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    AJUBA ajuba LIM protein [ Homo sapiens (human) ]

    Gene ID: 84962, updated on 28-Oct-2024

    Summary

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    Official Symbol
    AJUBAprovided by HGNC
    Official Full Name
    ajuba LIM proteinprovided by HGNC
    Primary source
    HGNC:HGNC:20250
    See related
    Ensembl:ENSG00000129474 MIM:609066; AllianceGenome:HGNC:20250
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    JUB
    Summary
    Enables alpha-catenin binding activity and transcription corepressor activity. Involved in several processes, including miRNA-mediated gene silencing by inhibition of translation; negative regulation of hippo signaling; and regulation of cellular response to hypoxia. Acts upstream of or within miRNA-mediated post-transcriptional gene silencing and positive regulation of protein-containing complex assembly. Located in several cellular components, including Golgi apparatus; P-body; and nucleoplasm. [provided by Alliance of Genome Resources, Oct 2024]
    Expression
    Broad expression in skin (RPKM 17.2), liver (RPKM 8.4) and 15 other tissues See more
    Orthologs
    mouse all
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    Genomic context

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    See AJUBA in Genome Data Viewer
    Location:
    14q11.2
    Exon count:
    9
    Annotation release Status Assembly Chr Location
    RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 14 NC_000014.9 (22971177..22982551, complement)
    RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 14 NC_060938.1 (17171795..17183169, complement)
    RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 14 NC_000014.8 (23440386..23451760, complement)

    Chromosome 14 - NC_000014.9Genomic Context describing neighboring genes Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 5596 Neighboring gene tRNA-Arg (anticodon ACG) 1-3 Neighboring gene PRMT5 divergent transcript Neighboring gene HAUS augmin like complex subunit 4 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 8155 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr14:23426503-23427082 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr14:23427083-23427661 Neighboring gene microRNA 4707 Neighboring gene H3K27ac hESC enhancer GRCh37_chr14:23444107-23444607 Neighboring gene Sharpr-MPRA regulatory region 809 Neighboring gene uncharacterized LOC124903287 Neighboring gene H3K27ac hESC enhancer GRCh37_chr14:23450809-23451774 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr14:23451775-23452740 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 5597 Neighboring gene AJUBA divergent transcript Neighboring gene chromosome 14 open reading frame 93 Neighboring gene uncharacterized LOC124903286 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 8156 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 5598 Neighboring gene H3K27ac hESC enhancer GRCh37_chr14:23477153-23477713

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

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    Related articles in PubMed

    1. Ajuba, a cytosolic LIM protein, shuttles into the nucleus and affects embryonal cell proliferation and fate decisions. Kanungo J, et al. Mol Biol Cell, 2000 Oct. PMID 11029037, Free PMC Article
    2. Knockout of Ajuba Attenuates the Growth and Migration of Hepatocellular Carcinoma Cells. Le Y, et al. Cytogenet Genome Res, 2020. PMID 33640888
    3. Ajuba: An emerging signal transducer in oncogenesis. Jia H, et al. Pharmacol Res, 2020 Jan. PMID 31740385
    4. AJUBA increases the cisplatin resistance through hippo pathway in cervical cancer. Bi L, et al. Gene, 2018 Feb 20. PMID 29126926
    5. The scaffold protein Ajuba suppresses CdGAP activity in epithelia to maintain stable cell-cell contacts. McCormack JJ, et al. Sci Rep, 2017 Aug 23. PMID 28835688, Free PMC Article

    See all (74) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. LIM domain-containing protein Ajuba inhibits chemotherapy-induced apoptosis by negatively regulating p53 stability in colorectal cancer cells.
      Title: LIM domain-containing protein Ajuba inhibits chemotherapy-induced apoptosis by negatively regulating p53 stability in colorectal cancer cells.
    2. Ajuba Overexpression Promotes Breast Cancer Chemoresistance and Glucose Uptake through TAZ-GLUT3/Survivin Pathway.
      Title: Ajuba Overexpression Promotes Breast Cancer Chemoresistance and Glucose Uptake through TAZ-GLUT3/Survivin Pathway.
    3. miR-433-3p Targets AJUBA to Inhibit Malignant Progression of Glioma.
      Title: miR-433-3p Targets AJUBA to Inhibit Malignant Progression of Glioma.
    4. Ajuba transactivates N-cadherin expression in colorectal cancer cells through interaction with Twist.
      Title: Ajuba transactivates N-cadherin expression in colorectal cancer cells through interaction with Twist.
    5. Mitophagy antagonism by ZIKV reveals Ajuba as a regulator of PINK1 signaling, PKR-dependent inflammation, and viral invasion of tissues.
      Title: Mitophagy antagonism by ZIKV reveals Ajuba as a regulator of PINK1 signaling, PKR-dependent inflammation, and viral invasion of tissues.
    6. Hsa_circ_0128846 promotes tumorigenesis of colorectal cancer by sponging hsa-miR-1184 and releasing AJUBA and inactivating Hippo/YAP signalling.
      Title: Hsa_circ_0128846 promotes tumorigenesis of colorectal cancer by sponging hsa-miR-1184 and releasing AJUBA and inactivating Hippo/YAP signalling.
    7. Super-enhancer-driven AJUBA is activated by TCF4 and involved in epithelial-mesenchymal transition in the progression of Hepatocellular Carcinoma.
      Title: Super-enhancer-driven AJUBA is activated by TCF4 and involved in epithelial-mesenchymal transition in the progression of Hepatocellular Carcinoma.
    8. Knockout of Ajuba Attenuates the Growth and Migration of Hepatocellular Carcinoma Cells.
      Title: Knockout of Ajuba Attenuates the Growth and Migration of Hepatocellular Carcinoma Cells.
    9. Ajuba: An emerging signal transducer in oncogenesis.
      Title: Ajuba: An emerging signal transducer in oncogenesis.
    10. Ajuba functions as a co-activator of SP1 to induce its target gene, and that Ajuba itself is a target genes of SP1. Ajuba/SP1 complex could form a feed forward loop to drive SP1 target gene transcription and promote cell proliferation of pancreatic cancer cells.
      Title: The LIM protein Ajuba/SP1 complex forms a feed forward loop to induce SP1 target genes and promote pancreatic cancer cell proliferation.
    Submit: New GeneRIF Correction See all GeneRIFs (34)

    Phenotypes

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    Find tests for this gene in the NIH Genetic Testing Registry (GTR)

    Review eQTL and phenotype association data in this region using PheGenI

    EBI GWAS Catalog

    Description
    Genome-wide association study of primary tooth eruption identifies pleiotropic loci associated with height and craniofacial distances.
    EBI GWAS Catalog

    Variation

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    See variants in ClinVar

    See studies and variants in dbVar

    See Variation Viewer (GRCh37.p13)

    See Variation Viewer (GRCh38)

    Genotypes

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Clone Names

    • MGC15563

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables actin filament binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables alpha-catenin binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables chromatin binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein kinase activator activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables transcription corepressor activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables transcription corepressor activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in cytoskeleton organization IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in focal adhesion assembly IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in glycerophospholipid biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in lamellipodium assembly IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in miRNA-mediated gene silencing by inhibition of translation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within miRNA-mediated post-transcriptional gene silencing IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in negative regulation of hippo signaling IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in negative regulation of hippo signaling IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in negative regulation of transcription by RNA polymerase II IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in positive regulation of canonical NF-kappaB signal transduction IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within positive regulation of protein-containing complex assembly IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in protein localization IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in regulation of DNA-templated transcription IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in regulation of cell migration IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in regulation of cellular response to hypoxia IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in response to hypoxia IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in response to hypoxia IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in wound healing, spreading of epidermal cells IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in Golgi apparatus IDA
    Inferred from Direct Assay
    more info
    		  
    is_active_in P-body IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in P-body IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in adherens junction IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cell-cell junction IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in centrosome IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		  
    located_in cytosol TAS
    Traceable Author Statement
    more info
    		  
    located_in focal adhesion IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in lamellipodium IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleoplasm IDA
    Inferred from Direct Assay
    more info
    		  
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of transcription regulator complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  

    General protein information

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    Preferred Names
    LIM domain-containing protein ajuba
    Names
    jub, ajuba homolog

    NCBI Reference Sequences (RefSeq)

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    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001289097.2 → NP_001276026.1  LIM domain-containing protein ajuba isoform 3

      See identical proteins and their annotated locations for NP_001276026.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (3) lacks multiple 3' coding exons and contains an alternate 3' terminal exon, resulting in a different 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (3) has a distinct C-terminus and is shorter than isoform 1.
      Source sequence(s)
      AA909473, AL132780, BC034968, BX109281
      UniProtKB/Swiss-Prot
      Q96IF1

    2. NM_032876.6 → NP_116265.1  LIM domain-containing protein ajuba isoform 1

      See identical proteins and their annotated locations for NP_116265.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
      Source sequence(s)
      AL132780
      Consensus CDS
      CCDS9581.1
      UniProtKB/Swiss-Prot
      A8MX18, D3DS37, Q96IF1
      Related
      ENSP00000262713.2, ENST00000262713.7
      Conserved Domains (4) summary
      PHA03418
      Location:18 → 103
      PHA03418; hypothetical E4 protein; Provisional
      cd09352
      Location:338 → 391
      LIM1_Ajuba_like; The first LIM domain of Ajuba-like proteins
      cd09355
      Location:403 → 455
      LIM2_Ajuba_like; The second LIM domain of Ajuba-like proteins
      cd09438
      Location:463 → 524
      LIM3_Ajuba_like; The third LIM domain of Ajuba-like proteins

    3. NM_198086.3 → NP_932352.1  LIM domain-containing protein ajuba isoform 2

      See identical proteins and their annotated locations for NP_932352.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1.
      Source sequence(s)
      AL132780
      Consensus CDS
      CCDS9582.1
      UniProtKB/Swiss-Prot
      Q96IF1
      Related
      ENSP00000380543.3, ENST00000397388.7
      Conserved Domains (1) summary
      cl02475
      Location:46 → 107
      LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000014.9 Reference GRCh38.p14 Primary Assembly

      Range
      22971177..22982551 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060938.1 Alternate T2T-CHM13v2.0

      Range
      17171795..17183169 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q96IF1.1 GenPept UniProtKB/Swiss-Prot:Q96IF1

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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