Rad18 RAD18 E3 ubiquitin protein ligase [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Rad18provided by MGI
Official Full Name: RAD18 E3 ubiquitin protein ligaseprovided by MGI
Primary source: MGI:MGI:1890476
See related: Ensembl:ENSMUSG00000030254 AllianceGenome:MGI:1890476
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Rad18sc; 2810024C04Rik
Summary: Predicted to enable several functions, including Y-form DNA binding activity; identical protein binding activity; and ubiquitin protein ligase binding activity. Acts upstream of or within several processes, including negative regulation of DNA recombination; response to UV; and spermatogenesis. Located in XY body; chromatin; and male germ cell nucleus. Is expressed in several structures, including adrenal medulla; dorsal root ganglion; testis; trunk mesenchyme derived from neural crest; and urinary system. Orthologous to human RAD18 (RAD18 E3 ubiquitin protein ligase). [provided by Alliance of Genome Resources, Oct 2024]
Expression: Broad expression in testis adult (RPKM 6.5), liver E14 (RPKM 4.9) and 19 other tissues See more
Orthologs: human all
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Genomic context

Location:: 6 E3; 6 52.42 cM

Exon count:: 15

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	6	NC_000072.7 (112596812..112673650, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	6	NC_000072.6 (112619851..112696691, complement)

Chromosome 6 - NC_000072.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Genome-wide CRISPR screen identified Rad18 as a determinant of doxorubicin sensitivity in osteosarcoma.
Title: Genome-wide CRISPR screen identified Rad18 as a determinant of doxorubicin sensitivity in osteosarcoma.
these results demonstrate that the tumor-suppressive role of DNA polymerase Eta in UV-treated skin is Rad18 independent
Title: Differential Roles of Rad18 and Chk2 in Genome Maintenance and Skin Carcinogenesis Following UV Exposure.
Somatic hypermutation of immunoglobulin genes (SHM) in Rad18 KO mice was similar to wild-type mice, suggesting that Rad18 is dispensable for SHM.
Title: Somatic hypermutation of immunoglobulin genes in Rad18 knockout mice.
Rad18 confers DNA damage tolerance and tumor-suppression in a physiological setting.
Title: Rad18 confers hematopoietic progenitor cell DNA damage tolerance independently of the Fanconi Anemia pathway in vivo.
REV1 promote PCNA monoubiquitylation after UV radiation through interacting with ubiquitylated RAD18.
Title: REV1 promotes PCNA monoubiquitylation through interacting with ubiquitylated RAD18.
RAD18 can function as a mediator for DNA damage response signals to activate the G2/M checkpoint in order to maintain genome integrity and cell survival after IR exposure.
Title: RAD18 activates the G2/M checkpoint through DNA damage signaling to maintain genome integrity after ionizing radiation exposure.
repairs meiotic double-strand DNA breaks and and maintains meiotic sex chromosome inactivation
Title: Meiotic functions of RAD18.
RAD18 is intrinsically required for the long-term maintenance of spermatogenesis.
Title: Rad18 is required for long-term maintenance of spermatogenesis in mouse testes.
Rad18 suppresses viral infection in a non-specific fashion, probably by targeting incoming DNA.
Title: Effect of DNA repair protein Rad18 on viral infection.
RAD18 contributes to maintenance of genomic stability through postreplication repair
Title: Enhanced genomic instability and defective postreplication repair in RAD18 knockout mouse embryonic stem cells.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Targeted (2) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables Y-form DNA binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables polyubiquitin modification-dependent protein binding	ISO Inferred from Sequence Orthology more info
enables polyubiquitin modification-dependent protein binding	ISS Inferred from Sequence or Structural Similarity more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-containing complex binding	ISO Inferred from Sequence Orthology more info
enables single-stranded DNA binding	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within DNA damage response	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within DNA damage response	ISO Inferred from Sequence Orthology more info	PubMed
involved_in DNA damage response	ISO Inferred from Sequence Orthology more info
involved_in DNA damage response	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within negative regulation of DNA recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of chromosome segregation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of chromosome segregation	ISS Inferred from Sequence or Structural Similarity more info
involved_in postreplication repair	IBA Inferred from Biological aspect of Ancestor more info
involved_in postreplication repair	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within protein autoubiquitination	ISO Inferred from Sequence Orthology more info	PubMed
involved_in protein monoubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein monoubiquitination	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within response to UV	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within spermatogenesis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of Rad6-Rad18 complex	IBA Inferred from Biological aspect of Ancestor more info
located_in XY body	IDA Inferred from Direct Assay more info	PubMed
located_in centrosome	ISO Inferred from Sequence Orthology more info
located_in centrosome	ISS Inferred from Sequence or Structural Similarity more info
located_in chromatin	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in male germ cell nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nuclear body	ISO Inferred from Sequence Orthology more info
located_in nuclear inclusion body	ISO Inferred from Sequence Orthology more info
located_in nuclear inclusion body	ISS Inferred from Sequence or Structural Similarity more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info	PubMed
located_in replication fork	ISO Inferred from Sequence Orthology more info
located_in site of double-strand break	ISO Inferred from Sequence Orthology more info
located_in site of double-strand break	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: E3 ubiquitin-protein ligase RAD18

Names: RAD18 homolog; RING-type E3 ubiquitin transferase RAD18; mRAD18Sc; post-replication repair protein RAD18SC; postreplication repair protein RAD18

NP_001161202.1

EC 2.3.2.27

NP_001368860.1

EC 2.3.2.27

NP_001368861.1

EC 2.3.2.27

NP_001368862.1

EC 2.3.2.27

NP_067360.2

EC 2.3.2.27

XP_006506497.1

EC 2.3.2.27

XP_006506500.1

EC 2.3.2.27

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001167730.1 → NP_001161202.1 E3 ubiquitin-protein ligase RAD18 isoform 1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AC157095, AK028505, AK030043

Consensus CDS

CCDS51870.1

UniProtKB/TrEMBL

E9Q392, Q8CDH2

Related

ENSMUSP00000070619.6, ENSMUST00000068487.12

Conserved Domains (4) summary

smart00734
Location:201 → 224

ZnF_Rad18; Rad18-like CCHC zinc finger

cd00162
Location:25 → 63

RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...

TIGR00599
Location:13 → 310

rad18; DNA repair protein rad18

pfam02037
Location:248 → 282

SAP; SAP domain
NM_001381931.1 → NP_001368860.1 E3 ubiquitin-protein ligase RAD18 isoform 3

Status: VALIDATED

Source sequence(s)

AC153591, AC157095

UniProtKB/TrEMBL

Q8CDH2

Related

ENSMUSP00000108807.2, ENSMUST00000113182.8

Conserved Domains (1) summary

TIGR00599
Location:13 → 363

rad18; DNA repair protein rad18
NM_001381932.1 → NP_001368861.1 E3 ubiquitin-protein ligase RAD18 isoform 4

Status: VALIDATED

Source sequence(s)

AC153591, AC157095

UniProtKB/TrEMBL

Q8CDH2

Conserved Domains (1) summary

TIGR00599
Location:13 → 310

rad18; DNA repair protein rad18
NM_001381933.1 → NP_001368862.1 E3 ubiquitin-protein ligase RAD18 isoform 5

Status: VALIDATED

Source sequence(s)

AC153591, AC157095

Conserved Domains (1) summary

TIGR00599
Location:58 → 231

rad18; DNA repair protein rad18
NM_021385.2 → NP_067360.2 E3 ubiquitin-protein ligase RAD18 isoform 2

See identical proteins and their annotated locations for NP_067360.2

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks two alternate in-frame exons compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.

Source sequence(s)

AC157095, AK012795

Consensus CDS

CCDS39589.1

UniProtKB/Swiss-Prot

Q9CZB8, Q9QXK2

UniProtKB/TrEMBL

Q8CDH2

Related

ENSMUSP00000076341.5, ENSMUST00000077088.11

Conserved Domains (4) summary

smart00734
Location:201 → 224

ZnF_Rad18; Rad18-like CCHC zinc finger

cd00162
Location:25 → 63

RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...

TIGR00599
Location:13 → 363

rad18; DNA repair protein rad18

pfam02037
Location:248 → 282

SAP; SAP domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000072.7 Reference GRCm39 C57BL/6J

Range

112596812..112673650 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006506437.5 → XP_006506500.1 E3 ubiquitin-protein ligase RAD18 isoform X2

Related

ENSMUSP00000108805.2, ENSMUST00000113180.8

Conserved Domains (1) summary

TIGR00599
Location:13 → 310

rad18; DNA repair protein rad18
XM_006506434.5 → XP_006506497.1 E3 ubiquitin-protein ligase RAD18 isoform X1

UniProtKB/TrEMBL

Q8CDH2

Conserved Domains (1) summary

TIGR00599
Location:13 → 310

rad18; DNA repair protein rad18