within establishment of localization in cell IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within establishment of localization in cell IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of chloride channel complex IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in glutamatergic synapse IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in neuron projection IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in non-motile cilium IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleoplasm ISO
    Inferred from Sequence Orthology
    more info
    		  
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in plasma membrane TAS
    Traceable Author Statement
    more info
    		  
    is_active_in presynaptic membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    anoctamin-2

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001364562.1 → NP_001351491.1  anoctamin-2 isoform 2

      Status: VALIDATED

      Source sequence(s)
      KC164759
      UniProtKB/TrEMBL
      M1GTU4, M1H0B0
      Conserved Domains (2) summary
      pfam04547
      Location:351 → 936
      Anoctamin; Calcium-activated chloride channel
      pfam16178
      Location:165 → 348
      Anoct_dimer; Dimerisation domain of Ca+-activated chloride-channel, anoctamin

    2. NM_001364563.1 → NP_001351492.1  anoctamin-2 isoform 3

      Status: VALIDATED

      Source sequence(s)
      KC164761
      UniProtKB/TrEMBL
      M1GNA0, M1GRW0
      Conserved Domains (2) summary
      pfam04547
      Location:289 → 874
      Anoctamin; Calcium-activated chloride channel
      pfam16178
      Location:103 → 286
      Anoct_dimer; Dimerisation domain of Ca+-activated chloride-channel, anoctamin

    3. NM_153589.2 → NP_705817.2  anoctamin-2 isoform 1

      See identical proteins and their annotated locations for NP_705817.2

      Status: VALIDATED

      Source sequence(s)
      AK044763, BC033409, BC060368
      Consensus CDS
      CCDS39643.2
      UniProtKB/Swiss-Prot
      C4N788, Q8C8N6, Q8CFW1
      UniProtKB/TrEMBL
      M1GTU4
      Related
      ENSMUSP00000125303.2, ENSMUST00000160496.8
      Conserved Domains (2) summary
      pfam04547
      Location:351 → 940
      Anoctamin; Calcium-activated chloride channel
      pfam16178
      Location:91 → 348
      Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000072.7 Reference GRCm39 C57BL/6J

      Range
      125667382..126017115
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_006506117.4 → XP_006506180.1  anoctamin-2 isoform X2

      UniProtKB/TrEMBL
      M1GNA0
      Conserved Domains (2) summary
      pfam04547
      Location:289 → 878
      Anoctamin; Calcium-activated chloride channel
      pfam16178
      Location:29 → 286
      Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin

    2. XM_006506115.3 → XP_006506178.1  anoctamin-2 isoform X1

      UniProtKB/TrEMBL
      M1GNA0
      Conserved Domains (2) summary
      pfam04547
      Location:306 → 895
      Anoctamin; Calcium-activated chloride channel
      pfam16178
      Location:46 → 303
      Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin

    3. XM_006506119.3 → XP_006506182.1  anoctamin-2 isoform X3

      See identical proteins and their annotated locations for XP_006506182.1

      UniProtKB/TrEMBL
      M1GNA0
      Conserved Domains (2) summary
      pfam04547
      Location:262 → 851
      Anoctamin; Calcium-activated chloride channel
      pfam16178
      Location:2 → 259
      Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q8CFW1.2 GenPept UniProtKB/Swiss-Prot:Q8CFW1

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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