Nos1 nitric oxide synthase 1, neuronal [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Nos1provided by MGI
Official Full Name: nitric oxide synthase 1, neuronalprovided by MGI
Primary source: MGI:MGI:97360
See related: Ensembl:ENSMUSG00000029361 AllianceGenome:MGI:97360
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: NO; NOS; bNOS; nNOS; N-NOS; NOS-I; Nos-1; NC-NOS; 2310005C01Rik
Summary: Enables nitric-oxide synthase activity. Involved in several processes, including nitric oxide biosynthetic process; positive regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway; and positive regulation of the force of heart contraction. Acts upstream of or within several processes, including peptidyl-cysteine S-nitrosylation; positive regulation of transcription by RNA polymerase II; and regulation of metal ion transport. Located in several cellular components, including T-tubule; Z disc; and sarcoplasmic reticulum membrane. Is active in calyx of Held. Colocalizes with caveola. Is expressed in several structures, including alimentary system; genitourinary system; integumental system; nervous system; and sensory organ. Used to study achalasia and hypertrophic pyloric stenosis. Human ortholog(s) of this gene implicated in Alzheimer's disease; Parkinson's disease; asthma; and cystic fibrosis. Orthologous to human NOS1 (nitric oxide synthase 1). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in cerebellum adult (RPKM 7.6), frontal lobe adult (RPKM 4.0) and 12 other tissues See more
Orthologs: human all
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Genomic context

Location:: 5 F; 5 57.29 cM

Exon count:: 28

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (118004904..118096905)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (117866839..117958840)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Restoration of nNOS Expression Rescues Autistic-Like Phenotypes Through Normalization of AMPA Receptor-Mediated Neurotransmission.
Title: Restoration of nNOS Expression Rescues Autistic-Like Phenotypes Through Normalization of AMPA Receptor-Mediated Neurotransmission.
A role for nNOS in mediating stress and female sexual behavior in mice.
Title: A role for nNOS in mediating stress and female sexual behavior in mice.
Expression of neuronal NO synthase alpha- and beta-isoforms in skeletal muscle of mice.
Title: Expression of neuronal NO synthase α- and β-isoforms in skeletal muscle of mice.
nNOS in Erbb4-positive neurons regulates GABAergic transmission in mouse hippocampus.
Title: nNOS in Erbb4-positive neurons regulates GABAergic transmission in mouse hippocampus.
The pivotal role of neuronal nitric oxide synthase in the release of 6-nitrodopamine from mouse isolated vas deferens.
Title: The pivotal role of neuronal nitric oxide synthase in the release of 6-nitrodopamine from mouse isolated vas deferens.
Hypoxia-Inducible Factor 1alpha Stabilization Restores Epigenetic Control of Nitric Oxide Synthase 1 Expression and Reverses Gastroparesis in Female Diabetic Mice.
Title: Hypoxia-Inducible Factor 1α Stabilization Restores Epigenetic Control of Nitric Oxide Synthase 1 Expression and Reverses Gastroparesis in Female Diabetic Mice.
Calcium transients in nNOS neurons underlie distinct phases of the neurovascular response to barrel cortex activation in awake mice.
Title: Calcium transients in nNOS neurons underlie distinct phases of the neurovascular response to barrel cortex activation in awake mice.
Neuronal Nitric Oxide Synthase Critically Regulates the Endocannabinoid Pathway in the Murine Cerebellum During Development.
Title: Neuronal Nitric Oxide Synthase Critically Regulates the Endocannabinoid Pathway in the Murine Cerebellum During Development.
nNOS Increases Fiber Type-Specific Angiogenesis in Skeletal Muscle of Mice in Response to Endurance Exercise.
Title: nNOS Increases Fiber Type-Specific Angiogenesis in Skeletal Muscle of Mice in Response to Endurance Exercise.
Nos1[+] and Nos1[-] excitatory neurons in the BLA regulate anxiety- and depression-related behaviors oppositely.
Title: Nos1(+) and Nos1(-) excitatory neurons in the BLA regulate anxiety- and depression-related behaviors oppositely.

Submit: New GeneRIF Correction See all GeneRIFs (339)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (6)
Targeted (6) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

PMC311332P1 (e-PCR)
- UniSTS:272900

D5Mit188 (e-PCR), detects polymorphism
- UniSTS:130319

D5Mit210 (e-PCR), detects polymorphism
- UniSTS:130342

GDB:437697 (e-PCR)
- UniSTS:157252

Nos1 (e-PCR), detects polymorphism
- UniSTS:525458

D5Mit319 (e-PCR), detects polymorphism
- UniSTS:130461

Nos1 (e-PCR), detects polymorphism
- UniSTS:144038

Nos1 (e-PCR), detects polymorphism
- UniSTS:507095

PMC59521P1 (e-PCR)
- UniSTS:273374

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATPase binding	ISO Inferred from Sequence Orthology more info
enables FMN binding	IBA Inferred from Biological aspect of Ancestor more info
enables FMN binding	IEA Inferred from Electronic Annotation more info
enables FMN binding	ISO Inferred from Sequence Orthology more info
enables NADP binding	IEA Inferred from Electronic Annotation more info
enables NADP binding	ISO Inferred from Sequence Orthology more info
enables NADPH binding	ISO Inferred from Sequence Orthology more info
enables cadmium ion binding	ISO Inferred from Sequence Orthology more info
enables calcium-dependent protein binding	ISO Inferred from Sequence Orthology more info
enables calmodulin binding	IEA Inferred from Electronic Annotation more info
enables calmodulin binding	ISO Inferred from Sequence Orthology more info
enables enzyme binding	ISO Inferred from Sequence Orthology more info
enables flavin adenine dinucleotide binding	IBA Inferred from Biological aspect of Ancestor more info
enables flavin adenine dinucleotide binding	IEA Inferred from Electronic Annotation more info
enables flavin adenine dinucleotide binding	ISO Inferred from Sequence Orthology more info
enables heme binding	IEA Inferred from Electronic Annotation more info
enables heme binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nitric-oxide synthase activity	IBA Inferred from Biological aspect of Ancestor more info
enables nitric-oxide synthase activity	IDA Inferred from Direct Assay more info	PubMed
enables nitric-oxide synthase activity	ISO Inferred from Sequence Orthology more info
enables phosphoprotein binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables scaffold protein binding	ISO Inferred from Sequence Orthology more info
enables sodium channel regulator activity	ISO Inferred from Sequence Orthology more info
enables transmembrane transporter binding	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in arginine catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in arginine catabolic process	ISO Inferred from Sequence Orthology more info
involved_in behavioral response to cocaine	ISO Inferred from Sequence Orthology more info
acts_upstream_of calcium ion transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to epinephrine stimulus	ISO Inferred from Sequence Orthology more info
involved_in cellular response to growth factor stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to mechanical stimulus	ISO Inferred from Sequence Orthology more info
acts_upstream_of establishment of localization in cell	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of protein localization	ISO Inferred from Sequence Orthology more info
involved_in multicellular organismal response to stress	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of blood pressure	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of blood pressure	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of calcium ion transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of cell population proliferation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cytosolic calcium ion concentration	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of hepatic stellate cell contraction	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of hydrolase activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of insulin secretion	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of iron ion transmembrane transport	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of neuron apoptotic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of potassium ion transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of serotonin uptake	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of vasoconstriction	ISO Inferred from Sequence Orthology more info
involved_in nitric oxide biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in nitric oxide biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in nitric oxide biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in nitric oxide mediated signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in nitric oxide-cGMP-mediated signaling	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within peptidyl-cysteine S-nitrosylation	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of positive regulation of adenylate cyclase-activating adrenergic receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of long-term synaptic potentiation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of peptidyl-serine phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of sodium ion transmembrane transport	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of the force of heart contraction	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of potassium ion transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of neurogenesis	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of postsynaptic membrane potential	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of postsynaptic membrane potential	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of sodium ion transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to estrogen	ISO Inferred from Sequence Orthology more info
involved_in response to heat	ISO Inferred from Sequence Orthology more info
involved_in response to hormone	IBA Inferred from Biological aspect of Ancestor more info
involved_in response to lipopolysaccharide	IBA Inferred from Biological aspect of Ancestor more info
involved_in response to peptide hormone	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within striated muscle contraction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in synaptic signaling by nitric oxide	IDA Inferred from Direct Assay more info	PubMed
involved_in synaptic signaling by nitric oxide	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in vasodilation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within xenobiotic catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in T-tubule	IDA Inferred from Direct Assay more info	PubMed
located_in Z disc	IDA Inferred from Direct Assay more info	PubMed
located_in azurophil granule	ISO Inferred from Sequence Orthology more info
is_active_in calyx of Held	IDA Inferred from Direct Assay more info	PubMed
is_active_in calyx of Held	IMP Inferred from Mutant Phenotype more info	PubMed
colocalizes_with caveola	IDA Inferred from Direct Assay more info	PubMed
located_in cell periphery	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in dendrite	ISO Inferred from Sequence Orthology more info
located_in dendritic spine	IEA Inferred from Electronic Annotation more info
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
located_in membrane raft	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial outer membrane	ISO Inferred from Sequence Orthology more info
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	ISO Inferred from Sequence Orthology more info	PubMed
located_in nuclear membrane	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info
located_in photoreceptor inner segment	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in postsynaptic density	IBA Inferred from Biological aspect of Ancestor more info
located_in postsynaptic density	ISO Inferred from Sequence Orthology more info
is_active_in postsynaptic density, intracellular component	ISO Inferred from Sequence Orthology more info
is_active_in postsynaptic specialization, intracellular component	ISO Inferred from Sequence Orthology more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info
located_in sarcolemma	IDA Inferred from Direct Assay more info	PubMed
located_in sarcolemma	ISO Inferred from Sequence Orthology more info
located_in sarcoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in sarcoplasmic reticulum membrane	IDA Inferred from Direct Assay more info	PubMed
located_in secretory granule	ISO Inferred from Sequence Orthology more info
located_in synapse	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: nitric oxide synthase 1

Names: NOS type I; constitutive NOS; neuronal NOS; neuronal nitric oxide synthase; nitric oxide synthase, brain; peptidyl-cysteine S-nitrosylase NOS1

NP_032738.1

EC 1.14.13.39

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_008712.3 → NP_032738.1 nitric oxide synthase 1

See identical proteins and their annotated locations for NP_032738.1

Status: VALIDATED

Source sequence(s)

AC114617, AC114993

Consensus CDS

CCDS19606.1

UniProtKB/Swiss-Prot

Q3UR10, Q64208, Q9Z0J4

UniProtKB/TrEMBL

S4R255

Related

ENSMUSP00000127432.2, ENSMUST00000171055.2

Conserved Domains (5) summary

cd06202
Location:996 → 1399

Nitric_oxide_synthase; The ferredoxin-reductase (FNR) like C-terminal domain of the nitric oxide synthase (NOS) fuses with a heme-containing N-terminal oxidase domain. The reductase portion is similar in structure to NADPH dependent cytochrome-450 reductase (CYPOR), having an ...

COG0369
Location:754 → 1394

CysJ; Sulfite reductase, alpha subunit (flavoprotein) [Inorganic ion transport and metabolism]

cd00795
Location:300 → 711

NOS_oxygenase_euk; Nitric oxide synthase (NOS) eukaryotic oxygenase domain. NOS produces nitric oxide (NO) by catalyzing a five-electron heme-based oxidation of a guanidine nitrogen of L-arginine to L-citrulline via two successive monooxygenation reactions producing N ...

cd00992
Location:15 → 98

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

pfam00258
Location:757 → 930

Flavodoxin_1; Flavodoxin