SIW14 putative tyrosine protein phosphatase SIW14 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: SIW14
Gene description: putative tyrosine protein phosphatase SIW14
Primary source: FungiDB:YNL032W
Locus tag: YNL032W
See related: SGD:S000004977; AllianceGenome:SGD:S000004977; VEuPathDB:YNL032W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: OCA3
Summary: Enables inositol-1,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 5-diphosphatase activity; inositol-5-diphosphate-1,2,3,4,6-pentakisphosphate diphosphatase activity; and inositol-5-diphosphate-1,3,4,6-tetrakisphosphate diphosphatase activity. Involved in actin filament organization; diphosphoinositol polyphosphate metabolic process; and inositol phosphate dephosphorylation. Located in cytosol. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See SIW14 in Genome Data Viewer

Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (574506..575351)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The InsP7 phosphatase Siw14 regulates inositol pyrophosphate levels to control localization of the general stress response transcription factor Msn2.
Title: The InsP(7) phosphatase Siw14 regulates inositol pyrophosphate levels to control localization of the general stress response transcription factor Msn2.
three structural elements that demarcate a 9.2-A-deep substrate-binding pocket each have spatial equivalents in PTPs, but we identify how these are specialized for Siw14 to bind and hydrolyze the intensely negatively charged PP-InsPs
Title: Structural and biochemical characterization of Siw14: A protein-tyrosine phosphatase fold that metabolizes inositol pyrophosphates.
Siw14 is a novel inositol pyrophosphate phosphatase in Saccharomyces cerevisiae.
Title: A Novel Inositol Pyrophosphate Phosphatase in Saccharomyces cerevisiae: Siw14 PROTEIN SELECTIVELY CLEAVES THE β-PHOSPHATE FROM 5-DIPHOSPHOINOSITOL PENTAKISPHOSPHATE (5PP-IP5).
we conclude that Siw14 functions upstream of Pph21 and Pph22 as an inhibitor of the phosphorylation and localization of Gln3, and that Sit4 acts independently of Siw14
Title: The protein phosphatase Siw14 controls caffeine-induced nuclear localization and phosphorylation of Gln3 via the type 2A protein phosphatases Pph21 and Pph22 in Saccharomyces cerevisiae.
Siw14 controls the intracellular localization of Gln3 in combination with Npr1, and one of the causes for the caffeine sensitivity of the Deltasiw14 disruptant was an accumulation of dephosphorylated Gln3 in the nucleus.
Title: Protein phosphatase Siw14 controls intracellular localization of Gln3 in cooperation with Npr1 kinase in Saccharomyces cerevisiae.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables diphosphoinositol-polyphosphate diphosphatase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables inositol diphosphate tetrakisphosphate diphosphatase activity	IBA Inferred from Biological aspect of Ancestor more info
enables inositol-1,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 5-diphosphatase activity	IDA Inferred from Direct Assay more info	PubMed
enables inositol-1,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 5-diphosphatase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables inositol-3,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 5-diphosphatase activity	IEA Inferred from Electronic Annotation more info
enables inositol-5-diphosphate-1,2,3,4,6-pentakisphosphate diphosphatase activity	IDA Inferred from Direct Assay more info	PubMed
enables inositol-5-diphosphate-1,2,3,4,6-pentakisphosphate diphosphatase activity	IEA Inferred from Electronic Annotation more info
enables inositol-5-diphosphate-1,2,3,4,6-pentakisphosphate diphosphatase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables inositol-5-diphosphate-1,3,4,6-tetrakisphosphate diphosphatase activity	IDA Inferred from Direct Assay more info	PubMed
enables inositol-5-diphosphate-1,3,4,6-tetrakisphosphate diphosphatase activity	IEA Inferred from Electronic Annotation more info
enables inositol-5-diphosphate-1,3,4,6-tetrakisphosphate diphosphatase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables phosphatase activity	IBA Inferred from Biological aspect of Ancestor more info
enables phosphatase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in actin filament organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in diphosphoinositol polyphosphate metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	HDA more info	PubMed

General protein information

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Preferred Names: putative tyrosine protein phosphatase SIW14

NP_014366.3

EC 3.6.1.52

Inositol phosphatase involved in inositol pyrophosphate metabolism; hydrolyzes the beta-phosphate from the 5-diphosphate group of PP-InsPs including: 5PP-IP5 (IP7), 1,5PP-IP4 (IP8) and 5-PP-InsP4; functions as an anti-prion (anti-[PSI]) component; member of the atypical dual-specificity subgroup of the protein tyrosine-phosphatase (PTP) superfamily; localizes to the cytoplasm

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001146.8 Reference assembly

Range

574506..575351

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182871.3 → NP_014366.3 TPA: putative tyrosine protein phosphatase SIW14 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014366.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W1E7, P53965

UniProtKB/TrEMBL

A0A8H4F7R3, A6ZS58, B3LPQ4, C7GWP9, C8ZFK9, G2WL39, N1NXM3

Conserved Domains (2) summary

COG2365
Location:1 → 281

Oca4; Protein tyrosine/serine phosphatase [Signal transduction mechanisms]

cd14528
Location:115 → 263

PFA-DSP_Siw14; atypical dual specificity phosphatases similar to yeast Siw14