Cenpa centromere protein A [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Cenpaprovided by MGI
Official Full Name: centromere protein Aprovided by MGI
Primary source: MGI:MGI:88375
See related: Ensembl:ENSMUSG00000029177 AllianceGenome:MGI:88375
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Cenp-A
Summary: Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. This gene encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. Centromere protein A is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. The protein is a replication-independent histone that is a member of the histone H3 family. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Nov 2015]
Expression: Broad expression in CNS E11.5 (RPKM 43.5), liver E14.5 (RPKM 41.9) and 20 other tissues See more
Orthologs: human all
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Genomic context

Location:: 5 B1; 5 16.76 cM

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (30824214..30832181)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (30666877..30674837)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Variation in the CENP-A sequence association landscape across diverse inbred mouse strains.
Title: Variation in the CENP-A sequence association landscape across diverse inbred mouse strains.
Ser68 phosphoregulation is essential for CENP-A deposition, centromere function and viability in mice.
Title: Ser68 phosphoregulation is essential for CENP-A deposition, centromere function and viability in mice.
CENP-A nucleosome retention at centromeres requires a core centromeric nucleosome complex where CENP-C clamps down a stable nucleosome conformation and CENP-N fastens CENP-A to the DNA.
Title: Centromeres are maintained by fastening CENP-A to DNA and directing an arginine anchor-dependent nucleosome transition.
Centromeres with more satellite repeats house more nucleosomes that confer centromere identity, containing the histone H3 variant CENP-A, and bias their segregation to the egg relative to centromeres with fewer repeats.
Title: Expanded Satellite Repeats Amplify a Discrete CENP-A Nucleosome Assembly Site on Chromosomes that Drive in Female Meiosis.
The findings provide evidence that CENP-A is critical for the faithful chromosome segregation during mammalian oocyte meiosis.
Title: CENP-A regulates chromosome segregation during the first meiosis of mouse oocytes.
evolutionarily conserved flexible ends of the CENP-A nucleosomes are essential to ensure the fidelity of the mitotic pathway.
Title: The Flexible Ends of CENP-A Nucleosome Are Required for Mitotic Fidelity.
These data implicate the insulin-FoxM1/PLK1/CENP-A pathway-regulated mitotic cell-cycle progression as an essential component in the beta cell adaptation to delay and/or prevent progression to diabetes.
Title: Insulin Signaling Regulates the FoxM1/PLK1/CENP-A Pathway to Promote Adaptive Pancreatic β Cell Proliferation.
CPCs maintain relatively high levels of CENP-A early in life, which is necessary for sustaining proliferation, inhibiting senescence, and promoting survival following differentiation of CPCs.
Title: CENP-A is essential for cardiac progenitor cell proliferation.
Results report that the level of CENP-A, a protein required for cell division, declines precipitously with age in an islet-specific manner.
Title: CENP-A, a protein required for chromosome segregation in mitosis, declines with age in islet but not exocrine cells.
The centromere-targeting domain of CENP-A is both necessary and sufficient for recruitment to double-strand breaks. CENP-A accumulation at DNA breaks is enhanced by active non-homologous end-joining but does not require DNA-PKcs or Ligase IV.
Title: Double-strand DNA breaks recruit the centromeric histone CENP-A.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2) 1 citation
Targeted (5) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AF012709 (e-PCR)
- UniSTS:159353

NoName (e-PCR)
- UniSTS:235154

PMC15551P1 (e-PCR)
- UniSTS:271341

PMC15551P2 (e-PCR)
- UniSTS:271342

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables protein heterodimerization activity	IEA Inferred from Electronic Annotation more info
enables structural constituent of chromatin	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in CENP-A containing chromatin assembly	IEA Inferred from Electronic Annotation more info
involved_in CENP-A containing chromatin assembly	ISO Inferred from Sequence Orthology more info
involved_in establishment of mitotic spindle orientation	IEA Inferred from Electronic Annotation more info
involved_in establishment of mitotic spindle orientation	ISO Inferred from Sequence Orthology more info
involved_in kinetochore assembly	IEA Inferred from Electronic Annotation more info
involved_in kinetochore assembly	ISO Inferred from Sequence Orthology more info
involved_in mitotic cytokinesis	IEA Inferred from Electronic Annotation more info
involved_in mitotic cytokinesis	ISO Inferred from Sequence Orthology more info
involved_in protein localization to CENP-A containing chromatin	NAS Non-traceable Author Statement more info	PubMed
acts_upstream_of_or_within protein localization to chromosome, centromeric region	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to chromosome, centromeric region	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in CENP-A containing chromatin	IDA Inferred from Direct Assay more info	PubMed
part_of CENP-A containing nucleosome	ISO Inferred from Sequence Orthology more info
located_in chromosome, centromeric region	IDA Inferred from Direct Assay more info	PubMed
located_in chromosome, centromeric region	ISO Inferred from Sequence Orthology more info	PubMed
located_in condensed chromosome, centromeric region	IDA Inferred from Direct Assay more info	PubMed
located_in condensed chromosome, centromeric region	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
part_of nucleosome	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in pericentric heterochromatin	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: histone H3-like centromeric protein A

Names: centromere autoantigen A; centrosomin A

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001302129.1 → NP_001289058.1 histone H3-like centromeric protein A isoform 2

See identical proteins and their annotated locations for NP_001289058.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) contains an alternate exon in the 5' coding region and uses a downstream start codon compared to variant 1. The resulting isoform (2) has a distinct shorter N-terminus, compared to isoform 1. Variants 2, 3 and 4 encode the same isoform (2).

Source sequence(s)

AC105298, AF012709, AK011399, AK041138, BQ748418, BY136144

UniProtKB/Swiss-Prot

O35216

Conserved Domains (1) summary

smart00428
Location:3 → 105

H3; Histone H3
NM_001302130.1 → NP_001289059.1 histone H3-like centromeric protein A isoform 2

See identical proteins and their annotated locations for NP_001289059.1

Status: REVIEWED

Description

Transcript Variant: This variant (3) contains two alternate exons in the 5' coding region and uses a downstream start codon compared to variant 1. The resulting isoform (2) has a distinct shorter N-terminus, compared to isoform 1. Variants 2, 3 and 4 encode the same isoform (2).

Source sequence(s)

AC105298, AF012709, AK011399, AK041138, BQ748418, BY136144

UniProtKB/Swiss-Prot

O35216

Conserved Domains (1) summary

smart00428
Location:3 → 105

H3; Histone H3
NM_001302131.1 → NP_001289060.1 histone H3-like centromeric protein A isoform 2

See identical proteins and their annotated locations for NP_001289060.1

Status: REVIEWED

Description

Transcript Variant: This variant (4) contains two alternate exons in the 5' coding region and uses a downstream start codon compared to variant 1. The resulting isoform (2) has a distinct shorter N-terminus, compared to isoform 1. Variants 2, 3 and 4 encode the same isoform (2).

Source sequence(s)

AC105298, AF012709, AK011399, AK041138, BQ748418, BY136144

UniProtKB/Swiss-Prot

O35216

Conserved Domains (1) summary

smart00428
Location:3 → 105

H3; Histone H3
NM_001302132.1 → NP_001289061.1 histone H3-like centromeric protein A isoform 3

Status: REVIEWED

Description

Transcript Variant: This variant (5) lacks a 3' exon, which results in a frameshift, compared to variant 1. The resulting isoform (3) has a shorter and distinct C-terminus, compared to isoform 1.

Source sequence(s)

AA016357, AC105298, AF012709, AK011399, BQ748418, BY136144

UniProtKB/TrEMBL

A0A0G2JGI2

Related

ENSMUSP00000143575.2, ENSMUST00000199320.5

Conserved Domains (1) summary

cl23735
Location:28 → 90

H4; Histone H4, one of the four histones, along with H2A, H2B and H3, which forms the eukaryotic nucleosome core; along with H3, it plays a central role in nucleosome formation; histones bind to DNA and wrap the genetic material into "beads on a string" in ...
NM_001421447.1 → NP_001408376.1 histone H3-like centromeric protein A isoform 2

Status: REVIEWED

Source sequence(s)

AC105298
NM_001421448.1 → NP_001408377.1 histone H3-like centromeric protein A isoform 4

Status: REVIEWED

Source sequence(s)

AC105298
NM_001421449.1 → NP_001408378.1 histone H3-like centromeric protein A isoform 5

Status: REVIEWED

Source sequence(s)

AC105298
NM_007681.3 → NP_031707.1 histone H3-like centromeric protein A isoform 1

See identical proteins and their annotated locations for NP_031707.1

Status: REVIEWED

Description

Transcript Variant: This variant (1) encodes the longest isoform (1).

Source sequence(s)

AC105298, AF012709, AK011399, BQ748418, BY136144

Consensus CDS

CCDS19162.1

UniProtKB/Swiss-Prot

O35216, Q545C9

Related

ENSMUSP00000122831.2, ENSMUST00000144742.6

Conserved Domains (2) summary

smart00428
Location:28 → 131

H3; Histone H3

pfam00125
Location:1 → 127

Histone; Core histone H2A/H2B/H3/H4

RNA

NR_126074.1 RNA Sequence

Status: REVIEWED

Description

Transcript Variant: This variant (6) uses an alternate splice site in the 3' region compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AC105298, AF012709, AK011399, BQ748418, BY136144
NR_185302.1 RNA Sequence

Status: REVIEWED

Source sequence(s)

AC105298
NR_185303.1 RNA Sequence

Status: REVIEWED

Source sequence(s)

AC105298
NR_185304.1 RNA Sequence

Status: REVIEWED

Source sequence(s)

AC105298

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000071.7 Reference GRCm39 C57BL/6J

Range

30824214..30832181

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036164762.1 → XP_036020655.1 histone H3-like centromeric protein A isoform X2

Conserved Domains (1) summary

smart00428
Location:3 → 105

H3; Histone H3