Ube2j1 ubiquitin-conjugating enzyme E2J 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ube2j1provided by MGI
Official Full Name: ubiquitin-conjugating enzyme E2J 1provided by MGI
Primary source: MGI:MGI:1926245
See related: Ensembl:ENSMUSG00000028277 AllianceGenome:MGI:1926245
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Ncube; Ubc6p; Ncube1; NCUBE-1; 0710008M05Rik; 1110030I22Rik
Summary: Predicted to enable ubiquitin conjugating enzyme activity and ubiquitin protein ligase binding activity. Acts upstream of or within ERAD pathway; regulation of cytokine production; and spermatid development. Predicted to be active in endoplasmic reticulum membrane and nucleus. Is expressed in several structures, including alimentary system; genitourinary system; hemolymphoid system; nervous system; and sensory organ. Orthologous to human UBE2J1 (ubiquitin conjugating enzyme E2 J1). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Broad expression in testis adult (RPKM 89.0), cerebellum adult (RPKM 20.0) and 27 other tissues See more
Orthologs: human all
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Genomic context

Location:: 4 A5; 4 14.61 cM

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	4	NC_000070.7 (33031380..33052367)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	4	NC_000070.6 (33031380..33052367)

Chromosome 4 - NC_000070.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

UBE2J1 is required for spermiogenesis in mice
Title: The E2 ubiquitin-conjugating enzyme UBE2J1 is required for spermiogenesis in mice.
Ube2j1 is phosphorylated in response to lipopolysaccharides in macrophages and contributes to MK2-dependent TNFalpha biosynthesis by an unknown mechanism.
Title: Endoplasmic reticulum-associated ubiquitin-conjugating enzyme Ube2j1 is a novel substrate of MK2 (MAPKAP kinase-2) involved in MK2-mediated TNFα production.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Targeted (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ubiquitin conjugating enzyme activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin conjugating enzyme activity	ISO Inferred from Sequence Orthology more info
enables ubiquitin conjugating enzyme activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables ubiquitin protein ligase binding	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in ERAD pathway	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within ERAD pathway	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within ERAD pathway	ISO Inferred from Sequence Orthology more info
involved_in ERAD pathway	ISO Inferred from Sequence Orthology more info
involved_in ERAD pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of retrograde protein transport, ER to cytosol	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of retrograde protein transport, ER to cytosol	ISO Inferred from Sequence Orthology more info
involved_in protein N-linked glycosylation via asparagine	ISO Inferred from Sequence Orthology more info
involved_in protein N-linked glycosylation via asparagine	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein polyubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of macrophage cytokine production	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within regulation of tumor necrosis factor production	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within spermatid development	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
is_active_in endoplasmic reticulum membrane	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: ubiquitin-conjugating enzyme E2 J1

Names: E2 ubiquitin-conjugating enzyme J1; non-canonical ubiquitin-conjugating enzyme 1; ubiquitin-conjugating enzyme E2, J1

NP_001342423.1

EC 2.3.2.23

NP_062532.2

EC 2.3.2.23

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001355494.1 → NP_001342423.1 ubiquitin-conjugating enzyme E2 J1 isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region. The encoded isoform (2) has the same N- and C-termini, but is shorter than isoform 1.

Source sequence(s)

AK149985, AL670464, AL772288, AW457749, BY416821, BY707310, CJ279553, DN175696

UniProtKB/TrEMBL

F6SPK0

Related

ENSMUSP00000115757.2, ENSMUST00000135924.2

Conserved Domains (1) summary

cd00195
Location:12 → 119

UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...
NM_019586.4 → NP_062532.2 ubiquitin-conjugating enzyme E2 J1 isoform 1

See identical proteins and their annotated locations for NP_062532.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AK149985, AL670464, AL772288, AW457749, BY416821, BY707310, CJ279553

Consensus CDS

CCDS18019.1

UniProtKB/Swiss-Prot

Q9DC92, Q9JJZ4

UniProtKB/TrEMBL

Q3TV38, Q3UDP8

Related

ENSMUSP00000118333.2, ENSMUST00000124992.8

Conserved Domains (1) summary

cd00195
Location:12 → 119

UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...