Sh2d2a SH2 domain containing 2A [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Sh2d2aprovided by MGI
Official Full Name: SH2 domain containing 2Aprovided by MGI
Primary source: MGI:MGI:1351596
See related: Ensembl:ENSMUSG00000028071 AllianceGenome:MGI:1351596
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Lad; Ribp; Tsad
Summary: Predicted to enable protein-macromolecule adaptor activity. Acts upstream of or within T cell proliferation. Predicted to be located in plasma membrane. Predicted to be active in cytoplasm. Human ortholog(s) of this gene implicated in juvenile rheumatoid arthritis and multiple sclerosis. Orthologous to human SH2D2A (SH2 domain containing 2A). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in thymus adult (RPKM 35.7), spleen adult (RPKM 8.0) and 1 other tissue See more
Orthologs: human all
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Genomic context

Location:: 3 F1; 3 38.78 cM

Exon count:: 7

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	3	NC_000069.7 (87754070..87763116)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	3	NC_000069.6 (87846755..87855722)

Chromosome 3 - NC_000069.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

TSAd Plays a Major Role in Myo9b-Mediated Suppression of Malignant Pleural Effusion by Regulating TH1/TH17 Cell Response.
Title: TSAd Plays a Major Role in Myo9b-Mediated Suppression of Malignant Pleural Effusion by Regulating T(H)1/T(H)17 Cell Response.
The TSAd serves as a critical cell-intrinsic molecule in CD4(+)Foxp3(+) Tregs to regulate the translocation of Lck to mitochondria, cellular activation responses, and the development of immunoregulation following solid organ transplantation.
Title: T Cell-Specific Adaptor Protein Regulates Mitochondrial Function and CD4(+) T Regulatory Cell Activity In Vivo following Transplantation.
data suggest that TSAd modulates differentiation of experienced T cells possibly through polarization of CD4+ T cells towards the APC.
Title: Polarity of CD4+ T cells towards the antigen presenting cell is regulated by the Lck adapter TSAd.
These results revealed that vascular sprouting and permeability are both controlled through the VEGFR2-TSAd-c-Src signaling pathway in a subset of tissues, which may be useful in developing strategies to control tissue-specific pathological angiogenesis.
Title: The endothelial adaptor molecule TSAd is required for VEGF-induced angiogenic sprouting through junctional c-Src activation.
Sh2d2a protein deficient mice display reduced clearance of Dm157 MCMV infection in the spleen.
Title: Reduced MCMV Δm157 viral clearance in the absence of TSAd.
The kinase Itk and the adaptor TSAd change the specificity of the kinase Lck in T cells by promoting the phosphorylation of Tyr192.
Title: The kinase Itk and the adaptor TSAd change the specificity of the kinase Lck in T cells by promoting the phosphorylation of Tyr192.
Results suggest a modulatory role for T cell specific adapter protein SH2D2A in T cell mediated immune surveillance of cancer.
Title: SH2D2A modulates T cell mediated protection to a B cell derived tumor in transgenic mice.
Data show that tyrosine phosphorylation of VEGF receptor 2 (VEGFR2) facilitates binding of VEGFR2 to the Rous sarcoma (Src) homology 2-domain of T cell-specific adaptor (TSAd), which in turn regulates VEGF-induced activation of the c-Src tyrosine kinase.
Title: VEGFR2 induces c-Src signaling and vascular permeability in vivo via the adaptor protein TSAd.
results show that TSAd, particularly the SH2 domain of TSAd, is essential for the effector functions of T cells.
Title: The effector functions of mature T lymphocytes are impaired in transgenic mice expressing the SH2 domain of TSAd/Lad.
TSAd, through its interaction with both Itk and Lck, primes Itk for Lck mediated phosphorylation and thereby regulates CXCL12 induced T cell migration and actin cytoskeleton rearrangements
Title: T cell specific adapter protein (TSAd) interacts with Tec kinase ITK to promote CXCL12 induced migration of human and murine T cells.

Submit: New GeneRIF Correction See all GeneRIFs (14)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)
Targeted (1) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables SH3 domain binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-macromolecule adaptor activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within T cell proliferation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: SH2 domain-containing protein 2A

Names: Lck-associated adapter protein; Rlk/Itk-binding protein; SH2 domain protein 2A; T cell-specific adapter protein; p56Lck-associated adapter protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001025571.3 → NP_001020742.1 SH2 domain-containing protein 2A isoform 2

See identical proteins and their annotated locations for NP_001020742.1

Status: VALIDATED

Source sequence(s)

AC161454

Consensus CDS

CCDS17455.1

UniProtKB/TrEMBL

Q3TA00, Q5D0E4

Related

ENSMUSP00000029709.6, ENSMUST00000029709.7

Conserved Domains (1) summary

cd10416
Location:108 → 209

SH2_SH2D2A; Src homology 2 domain found in the SH2 domain containing protein 2A (SH2D2A)
NM_001411077.1 → NP_001398006.1 SH2 domain-containing protein 2A isoform 3

Status: VALIDATED

Source sequence(s)

AC161454
NM_021309.4 → NP_067284.1 SH2 domain-containing protein 2A isoform 1

See identical proteins and their annotated locations for NP_067284.1

Status: VALIDATED

Source sequence(s)

AC161454

Consensus CDS

CCDS38476.1

UniProtKB/Swiss-Prot

Q9JHP6, Q9QXK9

UniProtKB/TrEMBL

Q3TA00

Related

ENSMUSP00000103207.3, ENSMUST00000107581.9

Conserved Domains (1) summary

cd10416
Location:108 → 209

SH2_SH2D2A; Src homology 2 domain found in the SH2 domain containing protein 2A (SH2D2A)

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000069.7 Reference GRCm39 C57BL/6J

Range

87754070..87763116

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036163129.1 → XP_036019022.1 SH2 domain-containing protein 2A isoform X4

Conserved Domains (1) summary

cl15255
Location:108 → 154

SH2; Src homology 2 (SH2) domain
XM_017319606.3 → XP_017175095.1 SH2 domain-containing protein 2A isoform X3

UniProtKB/TrEMBL

Q3TA00

Conserved Domains (1) summary

cl15255
Location:108 → 225

SH2; Src homology 2 (SH2) domain
XM_006501554.4 → XP_006501617.1 SH2 domain-containing protein 2A isoform X1

UniProtKB/TrEMBL

Q3TA00

Conserved Domains (1) summary

cl15255
Location:108 → 225

SH2; Src homology 2 (SH2) domain