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    Med16 mediator complex subunit 16 [ Mus musculus (house mouse) ]

    Gene ID: 216154, updated on 2-Nov-2024

    Summary

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    Official Symbol
    Med16provided by MGI
    Official Full Name
    mediator complex subunit 16provided by MGI
    Primary source
    MGI:MGI:2158394
    See related
    Ensembl:ENSMUSG00000013833 AllianceGenome:MGI:2158394
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    95kDa; DRIP92; Thrap5; Trap95; A630083L04
    Summary
    Predicted to enable nuclear thyroid hormone receptor binding activity and transcription coactivator activity. Predicted to be involved in positive regulation of transcription initiation by RNA polymerase II. Predicted to act upstream of or within transcription by RNA polymerase II. Part of mediator complex. Is expressed in genitourinary system; limb; and tongue. Orthologous to human MED16 (mediator complex subunit 16). [provided by Alliance of Genome Resources, Nov 2024]
    Expression
    Ubiquitous expression in testis adult (RPKM 63.2), ovary adult (RPKM 52.5) and 28 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Med16 in Genome Data Viewer
    Location:
    10 C1; 10 39.72 cM
    Exon count:
    16
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 10 NC_000076.7 (79730543..79744757, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 10 NC_000076.6 (79894706..79908938, complement)

    Chromosome 10 - NC_000076.7Genomic Context describing neighboring genes Neighboring gene 40S ribosomal protein S21 pseudogene Neighboring gene complement factor D Neighboring gene STARR-positive B cell enhancer ABC_E2946 Neighboring gene STARR-positive B cell enhancer ABC_E252 Neighboring gene predicted gene, 24019 Neighboring gene R3H domain containing 4

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. The Mediator Subunit MED16 Transduces NRF2-Activating Signals into Antioxidant Gene Expression. Sekine H, et al. Mol Cell Biol, 2016 Feb 1. PMID 26572828, Free PMC Article
    2. The TRAP100 component of the TRAP/Mediator complex is essential in broad transcriptional events and development. Ito M, et al. EMBO J, 2002 Jul 1. PMID 12093747, Free PMC Article
    3. Mediator complex proximal Tail subunit MED30 is critical for Mediator core stability and cardiomyocyte transcriptional network. Tan C, et al. PLoS Genet, 2021 Sep. PMID 34506481, Free PMC Article
    4. Temporal regulation of the Mediator complex during muscle proliferation, differentiation, regeneration, aging, and disease. Kolonay DW, et al. Front Cell Dev Biol, 2024. PMID 38690566, Free PMC Article
    5. Structural and functional organization of TRAP220, the TRAP/mediator subunit that is targeted by nuclear receptors. Malik S, et al. Mol Cell Biol, 2004 Sep. PMID 15340084, Free PMC Article

    See all (29) citations in PubMed

    GeneRIFs: Gene References Into Functions

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (2) 
    • Targeted (2) 

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables nuclear thyroid hormone receptor binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables nuclear thyroid hormone receptor binding ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    enables transcription coactivator activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables transcription coactivator activity ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    Process Evidence Code Pubs
    involved_in RNA polymerase II preinitiation complex assembly NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in positive regulation of DNA-templated transcription IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in positive regulation of DNA-templated transcription ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in positive regulation of DNA-templated transcription ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    involved_in positive regulation of transcription elongation by RNA polymerase II NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in positive regulation of transcription initiation by RNA polymerase II ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in positive regulation of transcription initiation by RNA polymerase II ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    involved_in positive regulation of transcription initiation by RNA polymerase II NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    acts_upstream_of_or_within transcription by RNA polymerase II IC
    Inferred by Curator
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of core mediator complex ISO
    Inferred from Sequence Orthology
    more info
    		  
    part_of mediator complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of mediator complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of mediator complex ISO
    Inferred from Sequence Orthology
    more info
    		  
    part_of mediator complex ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    located_in nucleoplasm TAS
    Traceable Author Statement
    more info
    		  
    located_in nucleus ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    mediator of RNA polymerase II transcription subunit 16
    Names
    thyroid hormone receptor associated protein 5
    thyroid hormone receptor-associated protein complex 95 kDa component
    thyroid hormone receptor-associated protein, 95-kD subunit
    vitamin D3 receptor-interacting protein complex 92 kDa component

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001163276.2 → NP_001156748.2  mediator of RNA polymerase II transcription subunit 16 isoform 1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) uses a different splice site in the 5' coding region, compared to variant 1. The resulting protein (isoform 2) is shorter by 1 aa when it is compared to isoform 1.
      Source sequence(s)
      AC151846
      Consensus CDS
      CCDS48623.1
      UniProtKB/Swiss-Prot
      E9QM81, Q6PGF3
      Related
      ENSMUSP00000101017.4, ENSMUST00000105378.9

    2. NM_001359629.2 → NP_001346558.2  mediator of RNA polymerase II transcription subunit 16 isoform 3

      Status: VALIDATED

      Description
      Transcript Variant: This variant (3) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. The resulting protein (isoform 3), has a shorter and distinct C-terminus, compared to isoform 1.
      Source sequence(s)
      AC151846

    3. NM_001403459.1 → NP_001390388.1  mediator of RNA polymerase II transcription subunit 16 isoform 1

      Status: VALIDATED

      Source sequence(s)
      AC151846
      UniProtKB/Swiss-Prot
      E9QM81, Q6PGF3

    4. NM_198107.3 → NP_932775.3  mediator of RNA polymerase II transcription subunit 16 isoform 1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) encodes the longest protein (isoform 1).
      Source sequence(s)
      AC151846
      Consensus CDS
      CCDS23996.2
      UniProtKB/Swiss-Prot
      E9QM81, Q6PGF3
      Related
      ENSMUSP00000129375.3, ENSMUST00000165684.8

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000076.7 Reference GRCm39 C57BL/6J

      Range
      79730543..79744757 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_036155735.1 → XP_036011628.1  mediator of RNA polymerase II transcription subunit 16 isoform X1

      UniProtKB/TrEMBL
      E9QP84
      Conserved Domains (3) summary
      smart00320
      Location:59 → 98
      WD40; WD40 repeats
      sd00039
      Location:26 → 68
      7WD40; WD40 repeat [structural motif]
      pfam11635
      Location:108 → 822
      Med16; Mediator complex subunit 16
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q6PGF3.2 GenPept UniProtKB/Swiss-Prot:Q6PGF3

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

    Other Literature Sources
    Chemical Information
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