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    Akap8 A kinase anchor protein 8 [ Mus musculus (house mouse) ]

    Gene ID: 56399, updated on 2-Nov-2024

    Summary

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    Official Symbol
    Akap8provided by MGI
    Official Full Name
    A kinase anchor protein 8provided by MGI
    Primary source
    MGI:MGI:1928488
    See related
    Ensembl:ENSMUSG00000024045 AllianceGenome:MGI:1928488
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    AKAP-8; AKAP95; 1200016A02Rik
    Summary
    This gene encodes a member of the A-kinase anchoring protein (AKAP) family. These proteins are characterized by their ability to bind to the R subunit of protein kinase A (PKA) and anchor the protein at different subcellular locations. This protein has been shown to regulate apoptosis and to be involved in palatogenesis. Knockdown of this gene has been associated with altered histone modifications and reduced expression of developmental genes in mouse embryonic stem cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]
    Expression
    Ubiquitous expression in CNS E11.5 (RPKM 24.6), CNS E14 (RPKM 20.6) and 28 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Akap8 in Genome Data Viewer
    Location:
    17 B1; 17 17.48 cM
    Exon count:
    16
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 17 NC_000083.7 (32522646..32540212, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 17 NC_000083.6 (32303672..32321238, complement)

    Chromosome 17 - NC_000083.7Genomic Context describing neighboring genes Neighboring gene epoxide hydrolase 3 Neighboring gene bromodomain containing 4 Neighboring gene predicted gene 4432 Neighboring gene STARR-positive B cell enhancer ABC_E11795 Neighboring gene STARR-positive B cell enhancer ABC_E5590 Neighboring gene STARR-positive B cell enhancer ABC_E955 Neighboring gene A kinase anchor protein 8-like Neighboring gene STARR-positive B cell enhancer ABC_E1502 Neighboring gene STARR-positive B cell enhancer mm9_chr17:32506256-32506557 Neighboring gene STARR-positive B cell enhancer mm9_chr17:32508595-32508896 Neighboring gene widely-interspaced zinc finger motifs Neighboring gene STARR-positive B cell enhancer ABC_E4205 Neighboring gene predicted gene, 53995

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Biophysical properties of AKAP95 protein condensates regulate splicing and tumorigenesis. Li W, et al. Nat Cell Biol, 2020 Aug. PMID 32719551, Free PMC Article
    2. DNA methylation, histone modifications and behaviour of AKAP95 during mouse oocyte growth and upon nuclear transfer of foreign chromatin into fully grown prophase oocytes. Hoffmann S, et al. Folia Biol (Krakow), 2012. PMID 23342911
    3. A-kinase-anchoring protein 95 functions as a potential carrier for the nuclear translocation of active caspase 3 through an enzyme-substrate-like association. Kamada S, et al. Mol Cell Biol, 2005 Nov. PMID 16227597, Free PMC Article
    4. Cloning and characterization of AKAP 95, a nuclear protein that associates with the regulatory subunit of type II cAMP-dependent protein kinase. Coghlan VM, et al. J Biol Chem, 1994 Mar 11. PMID 8125992
    5. Suppression of LPS-induced TNF-alpha production in macrophages by cAMP is mediated by PKA-AKAP95-p105. Wall EA, et al. Sci Signal, 2009 Jun 16. PMID 19531803, Free PMC Article

    See all (50) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Biophysical properties of AKAP95 protein condensates regulate splicing and tumorigenesis.
      Title: Biophysical properties of AKAP95 protein condensates regulate splicing and tumorigenesis.
    2. This study showed an increase in the level of global DNA methylation starting in oocytes with diameters around 60 microm, increases in histone h4, and decreases in AKAP95.
      Title: DNA methylation, histone modifications and behaviour of AKAP95 during mouse oocyte growth and upon nuclear transfer of foreign chromatin into fully grown prophase oocytes.
    3. a specific role for A kinase-anchoring protein 95 in suppressing the expression of the gene encoding tumor necrosis factor-alpha, which involved phosphorylation of p105 (also known as Nfkb1) by PKA
      Title: Suppression of LPS-induced TNF-alpha production in macrophages by cAMP is mediated by PKA-AKAP95-p105.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (3) 
    • Gene trapped (1)  1 citation
    • Targeted (1) 

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables DNA binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables NF-kappaB binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables chromatin binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables double-stranded DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables double-stranded DNA binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables histone deacetylase binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables histone deacetylase binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein kinase A regulatory subunit binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables protein kinase A regulatory subunit binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein kinase A regulatory subunit binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables zinc ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables zinc ion binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    Process Evidence Code Pubs
    involved_in cell cycle G2/M phase transition IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in cell cycle G2/M phase transition ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in cellular response to lipopolysaccharide IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in cellular response to prostaglandin E stimulus IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in innate immune response IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within mitotic chromosome condensation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within mitotic chromosome condensation ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in negative regulation of tumor necrosis factor production IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in protein transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in chromatin ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in condensed chromosome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in condensed chromosome ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in female pronucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in nuclear matrix IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nuclear matrix IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nuclear matrix ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in nucleolus IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleolus ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in nucleoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleoplasm ISO
    Inferred from Sequence Orthology
    more info
    		  
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    A-kinase anchor protein 8
    Names
    A kinase (PRKA) anchor protein 8
    A-kinase anchor protein 95 kDa

    NCBI Reference Sequences (RefSeq)

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    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001357760.1 → NP_001344689.1  A-kinase anchor protein 8 isoform 2

      Status: REVIEWED

      Source sequence(s)
      BC060170, CT033751
      Conserved Domains (1) summary
      pfam04988
      Location:273 → 433
      AKAP95; A-kinase anchoring protein 95 (AKAP95)

    2. NM_019774.5 → NP_062748.2  A-kinase anchor protein 8 isoform 1

      See identical proteins and their annotated locations for NP_062748.2

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) represents the shortest transcript and encodes the protein.
      Source sequence(s)
      AK004801, BC060170, BC060209
      Consensus CDS
      CCDS37555.1
      UniProtKB/Swiss-Prot
      Q9DBR0, Q9R0L8
      UniProtKB/TrEMBL
      Q059U9
      Related
      ENSMUSP00000002699.6, ENSMUST00000002699.7
      Conserved Domains (1) summary
      pfam04988
      Location:387 → 547
      AKAP95; A-kinase anchoring protein 95 (AKAP95)

    RNA

    1. NR_131218.1 RNA Sequence

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) uses an alternate splice site in an internal exon compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).
      Source sequence(s)
      BC060170, BC060209
      Related
      ENSMUST00000237381.2

    2. NR_131219.1 RNA Sequence

      Status: REVIEWED

      Description
      Transcript Variant: This variant (3) lacks two alternate internal exons and uses an alternate splice site in an internal exon compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).
      Source sequence(s)
      BC054433, BC060170, BC060209

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000083.7 Reference GRCm39 C57BL/6J

      Range
      32522646..32540212 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_006524665.5 → XP_006524728.1  A-kinase anchor protein 8 isoform X2

      See identical proteins and their annotated locations for XP_006524728.1

      Conserved Domains (1) summary
      pfam04988
      Location:273 → 433
      AKAP95; A-kinase anchoring protein 95 (AKAP95)

    2. XM_006524666.5 → XP_006524729.1  A-kinase anchor protein 8 isoform X2

      See identical proteins and their annotated locations for XP_006524729.1

      Conserved Domains (1) summary
      pfam04988
      Location:273 → 433
      AKAP95; A-kinase anchoring protein 95 (AKAP95)

    3. XM_006524663.5 → XP_006524726.1  A-kinase anchor protein 8 isoform X1

      Conserved Domains (1) summary
      pfam04988
      Location:362 → 522
      AKAP95; A-kinase anchoring protein 95 (AKAP95)
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9DBR0.1 GenPept UniProtKB/Swiss-Prot:Q9DBR0

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