Chrna3 cholinergic receptor, nicotinic, alpha polypeptide 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Chrna3provided by MGI
Official Full Name: cholinergic receptor, nicotinic, alpha polypeptide 3provided by MGI
Primary source: MGI:MGI:87887
See related: Ensembl:ENSMUSG00000032303 AllianceGenome:MGI:87887
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: (a)3; Acra3; Acra-3; A730007P14Rik
Summary: Enables acetylcholine-gated monoatomic cation-selective channel activity. Acts upstream of or within several processes, including activation of transmembrane receptor protein tyrosine kinase activity; behavioral response to nicotine; and chemical synaptic transmission. Part of acetylcholine-gated channel complex. Is active in cholinergic synapse and postsynaptic specialization membrane. Is expressed in several structures, including central nervous system; gut; myenteric nerve plexus; paravertebral ganglion; and retina. Human ortholog(s) of this gene implicated in several diseases, including alcohol dependence; chronic obstructive pulmonary disease; cocaine dependence; lung cancer (multiple); and nicotine dependence. Orthologous to human CHRNA3 (cholinergic receptor nicotinic alpha 3 subunit). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in adrenal adult (RPKM 6.2), whole brain E14.5 (RPKM 4.2) and 11 other tissues See more
Orthologs: human all
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Genomic context

Location:: 9 B; 9 29.84 cM

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	9	NC_000075.7 (54917401..54933846, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	9	NC_000075.6 (55010117..55026562, complement)

Chromosome 9 - NC_000075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Abundant extrasynaptic expression of alpha3beta4-containing nicotinic acetylcholine receptors in the medial habenula-interpeduncular nucleus pathway in mice.
Title: Abundant extrasynaptic expression of α3β4-containing nicotinic acetylcholine receptors in the medial habenula-interpeduncular nucleus pathway in mice.
Differential tyrosine and serine/threonine phosphorylation/dephosphorylation pathways regulate the expression of alpha7 versus alpha3beta4 nicotinic receptor subtypes in mouse hippocampal neurons.
Title: Differential tyrosine and serine/threonine phosphorylation/dephosphorylation pathways regulate the expression of α7 versus α3β4 nicotinic receptor subtypes in mouse hippocampal neurons.
Bidirectional sex-dependent regulation of alpha6 and beta3 nicotinic acetylcholine receptors by protein kinase Cepsilon.
Title: Bidirectional sex-dependent regulation of α6 and β3 nicotinic acetylcholine receptors by protein kinase Cε.
alpha3* Nicotinic Acetylcholine Receptors in the Habenula-Interpeduncular Nucleus Circuit Regulate Nicotine Intake.
Title: α3* Nicotinic Acetylcholine Receptors in the Habenula-Interpeduncular Nucleus Circuit Regulate Nicotine Intake.
Regulation of nicotinic acetylcholine receptor alpha3 subtype in adipose tissue dysfunction
Title: Regulation of nicotinic acetylcholine receptor α3 subtype in adipose tissue dysfunction.
Mechanoinsensitive ''silent'' nociceptors are characterized by the expression of the nicotinic acetylcholine receptor subunit alpha-3 (CHRNA3); the mechanically gated ion channel PIEZO2 mediates NGF-induced mechanosensitivity in these neurons.
Title: Functional and Molecular Characterization of Mechanoinsensitive "Silent" Nociceptors.
neuroinflammation is sufficient to provoke the decrease of a7 and a4b2 nAChRs, Ab42 accumulation and memory impairment in mice and a7(1-208) nAChR-specific antibodies can cause inflammation of the brain with symptoms typical for Alzheimer disease
Title: α7 Nicotinic acetylcholine receptor-specific antibody induces inflammation and amyloid β42 accumulation in the mouse brain to impair memory.
The alpha3beta4* nicotinic ACh receptor subtype mediates physical dependence to morphine
Title: The α3β4* nicotinic ACh receptor subtype mediates physical dependence to morphine: mouse and human studies.
The present findings suggest that its thymic production and/or release are under cholinergic control involving nAChR containing the alpha3-subunit.
Title: Terminally differentiated epithelial cells of the thymic medulla and skin express nicotinic acetylcholine receptor subunit α 3.
Downregulation of nAChR subunit and PSD-93 expression after cavernous nerve injury, or even manipulation, could interrupt synaptic transmission within the MPG and thus contribute to the loss of neural control of urogenital organs after pelvic surgeries.
Title: Decrease in neuronal nicotinic acetylcholine receptor subunit and PSD-93 transcript levels in the male mouse MPG after cavernous nerve injury or explant culture.

Submit: New GeneRIF Correction See all GeneRIFs (20)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (5)
Targeted (4) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

NoName (e-PCR)
- UniSTS:491584

BE108721 (e-PCR)
- UniSTS:247872

Chrna3 (e-PCR), detects polymorphism
- UniSTS:256423

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables acetylcholine binding	ISO Inferred from Sequence Orthology more info
contributes_to acetylcholine receptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables acetylcholine receptor activity	ISO Inferred from Sequence Orthology more info
enables acetylcholine-gated monoatomic cation-selective channel activity	IBA Inferred from Biological aspect of Ancestor more info
enables acetylcholine-gated monoatomic cation-selective channel activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables acetylcholine-gated monoatomic cation-selective channel activity	ISO Inferred from Sequence Orthology more info
enables heterocyclic compound binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-containing complex binding	ISO Inferred from Sequence Orthology more info
enables transmembrane signaling receptor activity	IEA Inferred from Electronic Annotation more info
enables transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential	IDA Inferred from Direct Assay more info	PubMed
enables transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in acetylcholine receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in acetylcholine receptor signaling pathway	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within activation of transmembrane receptor protein tyrosine kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within behavioral response to nicotine	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in behavioral response to nicotine	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within excitatory postsynaptic potential	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within locomotory behavior	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in membrane depolarization	IBA Inferred from Biological aspect of Ancestor more info
involved_in monoatomic ion transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in monoatomic ion transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in nervous system development	ISO Inferred from Sequence Orthology more info
involved_in presynaptic modulation of chemical synaptic transmission	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of acetylcholine secretion, neurotransmission	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of dendrite morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of membrane potential	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of muscle contraction	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of smooth muscle contraction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to acetylcholine	ISO Inferred from Sequence Orthology more info
involved_in response to nicotine	IBA Inferred from Biological aspect of Ancestor more info
involved_in response to xenobiotic stimulus	ISO Inferred from Sequence Orthology more info
involved_in signal transduction	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within synaptic transmission involved in micturition	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in synaptic transmission involved in micturition	ISO Inferred from Sequence Orthology more info
involved_in synaptic transmission, cholinergic	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within synaptic transmission, cholinergic	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	IEA Inferred from Electronic Annotation more info
part_of acetylcholine-gated channel complex	IBA Inferred from Biological aspect of Ancestor more info
part_of acetylcholine-gated channel complex	IPI Inferred from Physical Interaction more info	PubMed
part_of acetylcholine-gated channel complex	ISO Inferred from Sequence Orthology more info
is_active_in cholinergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in cholinergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in cholinergic synapse	ISO Inferred from Sequence Orthology more info
located_in dendrite	ISO Inferred from Sequence Orthology more info
is_active_in dopaminergic synapse	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
is_active_in neuron projection	IBA Inferred from Biological aspect of Ancestor more info
located_in neuronal cell body	ISO Inferred from Sequence Orthology more info
located_in nuclear speck	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane raft	ISO Inferred from Sequence Orthology more info
located_in postsynaptic membrane	IC Inferred by Curator more info	PubMed
is_active_in postsynaptic specialization membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynaptic specialization membrane	ISO Inferred from Sequence Orthology more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info
is_active_in synapse	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: neuronal acetylcholine receptor subunit alpha-3

Names: alpha 3; neuronal nicotinic acetylcholine receptor, alpha 3 subunit

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_145129.3 → NP_660111.3 neuronal acetylcholine receptor subunit alpha-3 precursor

Status: VALIDATED

Source sequence(s)

AC156832, AC161264

Consensus CDS

CCDS23199.2

UniProtKB/Swiss-Prot

Q8BV44, Q8R4G9

UniProtKB/TrEMBL

Q8VHH6

Related

ENSMUSP00000034851.6, ENSMUST00000034851.7

Conserved Domains (2) summary

TIGR00860
Location:24 → 490

LIC; Cation transporter family protein

cd19016
Location:29 → 235

LGIC_ECD_nAChR_A3; extracellular domain of nicotinic acetylcholine receptor subunit alpha 3 (CHRNA3)