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    fis DNA-binding transcriptional dual regulator Fis [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947697, updated on 19-Sep-2024

    Summary

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    Official Symbol
    fis
    Official Full Name
    DNA-binding transcriptional dual regulator Fis
    Primary source
    ECOCYC:EG10317
    Locus tag
    b3261
    See related
    ASAP:ABE-0010703
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3248; nbp
    Summary
    fis transcription is supercoiling-dependent. [More information is available at EcoGene: EG10317]. Fis, "factor for inversion stimulation", is a small DNA-binding and bending protein whose main role appears to be the organization and maintenance of nucleoid structure through direct DNA binding and by modulating gyrase and topoisomerase I production , as well as regulation of other proteins that modulate the nucleoid structure, such as CRP, HNS, and HU. [More information is available at EcoCyc: EG10317].
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    Genomic context

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    Sequence:
    NC_000913.3 (3411271..3411567)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene ribosomal protein L11 methyltransferase Neighboring gene tRNA-dihydrouridine synthase B Neighboring gene DNA adenine methyltransferase Neighboring gene DUF2556 domain-containing protein YhdU

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Influence of Nucleoid-Associated Proteins on DNA Supercoiling. Dahlke K, et al. J Phys Chem B, 2019 Dec 5. PMID 31710235
    2. Fis protein forms DNA topological barriers to confine transcription-coupled DNA supercoiling in Escherichia coli. Dages S, et al. FEBS Lett, 2020 Mar. PMID 31639222, Free PMC Article
    3. Facilitated Dissociation of a Nucleoid Protein from the Bacterial Chromosome. Hadizadeh N, et al. J Bacteriol, 2016 Jun 15. PMID 27044624, Free PMC Article
    4. The Fis protein has a stimulating role in initiation of replication in Escherichia coli in vivo. Flåtten I, et al. PLoS One, 2013. PMID 24358293, Free PMC Article
    5. Silencing of toxic gene expression by Fis. Karambelkar S, et al. Nucleic Acids Res, 2012 May. PMID 22287621, Free PMC Article

    See all (163) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Negative feedback for DARS2-Fis complex by ATP-DnaA supports the cell cycle-coordinated regulation for chromosome replication.
      Title: Negative feedback for DARS2-Fis complex by ATP-DnaA supports the cell cycle-coordinated regulation for chromosome replication.
    2. Fis protein forms DNA topological barriers to confine transcription-coupled DNA supercoiling in Escherichia coli.
      Title: Fis protein forms DNA topological barriers to confine transcription-coupled DNA supercoiling in Escherichia coli.
    3. Influence of Nucleoid-Associated Proteins on DNA Supercoiling.
      Title: Influence of Nucleoid-Associated Proteins on DNA Supercoiling.
    4. Comparisons with structures of DNA complexes containing only Fis or Xis, together with mutant protein and DNA binding studies, support a mechanism for cooperative protein binding solely by DNA allostery.
      Title: Cooperative DNA binding by proteins through DNA shape complementarity.
    5. The prolific transcription factor and chromosome packaging protein Fis displays a strong dependence of its off-rate from the bacterial chromosome on Fis concentration.
      Title: Facilitated Dissociation of a Nucleoid Protein from the Bacterial Chromosome.
    6. Simultaneous binding of IHF and Fis to specific sites within the DARS2 chromosomal site promoted regeneration of ATP-DnaA.
      Title: Timely binding of IHF and Fis to DARS2 regulates ATP-DnaA production and replication initiation.
    7. Authors found that, although oligomers were assembled in the absence of any individual high-affinity DnaA binding site, loss of DnaA binding at peripheral sites eliminated Fis repression, and made binding of both Fis and IHF essential.
      Title: Building the bacterial orisome: high-affinity DnaA recognition plays a role in setting the conformation of oriC DNA.
    8. A positive role for the Fis protein in the initiation of replication.
      Title: The Fis protein has a stimulating role in initiation of replication in Escherichia coli in vivo.
    9. our work identifies a novel role for Fis and incorporates CbpA into the growing network of factors that mediate bacterial chromosome structure.
      Title: E. coli Fis protein insulates the cbpA gene from uncontrolled transcription.
    10. Fis mediates its repressive effect by denying access to RNA polymerase at mom promoter.
      Title: Silencing of toxic gene expression by Fis.
    Submit: New GeneRIF Correction See all GeneRIFs (33)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA-binding transcription activator activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables DNA-binding transcription factor activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA-binding transcription repressor activity IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables core promoter sequence-specific DNA binding IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein homodimerization activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein homodimerization activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables sequence-specific DNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables sequence-specific DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables sequence-specific DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables sequence-specific DNA binding, bending IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables transcription cis-regulatory region binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA-templated transcription IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within chromosome organization IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in negative regulation of DNA-templated transcription IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in positive regulation of DNA recombination IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of DNA-templated transcription IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in provirus excision IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within regulation of DNA-templated transcription IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in regulation of DNA-templated transcription IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within response to radiation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of invertasome IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    located_in nucleoid IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of nucleosome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of protein-DNA complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    DNA-binding transcriptional dual regulator Fis

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3411271..3411567
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417727.1 DNA-binding transcriptional dual regulator Fis [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417727.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A376FVT1
      Conserved Domains (1) summary
      PRK00430
      Location:4 → 98
      fis; DNA-binding transcriptional regulator Fis
    Nucleotide Protein
    Heading Accession and Version

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