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    Pfkfb4 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 [ Mus musculus (house mouse) ]

    Gene ID: 270198, updated on 2-Nov-2024

    Summary

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    Official Symbol
    Pfkfb4provided by MGI
    Official Full Name
    6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4provided by MGI
    Primary source
    MGI:MGI:2687284
    See related
    Ensembl:ENSMUSG00000025648 AllianceGenome:MGI:2687284
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    C230090D14
    Summary
    Predicted to enable 6-phosphofructo-2-kinase activity and fructose-2,6-bisphosphate 2-phosphatase activity. Predicted to be involved in fructose 2,6-bisphosphate metabolic process. Predicted to be active in cytosol. Is expressed in several structures, including eye; genitourinary system; gut; nervous system; and respiratory system. Orthologous to human PFKFB4 (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4). [provided by Alliance of Genome Resources, Nov 2024]
    Expression
    Broad expression in large intestine adult (RPKM 18.1), small intestine adult (RPKM 17.4) and 25 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Pfkfb4 in Genome Data Viewer
    Location:
    9 F2; 9 59.63 cM
    Exon count:
    18
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 9 NC_000075.7 (108817336..108861302)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 9 NC_000075.6 (108988272..109032236)

    Chromosome 9 - NC_000075.7Genomic Context describing neighboring genes Neighboring gene collagen, type VII, alpha 1 Neighboring gene microRNA 711 Neighboring gene STARR-positive B cell enhancer ABC_E192 Neighboring gene urocortin 2 Neighboring gene STARR-seq mESC enhancer starr_25229 Neighboring gene STARR-positive B cell enhancer ABC_E5105 Neighboring gene shisa family member 5 Neighboring gene STARR-positive B cell enhancer ABC_E5106 Neighboring gene STARR-positive B cell enhancer ABC_E10586 Neighboring gene STARR-positive B cell enhancer ABC_E10587 Neighboring gene STARR-positive B cell enhancer ABC_E5107 Neighboring gene predicted gene, 53191 Neighboring gene three prime repair exonuclease 1

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Hypoxic activation of PFKFB4 in breast tumor microenvironment shapes metabolic and cellular plasticity to accentuate metastatic competence. Dai T, et al. Cell Rep, 2022 Dec 6. PMID 36476868, Free PMC Article
    2. PFKFB4 facilitates palbociclib resistance in oestrogen receptor-positive breast cancer by enhancing stemness. Wang S, et al. Cell Prolif, 2023 Jan. PMID 36127291, Free PMC Article
    3. Targeting the sugar metabolism of tumors with a first-in-class 6-phosphofructo-2-kinase (PFKFB4) inhibitor. Chesney J, et al. Oncotarget, 2015 Jul 20. PMID 26221874, Free PMC Article
    4. Splice isoform of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-4: expression and hypoxic regulation. Minchenko OH, et al. Mol Cell Biochem, 2005 Dec. PMID 16311927
    5. Hypoxic regulation of the 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene family (PFKFB-1-4) expression in vivo. Minchenko O, et al. FEBS Lett, 2003 Nov 20. PMID 14623077

    See all (25) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. PFKFB4 facilitates palbociclib resistance in oestrogen receptor-positive breast cancer by enhancing stemness.
      Title: PFKFB4 facilitates palbociclib resistance in oestrogen receptor-positive breast cancer by enhancing stemness.
    2. Hypoxic activation of PFKFB4 in breast tumor microenvironment shapes metabolic and cellular plasticity to accentuate metastatic competence.
      Title: Hypoxic activation of PFKFB4 in breast tumor microenvironment shapes metabolic and cellular plasticity to accentuate metastatic competence.
    3. Inhibition of PFKFB4 suppresses glycolysis and proliferation of multiple human cancer cell lines.
      Title: Targeting the sugar metabolism of tumors with a first-in-class 6-phosphofructo-2-kinase (PFKFB4) inhibitor.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (3) 
    • Targeted (2) 

    Pathways from PubChem

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    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables 6-phosphofructo-2-kinase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables 6-phosphofructo-2-kinase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables fructose-2,6-bisphosphate 2-phosphatase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables fructose-2,6-bisphosphate 2-phosphatase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables fructose-2,6-bisphosphate 2-phosphatase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    Process Evidence Code Pubs
    involved_in carbohydrate phosphorylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in fructose 2,6-bisphosphate metabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in fructose 2,6-bisphosphate metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in fructose metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  

    General protein information

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    Preferred Names
    6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4
    Names
    6PF-2-K/Fru-2,6-P2ase 4
    6PF-2-K/Fru-2,6-P2ase testis-type isozyme
    PFK/FBPase 4
    NP_766607.3
    XP_006512196.1
    XP_017168891.1
    XP_017168892.1
    XP_017168893.1
    XP_030100264.1
    XP_036010927.1
    XP_036010928.1
    XP_036010929.1
    XP_036010930.1
    XP_036010931.1
    XP_036010932.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001431581.1 → NP_001418510.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform 2

      Status: VALIDATED

      Source sequence(s)
      AC140383

    2. NM_001431582.1 → NP_001418511.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform 3

      Status: VALIDATED

      Source sequence(s)
      AC140383

    3. NM_173019.6 → NP_766607.3  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform 1

      See identical proteins and their annotated locations for NP_766607.3

      Status: VALIDATED

      Source sequence(s)
      AC140383
      Consensus CDS
      CCDS23541.1
      UniProtKB/Swiss-Prot
      Q5UD55, Q5UD56, Q5UD57, Q5Y296, Q6DTY7, Q6PFE8
      Related
      ENSMUSP00000142378.2, ENSMUST00000198140.5
      Conserved Domains (2) summary
      pfam01591
      Location:35 → 249
      6PF2K; 6-phosphofructo-2-kinase
      pfam00300
      Location:252 → 435
      His_Phos_1; Histidine phosphatase superfamily (branch 1)

    RNA

    1. NR_153429.2 RNA Sequence

      Status: VALIDATED

      Source sequence(s)
      AC140383

    2. NR_153430.2 RNA Sequence

      Status: VALIDATED

      Source sequence(s)
      AC140383

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000075.7 Reference GRCm39 C57BL/6J

      Range
      108817336..108861302
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_036155037.1 → XP_036010930.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X4

      Conserved Domains (2) summary
      pfam00300
      Location:305 → 477
      His_Phos_1; Histidine phosphatase superfamily (branch 1)
      pfam01591
      Location:24 → 302
      6PF2K; 6-phosphofructo-2-kinase

    2. XM_036155035.1 → XP_036010928.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X2

      Conserved Domains (2) summary
      pfam00300
      Location:308 → 480
      His_Phos_1; Histidine phosphatase superfamily (branch 1)
      pfam01591
      Location:27 → 305
      6PF2K; 6-phosphofructo-2-kinase

    3. XM_036155038.1 → XP_036010931.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X5

      Conserved Domains (2) summary
      pfam00300
      Location:294 → 466
      His_Phos_1; Histidine phosphatase superfamily (branch 1)
      pfam01591
      Location:13 → 291
      6PF2K; 6-phosphofructo-2-kinase

    4. XM_006512133.3 → XP_006512196.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X6

      Conserved Domains (2) summary
      pfam01591
      Location:35 → 249
      6PF2K; 6-phosphofructo-2-kinase
      pfam00300
      Location:252 → 431
      His_Phos_1; Histidine phosphatase superfamily (branch 1)

    5. XM_036155036.1 → XP_036010929.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X3

      Conserved Domains (2) summary
      pfam00300
      Location:316 → 502
      His_Phos_1; Histidine phosphatase superfamily (branch 1)
      pfam01591
      Location:30 → 313
      6PF2K; 6-phosphofructo-2-kinase

    6. XM_036155034.1 → XP_036010927.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X1

      Conserved Domains (2) summary
      pfam00300
      Location:316 → 488
      His_Phos_1; Histidine phosphatase superfamily (branch 1)
      pfam01591
      Location:30 → 313
      6PF2K; 6-phosphofructo-2-kinase

    7. XM_030244404.2 → XP_030100264.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X7

      Conserved Domains (2) summary
      pfam01591
      Location:1 → 215
      6PF2K; 6-phosphofructo-2-kinase
      pfam00300
      Location:218 → 397
      His_Phos_1; Histidine phosphatase superfamily (branch 1)

    8. XM_017313402.3 → XP_017168891.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X8

      UniProtKB/TrEMBL
      C9VZF2
      Conserved Domains (2) summary
      pfam01591
      Location:1 → 215
      6PF2K; 6-phosphofructo-2-kinase
      pfam00300
      Location:218 → 401
      His_Phos_1; Histidine phosphatase superfamily (branch 1)

    9. XM_017313404.2 → XP_017168893.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X10

      UniProtKB/TrEMBL
      C9VZF3
      Conserved Domains (2) summary
      pfam00300
      Location:61 → 244
      His_Phos_1; Histidine phosphatase superfamily (branch 1)
      cl17190
      Location:1 → 58
      NK; Nucleoside/nucleotide kinase (NK) is a protein superfamily consisting of multiple families of enzymes that share structural similarity and are functionally related to the catalysis of the reversible phosphate group transfer from nucleoside triphosphates ...

    10. XM_036155039.1 → XP_036010932.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X11

      Conserved Domains (2) summary
      pfam00300
      Location:34 → 206
      His_Phos_1; Histidine phosphatase superfamily (branch 1)
      cl17190
      Location:1 → 31
      NK; Nucleoside/nucleotide kinase (NK) is a protein superfamily consisting of multiple families of enzymes that share structural similarity and are functionally related to the catalysis of the reversible phosphate group transfer from nucleoside triphosphates ...

    11. XM_017313403.2 → XP_017168892.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform X9

      Conserved Domains (2) summary
      pfam00300
      Location:61 → 240
      His_Phos_1; Histidine phosphatase superfamily (branch 1)
      cl17190
      Location:1 → 58
      NK; Nucleoside/nucleotide kinase (NK) is a protein superfamily consisting of multiple families of enzymes that share structural similarity and are functionally related to the catalysis of the reversible phosphate group transfer from nucleoside triphosphates ...

    Suppressed Reference Sequence(s)

    The following Reference Sequences have been suppressed. Explain

    1. NM_001039215.1: Suppressed sequence

      Description
      NM_001039215.1: This RefSeq was permanently suppressed because it is a nonsense-mediated mRNA decay (NMD) candidate.

    2. NM_001039216.1: Suppressed sequence

      Description
      NM_001039216.1: This RefSeq was permanently suppressed because it is a nonsense-mediated mRNA decay (NMD) candidate.

    3. NM_001039217.1: Suppressed sequence

      Description
      NM_001039217.1: This RefSeq was removed because currently there is support for the transcript but not for the protein.
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q6DTY7.4 GenPept UniProtKB/Swiss-Prot:Q6DTY7

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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