Cyb5r3 cytochrome b5 reductase 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Cyb5r3provided by MGI
Official Full Name: cytochrome b5 reductase 3provided by MGI
Primary source: MGI:MGI:94893
See related: Ensembl:ENSMUSG00000018042 AllianceGenome:MGI:94893
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: B5R; Dia1; Dia-1; WU:Cyb5r3; 0610016L08Rik; 2500002N19Rik
Summary: Predicted to enable cytochrome-b5 reductase activity, acting on NAD(P)H and nucleotide binding activity. Predicted to contribute to nitrite reductase (NO-forming) activity. Predicted to be involved in nitric oxide biosynthetic process. Located in mitochondrial inner membrane. Is expressed in several structures, including alimentary system; brain; cardiovascular system; genitourinary system; and hemolymphoid system gland. Human ortholog(s) of this gene implicated in breast cancer and methemoglobinemia. Orthologous to human CYB5R3 (cytochrome b5 reductase 3). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Broad expression in duodenum adult (RPKM 409.4), adrenal adult (RPKM 270.7) and 23 other tissues See more
Orthologs: human all
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Genomic context

Location:: 15 E1; 15 39.4 cM

Exon count:: 12

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	15	NC_000081.7 (83037696..83060641, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	15	NC_000081.6 (83153495..83176440, complement)

Chromosome 15 - NC_000081.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

CYB5R3 overexpression exhibits sexual dimorphism: Mitochondrial and metabolic adaptations in transgenic female mice during calorie restriction.
Title: CYB5R3 overexpression exhibits sexual dimorphism: Mitochondrial and metabolic adaptations in transgenic female mice during calorie restriction.
Apolipoprotein O modulates cholesterol metabolism via NRF2/CYB5R3 independent of LDL receptor.
Title: Apolipoprotein O modulates cholesterol metabolism via NRF2/CYB5R3 independent of LDL receptor.
Cyb5r3 activation rescues secondary failure to sulfonylurea but not beta-cell dedifferentiation.
Title: Cyb5r3 activation rescues secondary failure to sulfonylurea but not β-cell dedifferentiation.
CYB5R3 functions as a tumor suppressor by inducing ER stress-mediated apoptosis in lung cancer cells via the PERK-ATF4 and IRE1alpha-JNK pathways.
Title: CYB5R3 functions as a tumor suppressor by inducing ER stress-mediated apoptosis in lung cancer cells via the PERK-ATF4 and IRE1α-JNK pathways.
Sex-specific metabolic adaptations in transgenic mice overexpressing cytochrome b5 reductase-3.
Title: Sex-specific metabolic adaptations in transgenic mice overexpressing cytochrome b(5) reductase-3.
Cyb5r3-based mechanism and reversal of secondary failure to sulfonylurea in diabetes.
Title: Cyb5r3-based mechanism and reversal of secondary failure to sulfonylurea in diabetes.
The UFM1 system regulates ER-phagy through the ufmylation of CYB5R3.
Title: The UFM1 system regulates ER-phagy through the ufmylation of CYB5R3.
Smooth muscle cell CYB5R3 preserves cardiac and vascular function under chronic hypoxic stress.
Title: Smooth muscle cell CYB5R3 preserves cardiac and vascular function under chronic hypoxic stress.
Cooperation between CYB5R3 and NOX4 via coenzyme Q mitigates endothelial inflammation.
Title: Cooperation between CYB5R3 and NOX4 via coenzyme Q mitigates endothelial inflammation.
Mitochondrial adaptations in liver and skeletal muscle to pro-longevity nutritional and genetic interventions: the crosstalk between calorie restriction and CYB5R3 overexpression in transgenic mice.
Title: Mitochondrial adaptations in liver and skeletal muscle to pro-longevity nutritional and genetic interventions: the crosstalk between calorie restriction and CYB5R3 overexpression in transgenic mice.

Submit: New GeneRIF Correction See all GeneRIFs (19)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Targeted (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

GFM109b (e-PCR)
- UniSTS:528670

Cyb5r3 (e-PCR), detects polymorphism
- UniSTS:546711

RH127129 (e-PCR)
- UniSTS:209123

Cyb5r3 (e-PCR), detects polymorphism
- UniSTS:499178

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ADP binding	ISO Inferred from Sequence Orthology more info
enables AMP binding	ISO Inferred from Sequence Orthology more info
enables FAD binding	IBA Inferred from Biological aspect of Ancestor more info
enables FAD binding	ISO Inferred from Sequence Orthology more info
enables FAD binding	ISS Inferred from Sequence or Structural Similarity more info
enables NAD binding	ISO Inferred from Sequence Orthology more info
enables cytochrome-b5 reductase activity, acting on NAD(P)H	ISO Inferred from Sequence Orthology more info
enables cytochrome-b5 reductase activity, acting on NAD(P)H	ISS Inferred from Sequence or Structural Similarity more info
enables flavin adenine dinucleotide binding	ISO Inferred from Sequence Orthology more info
contributes_to nitrite reductase (NO-forming) activity	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in cholesterol biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in nitric oxide biosynthetic process	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
is_active_in endoplasmic reticulum membrane	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum membrane	ISO Inferred from Sequence Orthology more info
is_active_in endoplasmic reticulum membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in endoplasmic reticulum membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in lipid droplet	ISO Inferred from Sequence Orthology more info
located_in mitochondrial inner membrane	HDA more info	PubMed
located_in mitochondrial membrane	ISO Inferred from Sequence Orthology more info
located_in mitochondrial outer membrane	ISO Inferred from Sequence Orthology more info
located_in mitochondrial outer membrane	ISS Inferred from Sequence or Structural Similarity more info
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
part_of nitric-oxide synthase complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: NADH-cytochrome b5 reductase 3

Names: diaphorase 1 (NADH)

NP_084063.1

EC 1.6.2.2

XP_006520345.2

EC 1.6.2.2

XP_006520346.1

EC 1.6.2.2

XP_030104133.1

EC 1.6.2.2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_029787.3 → NP_084063.1 NADH-cytochrome b5 reductase 3

See identical proteins and their annotated locations for NP_084063.1

Status: VALIDATED

Source sequence(s)

AI839369, BC043074

Consensus CDS

CCDS27698.1

UniProtKB/Swiss-Prot

Q9DCN2

Related

ENSMUSP00000018186.10, ENSMUST00000018186.16

Conserved Domains (1) summary

PLN02252
Location:14 → 301

PLN02252; nitrate reductase [NADPH]

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000081.7 Reference GRCm39 C57BL/6J

Range

83037696..83060641 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030248273.1 → XP_030104133.1 NADH-cytochrome b5 reductase 3 isoform X2

UniProtKB/Swiss-Prot

Q9DCN2

Conserved Domains (1) summary

PLN02252
Location:14 → 301

PLN02252; nitrate reductase [NADPH]
XM_006520282.3 → XP_006520345.2 NADH-cytochrome b5 reductase 3 isoform X1

Conserved Domains (1) summary

PLN02252
Location:33 → 320

PLN02252; nitrate reductase [NADPH]
XM_006520283.5 → XP_006520346.1 NADH-cytochrome b5 reductase 3 isoform X3

UniProtKB/Swiss-Prot

Q9DCN2

Related

ENSMUSP00000124062.3, ENSMUST00000162834.3

Conserved Domains (1) summary

PLN02252
Location:15 → 278

PLN02252; nitrate reductase [NADPH]