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    Ldhb lactate dehydrogenase B [ Mus musculus (house mouse) ]

    Gene ID: 16832, updated on 28-Oct-2024

    Summary

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    Official Symbol
    Ldhbprovided by MGI
    Official Full Name
    lactate dehydrogenase Bprovided by MGI
    Primary source
    MGI:MGI:96763
    See related
    Ensembl:ENSMUSG00000030246 AllianceGenome:MGI:96763
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    Ldh2; H-Ldh; LDH-B; LDH-H; Ldh-2
    Summary
    This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have also been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes 1 and 19. [provided by RefSeq, Feb 2016]
    Expression
    Broad expression in kidney adult (RPKM 644.8), heart adult (RPKM 618.4) and 18 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Ldhb in Genome Data Viewer
    Location:
    6 G2; 6 74.17 cM
    Exon count:
    8
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 6 NC_000072.7 (142435975..142453683, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 6 NC_000072.6 (142490249..142507957, complement)

    Chromosome 6 - NC_000072.7Genomic Context describing neighboring genes Neighboring gene spexin hormone Neighboring gene glycogen synthase 2 Neighboring gene CapStarr-seq enhancer MGSCv37_chr6:142438685-142438868 Neighboring gene STARR-seq mESC enhancer starr_17706 Neighboring gene STARR-seq mESC enhancer starr_17707 Neighboring gene predicted gene, 38919 Neighboring gene predicted gene, 46979

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Lactate dehydrogenase polymorphism in Mus musculus L. and Mus spretus Lataste. Britton-Davidian J, et al. Experientia, 1978 Sep 15. PMID 720506
    2. The cDNA and protein sequences of mouse lactate dehydrogenase B. Molecular evolution of vertebrate lactate dehydrogenase genes A (muscle), B (heart) and C (testis). Hiraoka BY, et al. Eur J Biochem, 1990 Apr 30. PMID 2338075
    3. Frontal-subcortical protein expression following prenatal exposure to maternal inflammation. Deng MY, et al. PLoS One, 2011 Feb 10. PMID 21347362, Free PMC Article
    4. Secretome profile of mouse oocytes after activation using mass spectrum. Peng Q, et al. J Assist Reprod Genet, 2012 Aug. PMID 22573034, Free PMC Article
    5. Early diagnosis of radiodermatitis using lactate dehydrogenase isozymes in hairless mice (SKH1-hr). Cho SK, et al. Lab Anim Res, 2012 Dec. PMID 23326284, Free PMC Article

    See all (85) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Integrated analysis of single cell-RNA sequencing and Mendelian randomization identifies lactate dehydrogenase B as a target of melatonin in ischemic stroke.
      Title: Integrated analysis of single cell-RNA sequencing and Mendelian randomization identifies lactate dehydrogenase B as a target of melatonin in ischemic stroke.
    2. LDHB contributes to the regulation of lactate levels and basal insulin secretion in human pancreatic beta cells.
      Title: LDHB contributes to the regulation of lactate levels and basal insulin secretion in human pancreatic β cells.
    3. Lactate dehydrogenase B regulates macrophage metabolism in the tumor microenvironment.
      Title: Lactate dehydrogenase B regulates macrophage metabolism in the tumor microenvironment.
    4. Expression of lactate dehydrogenase A and B isoforms in the mouse kidney.
      Title: Expression of lactate dehydrogenase A and B isoforms in the mouse kidney.
    5. these results identified a previously unrecognized Ldhb-driven alteration in muscle mitochondrial function and suggested a mechanism for the adaptive metabolic response induced by exercise training.
      Title: Exercise Inducible Lactate Dehydrogenase B Regulates Mitochondrial Function in Skeletal Muscle.
    6. Findings suggest that RANKL protein-induced lactate dehydrogenase (LDH) activation stimulates glycolytic and mitochondrial respiratory metabolism via transcription factor NFATc1 signaling.
      Title: Accelerated Lactate Dehydrogenase Activity Potentiates Osteoclastogenesis via NFATc1 Signaling.
    7. drs regulates glucose metabolism via LDH-B. Downregulating drs may contribute to the Warburg effect, which is closely associated with malignant progression of cancer cells.
      Title: The drs tumor suppressor regulates glucose metabolism via lactate dehydrogenase-B.
    8. Exercise-induced changes in tumour LDH-B and MCT1 expression are modulated by oestrogen-related receptor alpha in breast cancer-bearing BALB/c mice
      Title: Exercise-induced changes in tumour LDH-B and MCT1 expression are modulated by oestrogen-related receptor alpha in breast cancer-bearing BALB/c mice.
    9. Data show that PI3K/Akt signalling pathway and lactate dehydrogenase (LDH) play a crucial role in increase of cardiac ATP-binding cassette transporter sub-family C member 9 (SUR2A) induced by in vivo exposure to 18% oxygen.
      Title: Mild hypoxia in vivo regulates cardioprotective SUR2A: A role for Akt and LDH.
    10. PGC-1alpha in skeletal muscle drives the expression of lactate dehydrogenase (LDH) B in an estrogen-related receptor-alpha-dependent manner.
      Title: Skeletal muscle PGC-1α controls whole-body lactate homeostasis through estrogen-related receptor α-dependent activation of LDH B and repression of LDH A.
    Submit: New GeneRIF Correction See all GeneRIFs (15)

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (5) 
    • Targeted (3) 

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables L-lactate dehydrogenase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables L-lactate dehydrogenase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables L-lactate dehydrogenase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables NAD binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables identical protein binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables kinase binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables lactate dehydrogenase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    Process Evidence Code Pubs
    involved_in NAD metabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within lactate biosynthetic process from pyruvate IC
    Inferred by Curator
    more info
    		  
    involved_in lactate metabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in lactate metabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in pyruvate metabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in pyruvate metabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytosol ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in membrane raft ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in mitochondrial inner membrane ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in mitochondrial inner membrane ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    located_in mitochondrion HDA PubMed 
    is_active_in mitochondrion IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in myelin sheath HDA PubMed 
    part_of oxidoreductase complex ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    L-lactate dehydrogenase B chain
    Names
    LDH heart subunit
    lactate dehydrogenase 2, B chain
    NP_001289694.1
    NP_001303251.1
    NP_032518.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001302765.1 → NP_001289694.1  L-lactate dehydrogenase B chain isoform 2

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) lacks an exon in the 3' coding region, which results in a frameshift and a different 3' UTR compared to variant 1. The encoded isoform (2) is shorter with a distinct C-terminus compared to isoform Ldhb.
      Source sequence(s)
      AK019391, BC046755, CX208147
      UniProtKB/Swiss-Prot
      P16125
      Conserved Domains (1) summary
      cd05293
      Location:20 → 246
      LDH_1; A subgroup of L-lactate dehydrogenases

    2. NM_001316322.1 → NP_001303251.1  L-lactate dehydrogenase B chain isoform Ldhbx

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (Ldhb) results from translation termination at the upstream UGA stop codon, while the longer isoform (Ldhbx) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the longer, C-terminally extended isoform (Ldhbx), which is localized in the peroxisomes. As the UGA stop codon has been reported to specify several alternative amino acids (tryptophan, cysteine, arginine and serine), its location in the longer isoform is denoted by an 'X'.
      Source sequence(s)
      AK019391, BC046755
      UniProtKB/TrEMBL
      A0A6I8MX27
      Related
      ENSMUSP00000159378.2, ENSMUST00000239395.2
      Conserved Domains (2) summary
      cd05293
      Location:20 → 330
      LDH_1; A subgroup of L-lactate dehydrogenases
      TIGR01771
      Location:26 → 326
      L-LDH-NAD; L-lactate dehydrogenase

    3. NM_008492.3 → NP_032518.1  L-lactate dehydrogenase B chain isoform Ldhb

      See identical proteins and their annotated locations for NP_032518.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (Ldhb) results from translation termination at the upstream UGA stop codon, while the longer isoform (Ldhbx) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the shorter isoform (Ldhb), which is localized in the cytosol.
      Source sequence(s)
      AK019391, BC046755
      Consensus CDS
      CCDS20684.1
      UniProtKB/Swiss-Prot
      P16125, Q545Y4
      Related
      ENSMUSP00000032373.6, ENSMUST00000032373.12
      Conserved Domains (2) summary
      cd05293
      Location:20 → 330
      LDH_1; A subgroup of L-lactate dehydrogenases
      TIGR01771
      Location:26 → 326
      L-LDH-NAD; L-lactate dehydrogenase

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000072.7 Reference GRCm39 C57BL/6J

      Range
      142435975..142453683 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P16125.2 GenPept UniProtKB/Swiss-Prot:P16125

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