MSG5 tyrosine/serine/threonine protein phosphatase MSG5 [Saccharomyces cerevisiae S288C] - Gene

Summary

Go to the top of the page Help

Official Symbol: MSG5
Official Full Name: tyrosine/serine/threonine protein phosphatase MSG5
Primary source: SGD:S000004998
Locus tag: YNL053W
See related: AllianceGenome:SGD:S000004998; FungiDB:YNL053W; VEuPathDB:YNL053W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables protein tyrosine/serine/threonine phosphatase activity. Involved in several processes, including negative regulation of cell integrity MAPK cascade; regulation of fungal-type cell wall organization; and response to pheromone triggering conjugation with cellular fusion. Located in cytoplasm and nucleus. Human ortholog(s) of this gene implicated in breast cancer. Orthologous to several human genes including DUSP1 (dual specificity phosphatase 1); DUSP10 (dual specificity phosphatase 10); and DUSP16 (dual specificity phosphatase 16). [provided by Alliance of Genome Resources, Nov 2024]
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help

See MSG5 in Genome Data Viewer

Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (529942..531411)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

PTP2 and MSG5 are key regulatory PPases that prevent over-activation of the calcium-induced signaling cascade under the parallel control of the SLT2 and calcineurin pathways.
Title: Functionally redundant protein phosphatase genes PTP2 and MSG5 co-regulate the calcium signaling pathway in Saccharomyces cerevisiae upon exposure to high extracellular calcium concentration.
analysis of distinct docking mechanisms mediate interactions between the Msg5 phosphatase and mating or cell integrity mitogen-activated protein kinases (MAPKs) in Saccharomyces cerevisiae
Title: Distinct docking mechanisms mediate interactions between the Msg5 phosphatase and mating or cell integrity mitogen-activated protein kinases (MAPKs) in Saccharomyces cerevisiae.
Data discovered that six non-essential protein kinase (PKase) disruptions (Deltabck1, Deltamkk1, Deltaslt2/Deltampk1, Deltamck1, Deltassk2 and Deltayak1) suppressed the Cas-phenotype of the Deltaptp2 Deltamsg5 double disruptant.
Title: Identification of protein kinase disruptions as suppressors of the calcium sensitivity of S. cerevisiae Deltaptp2 Deltamsg5 protein phosphatase double disruptant.
These findings suggest that both Ptp2p and Msg5p are involved in the G1 to S transition and vacuole morphogenesis possibly through their regulation of Slt2 pathway.
Title: Yeast protein phosphatases Ptp2p and Msg5p are involved in G1-S transition, CLN2 transcription, and vacuole morphogenesis.
Both Fus3- and Slt2- but not Kss1-mediated gene expression is induced in cells lacking Msg5.
Title: Different modulation of the outputs of yeast MAPK-mediated pathways by distinct stimuli and isoforms of the dual-specificity phosphatase Msg5.

Submit: New GeneRIF Correction

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables MAP kinase tyrosine phosphatase activity	IBA Inferred from Biological aspect of Ancestor more info
enables MAP kinase tyrosine/serine/threonine phosphatase activity	IBA Inferred from Biological aspect of Ancestor more info
enables phosphoprotein phosphatase activity	IEA Inferred from Electronic Annotation more info
enables protein tyrosine phosphatase activity	IEA Inferred from Electronic Annotation more info
enables protein tyrosine/serine/threonine phosphatase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein tyrosine/threonine phosphatase activity	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in fungal-type cell wall organization or biogenesis	IDA Inferred from Direct Assay more info	PubMed
involved_in fungal-type cell wall organization or biogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in fungal-type cell wall organization or biogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of MAPK cascade	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of cell integrity MAPK cascade	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of cell integrity MAPK cascade	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of cell integrity MAPK cascade	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pheromone response MAPK cascade	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein dephosphorylation	IEA Inferred from Electronic Annotation more info
involved_in regulation of fungal-type cell wall organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in signal transduction	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

Go to the top of the page Help

Preferred Names: tyrosine/serine/threonine protein phosphatase MSG5

NP_014345.3

EC 3.1.3.48

Dual-specificity protein phosphatase; maintains low levels of both basal and induced cell integrity pathway signaling by dephosphorylation of the Slt2p MAPK; reciprocally regulated by Slt2p through phosphorylation; minor role with Ptp2p in the adaptive response to pheromone through the dephosphorylation of the Fus3p MAPK with major contribution by Ptp3p; inhibits the nuclear accumulation of Fus3p; two isoforms exist as the result of alternative translation initiation starts

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001146.8 Reference assembly

Range

529942..531411

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182892.3 → NP_014345.3 TPA: tyrosine/serine/threonine protein phosphatase MSG5 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014345.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W1C6, P38590

UniProtKB/TrEMBL

A6ZS25, B3LNR4, B5VQY9, C7GLU8, C8ZGH5, N1NXK7

Conserved Domains (1) summary

cd14521
Location:230 → 373

DSP_fungal_SDP1-like; dual specificity phosphatase domain of fungal dual specificity protein phosphatase SDP1, MSG5, and similar proteins