Msrb1 methionine sulfoxide reductase B1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Msrb1provided by MGI
Official Full Name: methionine sulfoxide reductase B1provided by MGI
Primary source: MGI:MGI:1351642
See related: Ensembl:ENSMUSG00000075705 AllianceGenome:MGI:1351642
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: SELX; SelR; Sepr; Sepx1; D17Wsu82e
Summary: The protein encoded by this gene belongs to the methionine-R-sulfoxide reductase B (MsrB) family. Members of this family function as repair enzymes that protect proteins from oxidative stress by catalyzing the reduction of methionine-R-sulfoxides to methionines. This protein is highly expressed in liver and kidney, and is localized to the nucleus and cytosol. It is the only member of the MsrB family that is a selenoprotein, containing a selenocysteine (Sec) residue at its active site. It also has the highest methionine-R-sulfoxide reductase activity compared to other members containing cysteine in place of Sec. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Oct 2016]
Expression: Ubiquitous expression in liver adult (RPKM 166.6), bladder adult (RPKM 66.9) and 25 other tissues See more
Orthologs: human all
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Genomic context

Location:: 17 A3.3; 17 12.53 cM

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	17	NC_000083.7 (24955567..24961752)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	17	NC_000083.6 (24736593..24742778)

Chromosome 17 - NC_000083.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

MsrB1-regulated GAPDH oxidation plays programmatic roles in shaping metabolic and inflammatory signatures during macrophage activation.
Title: MsrB1-regulated GAPDH oxidation plays programmatic roles in shaping metabolic and inflammatory signatures during macrophage activation.
these results suggest that MsrB1 is essential for mice spatial learning and LTP/LTD induction, and the MsrB1 related redox homeostasis may be involved in regulating the phosphorylation of CaMKIIs.
Title: Loss of MsrB1 perturbs spatial learning and long-term potentiation/long-term depression in mice.
Findings suggest that MsrB1 controls immune responses by promoting anti-inflammatory cytokine expression in macrophages. MsrB1-dependent reduction of oxidized methionine in proteins may be a heretofore unrecognized regulatory event underlying immunity and inflammatory disease.
Title: Selenoprotein MsrB1 promotes anti-inflammatory cytokine gene expression in macrophages and controls immune response in vivo.
Collectively, the results of this study suggest that selenoprotein MsrB1 plays a protective role against APAP-induced hepatotoxicity via its antioxidative function.
Title: Selenoprotein MsrB1 deficiency exacerbates acetaminophen-induced hepatotoxicity via increased oxidative damage.
MsrB1 deficiency does not increase high-fat diet-induced insulin resistance in mice.
Title: Methionine sulfoxide reductase B1 deficiency does not increase high-fat diet-induced insulin resistance in mice.
study identified the regulatory role of MsrB1 as a Mical antagonist in orchestrating actin dynamics and macrophage function
Title: MsrB1 and MICALs regulate actin assembly and macrophage function via reversible stereoselective methionine oxidation.
MsrB1/Trx complex was studied in attempt to understand MsrB1 reduction mechanism. Using NMR data, molecular mechanics, protein docking & molecular dynamics simulations, found that intermediate MsrB1/Trx complex is stabilized by interprotein beta-layer.
Title: Structural insights into interaction between mammalian methionine sulfoxide reductase B1 and thioredoxin.
Analyses of fruit flies that do not express selenoproteins or express the mouse selenoprotein, methionine sulfoxide reductase B1, reveal a role of selenoproteins in stress resistance.
Title: Analyses of fruit flies that do not express selenoproteins or express the mouse selenoprotein, methionine sulfoxide reductase B1, reveal a role of selenoproteins in stress resistance.
Insights into function, catalytic mechanism, and fold evolution of selenoprotein methionine sulfoxide reductase B1 through structural analysis.
Title: Insights into function, catalytic mechanism, and fold evolution of selenoprotein methionine sulfoxide reductase B1 through structural analysis.
Study characterized unexpected diversity of protein and mRNA forms of mammalian selenoprotein MsrB1.
Title: Diversity of protein and mRNA forms of mammalian methionine sulfoxide reductase B1 due to intronization and protein processing.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Gene trapped (1) 1 citation
Targeted (1) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables L-methionine-(R)-S-oxide reductase activity	IEA Inferred from Electronic Annotation more info
enables actin binding	IDA Inferred from Direct Assay more info	PubMed
enables peptide-methionine (R)-S-oxide reductase activity	IBA Inferred from Biological aspect of Ancestor more info
enables peptide-methionine (R)-S-oxide reductase activity	IDA Inferred from Direct Assay more info	PubMed
enables zinc ion binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in actin filament polymerization	IDA Inferred from Direct Assay more info	PubMed
involved_in innate immune response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein repair	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within protein repair	IDA Inferred from Direct Assay more info	PubMed
involved_in protein repair	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
colocalizes_with actin cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: methionine-R-sulfoxide reductase B1

Names: selenoprotein R; selenoprotein X 1

NP_001333597.1

EC 1.8.4.12

EC 1.8.4.14

NP_038787.1

EC 1.8.4.12

EC 1.8.4.14

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001346668.1 → NP_001333597.1 methionine-R-sulfoxide reductase B1

Status: REVIEWED

Description

Transcript Variant: This variant (2) is a 4-exon transcript that is alternatively spliced at the 3' end compared to variant 1. Variants 1 and 2 encode the same protein.

Source sequence(s)

AK003112, AK007404, BI133579, BY034747

Consensus CDS

CCDS28495.1

UniProtKB/Swiss-Prot

Q545U8, Q9JLC3

UniProtKB/TrEMBL

B2RUH3

Related

ENSMUSP00000099300.7, ENSMUST00000101800.7
NM_013759.3 → NP_038787.1 methionine-R-sulfoxide reductase B1

See identical proteins and their annotated locations for NP_038787.1

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the predominant 5-exon transcript. Variants 1 and 2 encode the same protein.

Source sequence(s)

BC090646, BI133579, BY034747

Consensus CDS

CCDS28495.1

UniProtKB/Swiss-Prot

Q545U8, Q9JLC3

UniProtKB/TrEMBL

B2RUH3

Related

ENSMUSP00000110917.3, ENSMUST00000115262.9

Conserved Domains (1) summary

cl15841
Location:7 → 108

SelR; SelR domain