Pknox1 Pbx/knotted 1 homeobox [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Pknox1provided by MGI
Official Full Name: Pbx/knotted 1 homeoboxprovided by MGI
Primary source: MGI:MGI:1201409
See related: Ensembl:ENSMUSG00000006705 AllianceGenome:MGI:1201409
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: PREP1; D17Wsu76e
Summary: Enables DNA-binding transcription factor activity, RNA polymerase II-specific; RNA polymerase II cis-regulatory region sequence-specific DNA binding activity; and chromatin binding activity. Acts upstream of or within several processes, including angiogenesis; hemopoiesis; and positive regulation of transcription by RNA polymerase II. Located in cytoplasm and nucleus. Part of transcription regulator complex. Is expressed in several structures, including alimentary system; branchial arch; genitourinary system; nervous system; and sensory organ. Orthologous to human PKNOX1 (PBX/knotted 1 homeobox 1). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in thymus adult (RPKM 12.1), testis adult (RPKM 10.4) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 17 B1; 17 16.78 cM

Exon count:: 12

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	17	NC_000083.7 (31783697..31826665)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	17	NC_000083.6 (31564722..31607693)

Chromosome 17 - NC_000083.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Interleukin 6 reduces vascular smooth muscle cell apoptosis via Prep1 and is associated with aging.
Title: Interleukin 6 reduces vascular smooth muscle cell apoptosis via Prep1 and is associated with aging.
Prep1, complexing with p160, decreases PGC-1alpha and stimulates angiogenesis.
Title: Prep1 regulates angiogenesis through a PGC-1α-mediated mechanism.
Prep1 DR expands the DNA binding landscape of C/EBPb (CCAAT enhancer binding protein b) without affecting its expression or activation. The data indicate that Prep1 normally acts by restricting DNA binding of transcription factors to adipogenic enhancers, in particular C/EBPb.
Title: Prep1 prevents premature adipogenesis of mesenchymal progenitors.
We conclude that Prep1 is required for normal adult mammary gland development, in particular at its branching morphogenesis step. By binding Snail2, Prep1 protects it from the proteasomal degradation.
Title: Prep1 (pKnox1) transcription factor contributes to pubertal mammary gland branching morphogenesis.
a mechanism by which the DNA binding-independent activity of Sp2 potentiates genomic loading of Pbx1:Prep1 and Nf-y to composite motifs present in many promoters of highly expressed genes
Title: Transcription factor Sp2 potentiates binding of the TALE homeoproteins Pbx1:Prep1 and the histone-fold domain protein Nf-y to composite genomic sites.
Prep1 deficiency alters olfactory morpho-functional integrity and olfaction-mediated eating behavior by affecting BDNF-TrkB signaling.
Title: Prep1 Deficiency Affects Olfactory Perception and Feeding Behavior by Impairing BDNF-TrkB Mediated Neurotrophic Signaling.
Prep1 silencing reduces inflammatory response and increases insulin sensitivity in adipose tissue.
Title: Prep1 deficiency improves metabolic response in white adipose tissue.
results indicate that PKnox1 is a critical regulator of maintenance and subsequent differentiation of the c-Kit+ stage of spermatogonia in the adult testes
Title: Germ cell-intrinsic requirement for the homeodomain transcription factor PKnox1/Prep1 in adult spermatogenesis.
However, Prep1 doesn't affect gluconeogenesis in the absence of insulin. Also, we observed that nuclear content of gluconeogenic transcription factor FOXO1 was greatly reduced in Prep1 knockout hepatocytes. These findings suggest that Prep1 may control hepatic insulin sensitivity by targeting FOXO1 nuclear stability.
Title: The transcription factor Prep1 controls hepatic insulin sensitivity and gluconeogenesis by targeting nuclear localization of FOXO1.
Study found that knockdown of Prep1 gene expression affects adipogenic differentiation and results in significant increase in the insulin-sensitive glucose carrier Glut4 gene expression
Title: Transcriptional control of insulin-sensitive glucose carrier Glut4 expression in adipose tissue cells.

Submit: New GeneRIF Correction See all GeneRIFs (36)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Gene trapped (4)
Targeted (7) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Pknox1 (e-PCR), detects polymorphism
- UniSTS:234018

Pknox1 (e-PCR), detects polymorphism
- UniSTS:259129

Pknox1 (e-PCR), detects polymorphism
- UniSTS:259646

RH125898 (e-PCR)
- UniSTS:142177

51J6-T7 (e-PCR), detects polymorphism
- UniSTS:140960

RH124906 (e-PCR)
- UniSTS:161728

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IGI Inferred from Genetic Interaction more info	PubMed
enables chromatin binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables sequence-specific double-stranded DNA binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within T cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in angiogenesis	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within angiogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within camera-type eye development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within erythrocyte differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within hemopoiesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of DNA-templated transcription	TAS Traceable Author Statement more info	PubMed
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of transcription by RNA polymerase II	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	TAS Traceable Author Statement more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
part_of transcription regulator complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: homeobox protein PKNOX1

Names: PBX/knotted homeobox 1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001411456.1 → NP_001398385.1 homeobox protein PKNOX1 isoform 1

Status: VALIDATED

Source sequence(s)

AC166172, AC166575

UniProtKB/Swiss-Prot

O70477, Q7TT01
NM_001411457.1 → NP_001398386.1 homeobox protein PKNOX1 isoform 1

Status: VALIDATED

Source sequence(s)

AC166172, AC166575

UniProtKB/Swiss-Prot

O70477, Q7TT01
NM_001411458.1 → NP_001398387.1 homeobox protein PKNOX1 isoform 1

Status: VALIDATED

Source sequence(s)

AC166172, AC166575

UniProtKB/Swiss-Prot

O70477, Q7TT01
NM_001411459.1 → NP_001398388.1 homeobox protein PKNOX1 isoform 1

Status: VALIDATED

Source sequence(s)

AC166172, AC166575

UniProtKB/Swiss-Prot

O70477, Q7TT01
NM_001411460.1 → NP_001398389.1 homeobox protein PKNOX1 isoform 2

Status: VALIDATED

Source sequence(s)

AC166172, AC166575

UniProtKB/TrEMBL

A0A494B9U0

Related

ENSMUSP00000157748.2, ENSMUST00000237248.2
NM_001411461.1 → NP_001398390.1 homeobox protein PKNOX1 isoform 2

Status: VALIDATED

Source sequence(s)

AC166172, AC166575

UniProtKB/TrEMBL

A0A494B9U0
NM_001411462.1 → NP_001398391.1 homeobox protein PKNOX1 isoform 2

Status: VALIDATED

Source sequence(s)

AC166172, AC166575

UniProtKB/TrEMBL

A0A494B9U0
NM_001411463.1 → NP_001398392.1 homeobox protein PKNOX1 isoform 2

Status: VALIDATED

Source sequence(s)

AC166172, AC166575

UniProtKB/TrEMBL

A0A494B9U0
NM_001411464.1 → NP_001398393.1 homeobox protein PKNOX1 isoform 3

Status: VALIDATED

Source sequence(s)

AC166172, AC166575
NM_001411465.1 → NP_001398394.1 homeobox protein PKNOX1 isoform 3

Status: VALIDATED

Source sequence(s)

AC166172, AC166575
NM_001411466.1 → NP_001398395.1 homeobox protein PKNOX1 isoform 4

Status: VALIDATED

Source sequence(s)

AC166172, AC166575
NM_001411467.1 → NP_001398396.1 homeobox protein PKNOX1 isoform 5

Status: VALIDATED

Source sequence(s)

AC166172, AC166575
NM_001411468.1 → NP_001398397.1 homeobox protein PKNOX1 isoform 6

Status: VALIDATED

Source sequence(s)

AC166172, AC166575

Related

ENSMUSP00000157491.2, ENSMUST00000237038.2
NM_001411469.1 → NP_001398398.1 homeobox protein PKNOX1 isoform 6

Status: VALIDATED

Source sequence(s)

AC166172, AC166575
NM_001411864.1 → NP_001398793.1 homeobox protein PKNOX1 isoform 1

Status: VALIDATED

Source sequence(s)

AC166172, AC166575

UniProtKB/Swiss-Prot

O70477, Q7TT01

Related

ENSMUSP00000134852.2, ENSMUST00000175806.9
NM_016670.4 → NP_057879.2 homeobox protein PKNOX1 isoform 1

See identical proteins and their annotated locations for NP_057879.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the functional protein.

Source sequence(s)

AC166172, AC166575

Consensus CDS

CCDS28608.1

UniProtKB/Swiss-Prot

O70477, Q7TT01

Related

ENSMUSP00000094966.4, ENSMUST00000097352.11

Conserved Domains (2) summary

pfam05920
Location:277 → 316

Homeobox_KN; Homeobox KN domain

pfam16493
Location:80 → 165

Meis_PKNOX_N; N-terminal of Homeobox Meis and PKNOX1

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000083.7 Reference GRCm39 C57BL/6J

Range

31783697..31826665

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036160407.1 → XP_036016300.1 homeobox protein PKNOX1 isoform X4

Conserved Domains (2) summary

pfam05920
Location:274 → 313

Homeobox_KN; Homeobox KN domain

pfam16493
Location:80 → 165

Meis_PKNOX_N; N-terminal of Homeobox Meis and PKNOX1

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

NR_027493.1: Suppressed sequence

Description

NR_027493.1: This RefSeq was removed because it is now thought that this transcript does encode a protein.