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    rlmL fused 23S rRNA m(2)G2445 methyltransferase and 23S rRNA m(7)G2069 methyltransferase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945564, updated on 19-Sep-2024

    Summary

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    Official Symbol
    rlmL
    Official Full Name
    fused 23S rRNA m(2)G2445 methyltransferase and 23S rRNA m(7)G2069 methyltransferase
    Primary source
    ECOCYC:G6488
    Locus tag
    b0948
    See related
    ASAP:ABE-0003211
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0939; rlmK; rlmKL; ycbY
    Summary
    rlmL is a non-essential gene co-transcribed with downstream uup gene. [More information is available at EcoGene: EG13717]. RlmL is the methyltransferase responsible for methylation of 23S rRNA at the N2 position of the G2445 nucleotide. [More information is available at EcoCyc: G6488].
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    Genomic context

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    Sequence:
    NC_000913.3 (1007844..1009952)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene dihydroorotate dehydrogenase, type 2 Neighboring gene cell division protein ZapC Neighboring gene 6-N-hydroxylaminopurine resistance protein Neighboring gene ATP-binding protein Uup Neighboring gene intermembrane transport protein PqiA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Structure of the bifunctional methyltransferase YcbY (RlmKL) that adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA. Wang KT, et al. Nucleic Acids Res, 2012 Jun. PMID 22362734, Free PMC Article
    2. Base methylations in the double-stranded RNA by a fused methyltransferase bearing unwinding activity. Kimura S, et al. Nucleic Acids Res, 2012 May. PMID 22210896, Free PMC Article
    3. Purification, crystallization and preliminary X-ray crystallographic analysis of 23S RNA m(2)G2445 methyltransferase RlmL from Escherichia coli. Wang KT, et al. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2010 Nov 1. PMID 21045301, Free PMC Article
    4. An indigenous posttranscriptional modification in the ribosomal peptidyl transferase center confers resistance to an array of protein synthesis inhibitors. Toh SM, et al. J Mol Biol, 2008 Jul 18. PMID 18554609, Free PMC Article
    5. Characterization of the uup locus and its role in transposon excisions and tandem repeat deletions in Escherichia coli. Reddy M, et al. J Bacteriol, 2000 Apr. PMID 10715006, Free PMC Article

    See all (16) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. E. coli YcbY is a bifunctional rRNA methyltransferase catalyzing the m2G2445 and m7G2069 modifications located on opposite sides of helix 74.
      Title: Structure of the bifunctional methyltransferase YcbY (RlmKL) that adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA.
    2. RlmKL is a novel type of fused methyltransferase bearing dual active sites responsible for different methyl modifications at distinct positions.
      Title: Base methylations in the double-stranded RNA by a fused methyltransferase bearing unwinding activity.
    3. final crystals of RlmL diffracted to 2.2 A resolution
      Title: Purification, crystallization and preliminary X-ray crystallographic analysis of 23S RNA m(2)G2445 methyltransferase RlmL from Escherichia coli.
    4. Results describe the identification of a gene, ycbY, as encoding an enzyme responsible for methylation of 23 S rRNA G2445.
      Title: Identification of Escherichia coli m2G methyltransferases: I. the ycbY gene encodes a methyltransferase specific for G2445 of the 23 S rRNA.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 23S rRNA (guanine(2445)-N(2))-methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables rRNA (guanine-N2-)-methyltransferase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables rRNA (guanine-N2-)-methyltransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables rRNA (guanine-N2-)-methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables rRNA (guanine-N7-)-methyltransferase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables rRNA (guanine-N7-)-methyltransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables rRNA (guanine-N7-)-methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in RNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in methylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in rRNA (guanine-N7)-methylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within rRNA base methylation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in rRNA methylation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in rRNA methylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in rRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IC
    Inferred by Curator
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    fused 23S rRNA m(2)G2445 methyltransferase and 23S rRNA m(7)G2069 methyltransferase
    NP_415468.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1007844..1009952
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415468.1 fused 23S rRNA m(2)G2445 methyltransferase and 23S rRNA m(7)G2069 methyltransferase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415468.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8S7QTG2
      Conserved Domains (1) summary
      PRK11783
      Location:1 → 702
      rlmL; bifunctional 23S rRNA (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA (guanine(2445)-N(2))-methyltransferase RlmL
    Nucleotide Protein
    Heading Accession and Version

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