DCAF11 DDB1 and CUL4 associated factor 11 [Homo sapiens (human)] - Gene

Summary

Official Symbol: DCAF11provided by HGNC
Official Full Name: DDB1 and CUL4 associated factor 11provided by HGNC
Primary source: HGNC:HGNC:20258
See related: Ensembl:ENSG00000100897 MIM:613317; AllianceGenome:HGNC:20258
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: GL014; WDR23; PRO2389
Summary: This gene encodes a WD repeat-containing protein that interacts with the COP9 signalosome, a macromolecular complex that interacts with cullin-RING E3 ligases and regulates their activity by hydrolyzing cullin-Nedd8 conjugates. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2009]
Expression: Ubiquitous expression in liver (RPKM 25.7), small intestine (RPKM 25.5) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 14q12

Exon count:: 15

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	14	NC_000014.9 (24114777..24125242)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	14	NC_060938.1 (18313090..18323554)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	14	NC_000014.8 (24583986..24594451)

Chromosome 14 - NC_000014.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

DCAF11 Supports Targeted Protein Degradation by Electrophilic Proteolysis-Targeting Chimeras.
Title: DCAF11 Supports Targeted Protein Degradation by Electrophilic Proteolysis-Targeting Chimeras.
WDR23 regulates the expression of Nrf2-driven drug-metabolizing enzymes.
Title: WDR23 regulates the expression of Nrf2-driven drug-metabolizing enzymes.
TFEB activates Nrf2 by repressing its E3 ubiquitin ligase DCAF11 and promoting phosphorylation of p62.
Title: TFEB activates Nrf2 by repressing its E3 ubiquitin ligase DCAF11 and promoting phosphorylation of p62.
results support a model in which the activation of the TNF-alpha/NF-kappaB axis contributes to an increase in CRL4B(DCAF)(11) activity and a decrease in p21(Cip1) protein levels, thereby controlling cell cycle progression in human osteosarcoma cells.
Title: Activation of TNF-α/NF-κB axis enhances CRL4B(DCAF)(11) E3 ligase activity and regulates cell cycle progression in human osteosarcoma cells.
Here, the authors demonstrated that CUL4B forms an E3 ligase with RBX1 (RING-box 1), DDB1 (DNA damage binding protein 1), and DCAF11 (DDB1 and CUL4 associated factor 11) that promotes the ubiquitination of p21(Cip1) and regulates cell cycle progression in human osteosarcoma cells.
Title: CRL4B(DCAF11) E3 ligase targets p21 for degradation to control cell cycle progression in human osteosarcoma cells.
CRL4(WDR23) is required for efficient histone mRNA 3' end processing to produce mature histone mRNAs for translation. CRL4(WDR23) binds and ubiquitylates SLBP in vitro and in vivo, and this modification activates SLBP function in histone mRNA 3' end processing without affecting its protein levels.
Title: CRL4(WDR23)-Mediated SLBP Ubiquitylation Ensures Histone Supply during DNA Replication.
CRL4-DCAF11 mediates the degradation of SLBP at the end of S phase and this degradation is essential for the viability of cells.
Title: DDB1 and CUL4 associated factor 11 (DCAF11) mediates degradation of Stem-loop binding protein at the end of S phase.
ur results identify WDR23 as an alternative regulator of NRF2 proteostasis and uncover a cellular pathway that regulates NRF2 activity and capacity for cytoprotection independently of KEAP1.
Title: WDR23 regulates NRF2 independently of KEAP1.
Includes the functional characterization of a similar protein in C. elegans and comparison to the human protein.
Title: The WD40 repeat protein WDR-23 functions with the CUL4/DDB1 ubiquitin ligase to regulate nuclear abundance and activity of SKN-1 in Caenorhabditis elegans.

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Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Protein interactions

Protein	Gene	Interaction	Pubs
Vif	vif	HIV-1 Vif is identified to have a physical interaction with DDB1 and CUL4 associated factor 11 (DCAF11) in human HEK293 and/or Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses	PubMed
Vpr	vpr	HIV-1 Vpr is identified to have a physical interaction with DDB1 and CUL4 associated factor 11 (DCAF11; WDR23) in human HEK293 and/or Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses	PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D14S711E (e-PCR)
- UniSTS:151584

RH1634 (e-PCR)
- UniSTS:35189

RH11282 (e-PCR)
- UniSTS:40581

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of Cul4-RING E3 ubiquitin ligase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Cul4-RING E3 ubiquitin ligase complex	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	IDA Inferred from Direct Assay more info
located_in nucleoplasm	TAS Traceable Author Statement more info

General protein information

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Preferred Names: DDB1- and CUL4-associated factor 11

Names: WD repeat domain 23; WD repeat-containing protein 23

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001163484.2 → NP_001156956.1 DDB1- and CUL4-associated factor 11 isoform 1

See identical proteins and their annotated locations for NP_001156956.1

Status: REVIEWED

Description

Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Both variants 1 and 3 encode the same isoform (1).

Source sequence(s)

AB209073, AK074296, AK094478, BC067132, BX406971, CD644652

Consensus CDS

CCDS9610.1

UniProtKB/Swiss-Prot

B3KQ83, D3DS56, Q5D039, Q86U00, Q86U39, Q8NDN2, Q8TEB1, Q9H2J0, Q9H3A3, Q9H5C9

UniProtKB/TrEMBL

A8K9T2

Related

ENSP00000452898.1, ENST00000559115.5

Conserved Domains (2) summary

sd00039
Location:221 → 263

7WD40; WD40 repeat [structural motif]

cl25539
Location:169 → 511

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...
NM_025230.5 → NP_079506.3 DDB1- and CUL4-associated factor 11 isoform 1

See identical proteins and their annotated locations for NP_079506.3

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Both variants 1 and 3 encode the same isoform.

Source sequence(s)

AB209073, AK074296, AK094478, BP245093, BX406971

Consensus CDS

CCDS9610.1

UniProtKB/Swiss-Prot

B3KQ83, D3DS56, Q5D039, Q86U00, Q86U39, Q8NDN2, Q8TEB1, Q9H2J0, Q9H3A3, Q9H5C9

UniProtKB/TrEMBL

A8K9T2

Related

ENSP00000415556.4, ENST00000446197.8

Conserved Domains (2) summary

sd00039
Location:221 → 263

7WD40; WD40 repeat [structural motif]

cl25539
Location:169 → 511

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...
NM_181357.2 → NP_852002.1 DDB1- and CUL4-associated factor 11 isoform 2

See identical proteins and their annotated locations for NP_852002.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate in-frame segment in the 5' coding region, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1.

Source sequence(s)

AB209073, AK057636, AK094478, BX406971, DB212727

Consensus CDS

CCDS41929.1

UniProtKB/TrEMBL

A8K9T2

Related

ENSP00000380146.4, ENST00000396941.8

Conserved Domains (3) summary

COG2319
Location:143 → 505

WD40; WD40 repeat [General function prediction only]

sd00039
Location:195 → 237

7WD40; WD40 repeat [structural motif]

cl02567
Location:143 → 485

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...

RNA

NR_028099.2 RNA Sequence

Status: REVIEWED

Description

Transcript Variant: This variant (4) uses an alternate splice site in a 5' exon and lacks an internal exon, compared to variant 1. This variant is represented as non-coding because the use of the supported translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AB209073, AL136295, BP245093, BX406971

Related

ENST00000557802.5
NR_028100.1 RNA Sequence

Status: REVIEWED

Description

Transcript Variant: This variant (5) differs in its 5'-most exon and uses an alternate splice site in an internal exon, compared to variant 1. This variant is represented as non-coding because the use of the supported translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AB209073, AK094478, AK296742, AL136295, BX406971, DB003266