Shank2 SH3 and multiple ankyrin repeat domains 2 [Rattus norvegicus (Norway rat)] - Gene

Summary

Symbol: Shank2provided by RGD
Full Name: SH3 and multiple ankyrin repeat domains 2provided by RGD
Primary source: RGD:628772
See related: EnsemblRapid:ENSRNOG00000050206 AllianceGenome:RGD:628772
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: CortBP1; ProSAP1; Shank2E; Spank-3
Summary: Enables SH3 domain binding activity. A structural constituent of postsynaptic density. Involved in several processes, including adult locomotory behavior; associative learning; and social behavior. Located in several cellular components, including apical plasma membrane; cell projection membrane; and growth cone. Is active in glutamatergic synapse; hippocampal mossy fiber to CA3 synapse; and postsynaptic density. Used to study autism spectrum disorder. Human ortholog(s) of this gene implicated in autistic disorder. Orthologous to human SHANK2 (SH3 and multiple ankyrin repeat domains 2). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Kidney (RPKM 67.9), Brain (RPKM 41.3) and 5 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 1q42

Exon count:: 31

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	1	NC_086019.1 (208575144..209020300)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	1	NC_051336.1 (199146210..199590962)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	1	NC_005100.4 (217149156..217593950)

Chromosome 1 - NC_086019.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Remote ischemic conditioning alleviates chronic cerebral hypoperfusion-induced cognitive decline and synaptic dysfunction via the miR-218a-5p/SHANK2 pathway.
Title: Remote ischemic conditioning alleviates chronic cerebral hypoperfusion-induced cognitive decline and synaptic dysfunction via the miR-218a-5p/SHANK2 pathway.
Dynamic Change of Shanks Gene mRNA Expression and DNA Methylation in Epileptic Rat Model and Human Patients.
Title: Dynamic Change of Shanks Gene mRNA Expression and DNA Methylation in Epileptic Rat Model and Human Patients.
Loss of scaffold proteins via knockdown of Shank1 or Shank2, but not Shank3, led to a reduction of the number but not the unitary response of AMPAR-containing synapses. Only when both Shank1 and Shank2 were knocked down, were both the number and the unitary response of active synapses reduced. This reduction was accompanied by a decrease in NMDAR-mediated synaptic response.
Title: Shank Proteins Differentially Regulate Synaptic Transmission.
Desipramine produced a significant increase of Shank2 mRNA levels 7 d after acute stress exposure.
Title: The expression of plasticity-related genes in an acute model of stress is modulated by chronic desipramine in a time-dependent manner within medial prefrontal cortex.
Upon depolarization with high K+, neither the intensity nor distribution of label for GKAP changed, but labeling intensity for Shank at the postsynaptic density increased to ~150% of controls.
Title: Differential distribution of Shank and GKAP at the postsynaptic density.
Shank2E is concentrated in the apical domain of renal PT cells, specifically binds NaP(i)-IIa via PDZ interactions, and undergoes P(i)-induced internalization.
Title: Shank2E binds NaP(i) cotransporter at the apical membrane of proximal tubule cells.
Concerted action of ProSAP/Shank and Zn(2+) is essential for the structural integrity of the postsynaptic density and an important element in synapse formation, maturation and structural plasticity.
Title: Concerted action of zinc and ProSAP/Shank in synaptogenesis and synapse maturation.
Scaffolding proteins ProSAP1/2 were found within the postsynaptic specializations of synapses within the PNS, indicating a similar molecular assembly of central and peripheral postsynapses.
Title: Proline-rich synapse-associated protein-1 and 2 (ProSAP1/Shank2 and ProSAP2/Shank3)-scaffolding proteins are also present in postsynaptic specializations of the peripheral nervous system.
Shank2 is positioned to coordinate the regulated endocytic retrieval and downregulation of Sodium-Phosphate Cotransporter Proteins Type IIa in rat renal proximal tubule cells.
Title: Shank2 redistributes with NaPilla during regulated endocytosis.
Data show that ProSAP1 is present in the olfactory cilia.
Title: Scaffolding proteins in highly purified rat olfactory cilia membranes.

Submit: New GeneRIF Correction See all GeneRIFs (15)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH138116 (e-PCR)
- UniSTS:220203

d1rat168 (e-PCR)
- UniSTS:121461

D1Wox81 (e-PCR)
- UniSTS:518785

D1Got184 (e-PCR)
- UniSTS:113285

Shank2 (e-PCR), detects polymorphism
- UniSTS:496556

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables SH3 domain binding	IPI Inferred from Physical Interaction more info	PubMed
enables ionotropic glutamate receptor binding	IBA Inferred from Biological aspect of Ancestor more info
enables ionotropic glutamate receptor binding	ISO Inferred from Sequence Orthology more info
enables ionotropic glutamate receptor binding	ISS Inferred from Sequence or Structural Similarity more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables structural constituent of postsynaptic density	IDA Inferred from Direct Assay more info	PubMed
enables structural constituent of postsynaptic density	IMP Inferred from Mutant Phenotype more info	PubMed
enables synaptic receptor adaptor activity	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in adult behavior	ISO Inferred from Sequence Orthology more info
involved_in adult behavior	ISS Inferred from Sequence or Structural Similarity more info
involved_in adult locomotory behavior	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in associative learning	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in exploration behavior	ISO Inferred from Sequence Orthology more info
involved_in exploration behavior	ISS Inferred from Sequence or Structural Similarity more info
involved_in learning	ISO Inferred from Sequence Orthology more info
involved_in learning	ISS Inferred from Sequence or Structural Similarity more info
involved_in long-term synaptic depression	ISO Inferred from Sequence Orthology more info
involved_in long-term synaptic depression	ISS Inferred from Sequence or Structural Similarity more info
involved_in long-term synaptic potentiation	ISO Inferred from Sequence Orthology more info
involved_in long-term synaptic potentiation	ISS Inferred from Sequence or Structural Similarity more info
involved_in maintenance of postsynaptic density structure	IEA Inferred from Electronic Annotation more info
involved_in memory	ISO Inferred from Sequence Orthology more info
involved_in memory	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of hippo signaling	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell population proliferation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of synaptic transmission, glutamatergic	ISO Inferred from Sequence Orthology more info
involved_in regulation of postsynapse organization	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of postsynapse organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to nutrient	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to xenobiotic stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in social behavior	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in social behavior	ISO Inferred from Sequence Orthology more info
involved_in social behavior	ISS Inferred from Sequence or Structural Similarity more info
involved_in synapse assembly	ISO Inferred from Sequence Orthology more info
involved_in synapse assembly	ISS Inferred from Sequence or Structural Similarity more info
involved_in synapse organization	IEA Inferred from Electronic Annotation more info
involved_in vocalization behavior	ISO Inferred from Sequence Orthology more info
involved_in vocalization behavior	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
located_in apical plasma membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in asymmetric synapse	IDA Inferred from Direct Assay more info	PubMed
located_in brush border membrane	IDA Inferred from Direct Assay more info	PubMed
located_in ciliary membrane	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in dendritic spine	IBA Inferred from Biological aspect of Ancestor more info
located_in dendritic spine	ISO Inferred from Sequence Orthology more info
located_in dendritic spine	ISS Inferred from Sequence or Structural Similarity more info
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
located_in growth cone	IDA Inferred from Direct Assay more info	PubMed
is_active_in hippocampal mossy fiber to CA3 synapse	IDA Inferred from Direct Assay more info	PubMed
located_in neurofilament	ISO Inferred from Sequence Orthology more info
located_in neurofilament	ISS Inferred from Sequence or Structural Similarity more info
located_in neuron projection	ISO Inferred from Sequence Orthology more info
located_in neuron projection	ISS Inferred from Sequence or Structural Similarity more info
located_in neuronal cell body	IDA Inferred from Direct Assay more info	PubMed
located_in photoreceptor inner segment	ISO Inferred from Sequence Orthology more info
located_in photoreceptor inner segment	ISS Inferred from Sequence or Structural Similarity more info
located_in photoreceptor outer segment	ISO Inferred from Sequence Orthology more info
located_in photoreceptor outer segment	ISS Inferred from Sequence or Structural Similarity more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in plasma membrane bounded cell projection	IEA Inferred from Electronic Annotation more info
is_active_in postsynaptic density	IBA Inferred from Biological aspect of Ancestor more info
is_active_in postsynaptic density	IDA Inferred from Direct Assay more info	PubMed
located_in postsynaptic density	IEA Inferred from Electronic Annotation more info
colocalizes_with postsynaptic membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in postsynaptic membrane	ISO Inferred from Sequence Orthology more info
located_in postsynaptic membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in presynaptic active zone	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: SH3 and multiple ankyrin repeat domains protein 2

Names: GKAP/SAPAP-interacting protein; SH3 and multiple ankyrin repeat domains protein 2-like; SH3/ankyrin domain gene 2; cortactin-binding protein 1; proline-rich synapse-associated protein 1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001004133.1 → NP_001004133.1 SH3 and multiple ankyrin repeat domains protein 2 isoform b

See identical proteins and their annotated locations for NP_001004133.1

Status: PROVISIONAL

Source sequence(s)

AF060116

UniProtKB/TrEMBL

A0A8L2RC58

Conserved Domains (3) summary

cd09506
Location:1185 → 1250

SAM_Shank1,2,3; SAM domain of Shank1,2,3 family proteins

smart00454
Location:1186 → 1252

SAM; Sterile alpha motif

cd00992
Location:36 → 128

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...
NM_133440.1 → NP_597684.1 SH3 and multiple ankyrin repeat domains protein 2 isoform c

See identical proteins and their annotated locations for NP_597684.1

Status: PROVISIONAL

Source sequence(s)

AJ131899

UniProtKB/TrEMBL

A0A8L2RC58

Conserved Domains (3) summary

cd09506
Location:1192 → 1257

SAM_Shank1,2,3; SAM domain of Shank1,2,3 family proteins

smart00454
Location:1193 → 1259

SAM; Sterile alpha motif

cd00992
Location:36 → 128

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...
NM_133441.2 → NP_597685.1 SH3 and multiple ankyrin repeat domains protein 2 isoform d

See identical proteins and their annotated locations for NP_597685.1

Status: PROVISIONAL

Source sequence(s)

JAXUCZ010000001

UniProtKB/TrEMBL

M0R5T5

Related

ENSRNOP00000075839.2, ENSRNOT00000092516.3

Conserved Domains (4) summary

cd09506
Location:1403 → 1468

SAM_Shank1,2,3; SAM domain of Shank1,2,3 family proteins

smart00454
Location:1404 → 1470

SAM; Sterile alpha motif

cd11983
Location:151 → 202

SH3_Shank2; Src homology 3 domain of SH3 and multiple ankyrin repeat domains protein 2

cd00992
Location:247 → 339

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...
NM_201350.1 → NP_958738.1 SH3 and multiple ankyrin repeat domains protein 2 isoform a

See identical proteins and their annotated locations for NP_958738.1

Status: PROVISIONAL

Source sequence(s)

AY298755

UniProtKB/TrEMBL

A0A8L2QYJ0

Related

ENSRNOP00000067841.4, ENSRNOT00000071741.5

Conserved Domains (8) summary

cd09506
Location:1772 → 1837

SAM_Shank1,2,3; SAM domain of Shank1,2,3 family proteins

smart00454
Location:1773 → 1839

SAM; Sterile alpha motif

cd11983
Location:530 → 581

SH3_Shank2; Src homology 3 domain of SH3 and multiple ankyrin repeat domains protein 2

cd00204
Location:225 → 351

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

cd00992
Location:626 → 718

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

pfam12796
Location:270 → 361

Ank_2; Ankyrin repeats (3 copies)

pfam16511
Location:57 → 140

FERM_f0; N-terminal or F0 domain of Talin-head FERM

sd00045
Location:230 → 261

ANK; ANK repeat [structural motif]

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086019.1 Reference GRCr8

Range

208575144..209020300

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_063274304.1 → XP_063130374.1 SH3 and multiple ankyrin repeat domains protein 2 isoform X1
XM_063274282.1 → XP_063130352.1 SH3 and multiple ankyrin repeat domains protein 2 isoform X1
XM_063274330.1 → XP_063130400.1 SH3 and multiple ankyrin repeat domains protein 2 isoform X1
XM_063274526.1 → XP_063130596.1 SH3 and multiple ankyrin repeat domains protein 2 isoform X6
XM_008760068.4 → XP_008758290.1 SH3 and multiple ankyrin repeat domains protein 2 isoform X2

UniProtKB/TrEMBL

A0A8L2QYJ0

Conserved Domains (8) summary

cd09506
Location:1782 → 1847

SAM_Shank1,2,3; SAM domain of Shank1,2,3 family proteins

smart00454
Location:1783 → 1849

SAM; Sterile alpha motif

cd11983
Location:530 → 581

SH3_Shank2; Src homology 3 domain of SH3 and multiple ankyrin repeat domains protein 2

cd00204
Location:225 → 351

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

cd00992
Location:626 → 718

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

pfam12796
Location:270 → 361

Ank_2; Ankyrin repeats (3 copies)

pfam16511
Location:57 → 140

FERM_f0; N-terminal or F0 domain of Talin-head FERM

sd00045
Location:230 → 261

ANK; ANK repeat [structural motif]
XM_063274415.1 → XP_063130485.1 SH3 and multiple ankyrin repeat domains protein 2 isoform X3
XM_063274560.1 → XP_063130630.1 SH3 and multiple ankyrin repeat domains protein 2 isoform X7
XM_063274623.1 → XP_063130693.1 SH3 and multiple ankyrin repeat domains protein 2 isoform X9
XM_063274252.1 → XP_063130322.1 SH3 and multiple ankyrin repeat domains protein 2 isoform X1
XM_063274452.1 → XP_063130522.1 SH3 and multiple ankyrin repeat domains protein 2 isoform X4
XM_063274595.1 → XP_063130665.1 SH3 and multiple ankyrin repeat domains protein 2 isoform X8
XM_063274485.1 → XP_063130555.1 SH3 and multiple ankyrin repeat domains protein 2 isoform X5
XM_017588727.3 → XP_017444216.1 SH3 and multiple ankyrin repeat domains protein 2 isoform X10

UniProtKB/TrEMBL

A0A8L2QYJ0
XM_063274364.1 → XP_063130434.1 SH3 and multiple ankyrin repeat domains protein 2 isoform X1
XM_063274697.1 → XP_063130767.1 SH3 and multiple ankyrin repeat domains protein 2 isoform X11

UniProtKB/Swiss-Prot

O70470, Q6WB19, Q9QX74, Q9QX93, Q9QZZ9, Q9WUV9, Q9WUW0, Q9WV46
XM_063274743.1 → XP_063130813.1 SH3 and multiple ankyrin repeat domains protein 2 isoform X12

Related

ENSRNOP00000075800.2, ENSRNOT00000092357.3
XM_039087396.2 → XP_038943324.1 SH3 and multiple ankyrin repeat domains protein 2 isoform X13

UniProtKB/TrEMBL

A0A8L2RC58

Conserved Domains (3) summary

cd09506
Location:1183 → 1248

SAM_Shank1,2,3; SAM domain of Shank1,2,3 family proteins

PHA03247
Location:469 → 878

PHA03247; large tegument protein UL36; Provisional

cd00992
Location:36 → 128

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...