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    Crisp2 cysteine-rich secretory protein 2 [ Mus musculus (house mouse) ]

    Gene ID: 22024, updated on 2-Nov-2024

    Summary

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    Official Symbol
    Crisp2provided by MGI
    Official Full Name
    cysteine-rich secretory protein 2provided by MGI
    Primary source
    MGI:MGI:98815
    See related
    Ensembl:ENSMUSG00000023930 AllianceGenome:MGI:98815
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    Tpx1; Tpx-1; GAPDL5; CRISP-2
    Summary
    Predicted to be involved in cell-cell adhesion. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Orthologous to human CRISP2 (cysteine rich secretory protein 2). [provided by Alliance of Genome Resources, Nov 2024]
    Expression
    Restricted expression toward testis adult (RPKM 326.9) See more
    Orthologs
    human all
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    Genomic context

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    See Crisp2 in Genome Data Viewer
    Location:
    17 B2; 17 19.54 cM
    Exon count:
    10
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 17 NC_000083.7 (41075625..41105037, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 17 NC_000083.6 (40764734..40794146, complement)

    Chromosome 17 - NC_000083.7Genomic Context describing neighboring genes Neighboring gene exocrine gland secreted peptide 3 Neighboring gene STARR-seq mESC enhancer starr_42547 Neighboring gene exocrine gland secreted peptide 1 Neighboring gene Rhesus blood group-associated A glycoprotein Neighboring gene STARR-seq mESC enhancer starr_42548 Neighboring gene STARR-seq mESC enhancer starr_42549 Neighboring gene cytochrome P450, family 2, subfamily ac, polypeptide 1, pseudogene

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. CRISP2 Is a Regulator of Multiple Aspects of Sperm Function and Male Fertility. Lim S, et al. Endocrinology, 2019 Apr 1. PMID 30759213
    2. Random cloning of genes from mouse chromosome 17. Kasahara M, et al. Proc Natl Acad Sci U S A, 1987 May. PMID 3472212, Free PMC Article
    3. Evidence for the involvement of testicular protein CRISP2 in mouse sperm-egg fusion. Busso D, et al. Biol Reprod, 2007 Apr. PMID 17202389
    4. The cysteine-rich secretory protein domain of Tpx-1 is related to ion channel toxins and regulates ryanodine receptor Ca2+ signaling. Gibbs GM, et al. J Biol Chem, 2006 Feb 17. PMID 16339766
    5. The mouse male germ cell-specific gene Tpx-1: molecular structure, mode of expression in spermatogenesis, and sequence similarity to two non-mammalian genes. Mizuki N, et al. Mamm Genome, 1992. PMID 1638086

    See all (37) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. we revealed a role for CRISP2 in establishing the ability of sperm to undergo the acrosome reaction and in establishing a normal flagellum waveform. Crisp2-deficient sperm possess a stiff midpiece and are thus unable to manifest the rapid form of progressive motility seen in wild type sperm. As a consequence, Crisp2-deficient males are subfertile.
      Title: CRISP2 Is a Regulator of Multiple Aspects of Sperm Function and Male Fertility.
    2. Crisp2 genetic defects in sperm from humans and mice result in male infertility.
      Title: Fertilization defects in sperm from Cysteine-rich secretory protein 2 (Crisp2) knockout mice: implications for fertility disorders.
    3. expression and localization of SHTAP in testis and sperm raise the possibility that the SHTAP-CRISP2 complex has a potential role in sperm formation and/or function.
      Title: A novel protein, sperm head and tail associated protein (SHTAP), interacts with cysteine-rich secretory protein 2 (CRISP2) during spermatogenesis in the mouse.
    4. These data raise the possibility that CRISP2 is a MAP3K11-modifying protein or, alternatively, that MAP3K11 acts to phosphorylate CRISP2 during acrosome development.
      Title: Cysteine-rich secretory protein 2 binds to mitogen-activated protein kinase kinase kinase 11 in mouse sperm.
    5. Evidence for a functional role of CRISP2 in fertilization.
      Title: Evidence for the involvement of testicular protein CRISP2 in mouse sperm-egg fusion.
    6. Tpx-1 Crisp domain on its own can regulate ion channel activity and provide compelling evidence for a role for Tpx-1 in the regulation of Ca(2+) fluxes observed during sperm capacitation.
      Title: The cysteine-rich secretory protein domain of Tpx-1 is related to ion channel toxins and regulates ryanodine receptor Ca2+ signaling.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (3) 
    • Targeted (3)  1 citation

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Process Evidence Code Pubs
    involved_in cell-cell adhesion IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in cell-cell adhesion ISO
    Inferred from Sequence Orthology
    more info
    		  
    Component Evidence Code Pubs
    located_in extracellular region IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in extracellular space IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  

    General protein information

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    Preferred Names
    cysteine-rich secretory protein 2
    Names
    testis specific gene 1
    testis-specific protein TPX-1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001204071.1 → NP_001191000.1  cysteine-rich secretory protein 2 precursor

      See identical proteins and their annotated locations for NP_001191000.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein.
      Source sequence(s)
      AC135084, BC049615, CJ046590
      Consensus CDS
      CCDS28786.1
      UniProtKB/Swiss-Prot
      P16563
      Conserved Domains (2) summary
      cd05383
      Location:36 → 171
      SCP_CRISP; SCP_CRISP: SCP-like extracellular protein domain, CRISP-like sub-family. The wider family of SCP containing proteins includes plant pathogenesis-related protein 1 (PR-1), CRISPs, mammalian cysteine-rich secretory proteins, which combine SCP with a ...
      pfam08562
      Location:189 → 243
      Crisp; Crisp

    2. NM_009420.2 → NP_033446.1  cysteine-rich secretory protein 2 precursor

      See identical proteins and their annotated locations for NP_033446.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.
      Source sequence(s)
      AC135084, BC049615
      Consensus CDS
      CCDS28786.1
      UniProtKB/Swiss-Prot
      P16563
      Related
      ENSMUSP00000024724.8, ENSMUST00000024724.14
      Conserved Domains (2) summary
      cd05383
      Location:36 → 171
      SCP_CRISP; SCP_CRISP: SCP-like extracellular protein domain, CRISP-like sub-family. The wider family of SCP containing proteins includes plant pathogenesis-related protein 1 (PR-1), CRISPs, mammalian cysteine-rich secretory proteins, which combine SCP with a ...
      pfam08562
      Location:189 → 243
      Crisp; Crisp

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000083.7 Reference GRCm39 C57BL/6J

      Range
      41075625..41105037 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_006524036.3 → XP_006524099.1  cysteine-rich secretory protein 2 isoform X1

      Conserved Domains (2) summary
      pfam08562
      Location:128 → 182
      Crisp
      cl00133
      Location:1 → 111
      CAP; CAP (cysteine-rich secretory proteins, antigen 5, and pathogenesis-related 1 proteins) domain family

    2. XM_030249644.1 → XP_030105504.1  cysteine-rich secretory protein 2 isoform X1

      Conserved Domains (2) summary
      pfam08562
      Location:128 → 182
      Crisp
      cl00133
      Location:1 → 111
      CAP; CAP (cysteine-rich secretory proteins, antigen 5, and pathogenesis-related 1 proteins) domain family
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P16563.1 GenPept UniProtKB/Swiss-Prot:P16563

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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