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    Zp2 zona pellucida glycoprotein 2 [ Mus musculus (house mouse) ]

    Gene ID: 22787, updated on 2-Nov-2024

    Summary

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    Official Symbol
    Zp2provided by MGI
    Official Full Name
    zona pellucida glycoprotein 2provided by MGI
    Primary source
    MGI:MGI:99214
    See related
    Ensembl:ENSMUSG00000030911 AllianceGenome:MGI:99214
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    Zp-2
    Summary
    This gene encodes a member of the zona pellucida family of glycoproteins that play an important role in the survival of growing oocytes, successful fertilization and the passage of early embryos through the oviduct. The encoded preproprotein undergoes proteolytic processing to generate the mature polypeptide that is incorporated into the extracellular matrix surrounding mouse oocytes. Mice lacking the encoded protein develop defective zonae pellucidae that disrupt folliculogenesis, fertility and development. [provided by RefSeq, Sep 2016]
    Expression
    Restricted expression toward ovary adult (RPKM 15.6) See more
    Orthologs
    human all
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    Genomic context

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    See Zp2 in Genome Data Viewer
    Location:
    7 F2; 7 64.38 cM
    Exon count:
    20
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 7 NC_000073.7 (119725985..119744514, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 7 NC_000073.6 (120126762..120145291, complement)

    Chromosome 7 - NC_000073.7Genomic Context describing neighboring genes Neighboring gene dynein, axonemal, heavy chain 3 Neighboring gene ribosomal protein S9 pseudogene Neighboring gene STARR-seq mESC enhancer starr_20042 Neighboring gene lipid droplet assembly factor 1 Neighboring gene RIKEN cDNA 4930505K13 gene Neighboring gene STARR-seq mESC enhancer starr_20043 Neighboring gene ankyrin repeat and sterile alpha motif domain containing 4B

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. A heterozygous ZP2 mutation causes zona pellucida defects and female infertility in mouse and human. Liu SL, et al. iScience, 2023 Oct 20. PMID 37736051, Free PMC Article
    2. Oocyte-specific expression of mouse Zp-2: developmental regulation of the zona pellucida genes. Liang LF, et al. Mol Cell Biol, 1990 Apr. PMID 1690843, Free PMC Article
    3. Primary structure and developmental expression of Dp ZP2, a vitelline envelope glycoprotein homolog of mouse ZP2, in Discoglossus pictus, one of the oldest living Anuran species. Vaccaro MC, et al. Mol Reprod Dev, 2001 Jun. PMID 11389548
    4. Defective zonae pellucidae in Zp2-null mice disrupt folliculogenesis, fertility and development. Rankin TL, et al. Development, 2001 Apr. PMID 11245577
    5. The bioactivities of the central segment of Zp2 polypeptide. Cao Z, et al. Zygote, 2016 Oct. PMID 27193969

    See all (76) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Identification of a heterozygous variant of ZP2 as a novel cause of empty follicle syndrome in humans and mice.
      Title: Identification of a heterozygous variant of ZP2 as a novel cause of empty follicle syndrome in humans and mice.
    2. The central peptide of ZP2 (190-505) is immunogenic and contains zona pellucida-specific epitopes.
      Title: The bioactivities of the central segment of Zp2 polypeptide.
    3. ZP2(51-149) sperm-binding domain is necessary for mouse gamete recognition and penetration through the zona pellucida.
      Title: A single domain of the ZP2 zona pellucida protein mediates gamete recognition in mice and humans.
    4. Studies indicate that eggs from mZP2-/- and mZP3-/- females lack a zona pellucida (ZP), and the mice are completely infertile due to a severely reduced number of ovulated eggs in their oviducts.
      Title: Influence of the zona pellucida of the mouse egg on folliculogenesis and fertility.
    5. Sperm arylsulfatase A binds to mZP2 and mZP3 glycoproteins in a nonenzymatic manner.
      Title: Sperm arylsulfatase A binds to mZP2 and mZP3 glycoproteins in a nonenzymatic manner.
    6. Recombinant ovastacin cleaved ZP2 in native zonae pellucidae, documenting that ZP2 was a direct substrate of this metalloendoprotease.
      Title: Ovastacin, a cortical granule protease, cleaves ZP2 in the zona pellucida to prevent polyspermy.
    7. Cytoplasmic tails are necessary to prevent intracellular oligomerization while ensuring incorporation of processed ZP2 and ZP3 into the zona pellucida.
      Title: ZP2 and ZP3 cytoplasmic tails prevent premature interactions and ensure incorporation into the zona pellucida.
    8. The granulosa cells contribute more to the mZP2 mRNA synthesis than oocyte does.
      Title: Mouse granulosa cells contribute more to the mRNA synthesis of mZP2 than oocyte does.
    9. data indicate that sperm-egg recognition depends on the cleavage status of ZP2 and that binding at the surface of the zona is not sufficient to induce sperm acrosome exocytosis
      Title: Gamete recognition in mice depends on the cleavage status of an egg's zona pellucida protein.
    10. characterization of native mouse zona pellucida proteins by mass spectrometry
      Title: Structural characterization of native mouse zona pellucida proteins using mass spectrometry.
    Submit: New GeneRIF Correction See all GeneRIFs (12)

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables acrosin binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables acrosin binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables acrosin binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables identical protein binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables structural constituent of egg coat IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables structural constituent of egg coat ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables structural constituent of egg coat ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    Process Evidence Code Pubs
    involved_in binding of sperm to zona pellucida IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within binding of sperm to zona pellucida IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in binding of sperm to zona pellucida IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in prevention of polyspermy IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in prevention of polyspermy IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in collagen-containing extracellular matrix IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in collagen-containing extracellular matrix IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in egg coat IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in egg coat ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in egg coat ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    located_in endoplasmic reticulum IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in extracellular region IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in multivesicular body IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    zona pellucida sperm-binding protein 2
    Names
    zona pellucida glycoprotein ZP2
    zona pellucida protein A

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001374631.1 → NP_001361560.1  zona pellucida sperm-binding protein 2 isoform 2 preproprotein

      Status: REVIEWED

      Source sequence(s)
      AC122853
      Consensus CDS
      CCDS90322.1
      UniProtKB/TrEMBL
      Q3UX44, Q6IR45
      Related
      ENSMUSP00000147097.2, ENSMUST00000208874.2
      Conserved Domains (1) summary
      smart00241
      Location:365 → 630
      ZP; Zona pellucida (ZP) domain

    2. NM_011775.7 → NP_035905.1  zona pellucida sperm-binding protein 2 isoform 1 preproprotein

      See identical proteins and their annotated locations for NP_035905.1

      Status: REVIEWED

      Source sequence(s)
      AK135903, AK138699, DV657934
      Consensus CDS
      CCDS21792.1
      UniProtKB/Swiss-Prot
      P20239
      UniProtKB/TrEMBL
      Q6IR45
      Related
      ENSMUSP00000033207.5, ENSMUST00000033207.6
      Conserved Domains (1) summary
      smart00241
      Location:365 → 630
      ZP; Zona pellucida (ZP) domain

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000073.7 Reference GRCm39 C57BL/6J

      Range
      119725985..119744514 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_006507590.4 → XP_006507653.1  zona pellucida sperm-binding protein 2 isoform X1

      UniProtKB/TrEMBL
      Q6IR45
      Conserved Domains (1) summary
      smart00241
      Location:237 → 502
      ZP; Zona pellucida (ZP) domain
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P20239.1 GenPept UniProtKB/Swiss-Prot:P20239

    Gene LinkOut

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