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    Eprs1 glutamyl-prolyl-tRNA synthetase 1 [ Rattus norvegicus (Norway rat) ]

    Gene ID: 289352, updated on 2-Nov-2024

    Summary

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    Official Symbol
    Eprs1provided by RGD
    Official Full Name
    glutamyl-prolyl-tRNA synthetase 1provided by RGD
    Primary source
    RGD:1310952
    See related
    EnsemblRapid:ENSRNOG00000002393 AllianceGenome:RGD:1310952
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Rattus norvegicus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
    Also known as
    Eprs
    Summary
    Predicted to enable several functions, including aminoacyl-tRNA ligase activity; protein homodimerization activity; and zinc ion binding activity. Predicted to be involved in several processes, including cellular response to insulin stimulus; cellular response to type II interferon; and tRNA aminoacylation for protein translation. Predicted to be located in plasma membrane. Predicted to be part of GAIT complex; aminoacyl-tRNA synthetase multienzyme complex; and ribonucleoprotein complex. Predicted to be active in cytosol. Human ortholog(s) of this gene implicated in hypomyelinating leukodystrophy 15. Orthologous to human EPRS1 (glutamyl-prolyl-tRNA synthetase 1). [provided by Alliance of Genome Resources, Nov 2024]
    Expression
    Biased expression in Liver (RPKM 829.2), Kidney (RPKM 361.3) and 9 other tissues See more
    Orthologs
    human mouse all
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    Genomic context

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    See Eprs1 in Genome Data Viewer
    Location:
    13q26
    Exon count:
    35
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCr8 (GCF_036323735.1) 13 NC_086031.1 (99431955..99503510)
    RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 13 NC_051348.1 (96901548..96971966)
    106 previous assembly Rnor_6.0 (GCF_000001895.5) 13 NC_005112.4 (103300911..103371651)

    Chromosome 13 - NC_086031.1Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC134481540 Neighboring gene isoleucyl-tRNA synthetase 2, mitochondrial Neighboring gene microRNA 215 Neighboring gene 3'(2'), 5'-bisphosphate nucleotidase 1 Neighboring gene uncharacterized LOC100911719 Neighboring gene solute carrier family 30, member 10 Neighboring gene lysophospholipase-like 1

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
    • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
    • BioProject: PRJNA238328
    • Publication: PMID 24510058
    • Analysis date: Mon Jun 6 17:44:12 2016

    Bibliography

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    Related articles in PubMed

    1. Conformational changes in human prolyl-tRNA synthetase upon binding of the substrates proline and ATP and the inhibitor halofuginone. Son J, et al. Acta Crystallogr D Biol Crystallogr, 2013 Oct. PMID 24100331
    2. ATP-directed capture of bioactive herbal-based medicine on human tRNA synthetase. Zhou H, et al. Nature, 2013 Feb 7. PMID 23263184, Free PMC Article
    3. EPRS is a critical mTORC1-S6K1 effector that influences adiposity in mice. Arif A, et al. Nature, 2017 Feb 16. PMID 28178239, Free PMC Article
    4. Noncanonical function of glutamyl-prolyl-tRNA synthetase: gene-specific silencing of translation. Sampath P, et al. Cell, 2004 Oct 15. PMID 15479637
    5. Heterotrimeric GAIT complex drives transcript-selective translation inhibition in murine macrophages. Arif A, et al. Mol Cell Biol, 2012 Dec. PMID 23071094, Free PMC Article

    See all (14) citations in PubMed

    GeneRIFs: Gene References Into Functions

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by RGD

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables GTPase binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables RNA stem-loop binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables glutamate-tRNA ligase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables identical protein binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables proline-tRNA ligase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables proline-tRNA ligase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables protein homodimerization activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables zinc ion binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    Process Evidence Code Pubs
    involved_in cellular response to insulin stimulus ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in cellular response to type II interferon ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in glutamyl-tRNA aminoacylation ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in negative regulation of translation ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in prolyl-tRNA aminoacylation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in prolyl-tRNA aminoacylation ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in regulation of long-chain fatty acid import into cell ISO
    Inferred from Sequence Orthology
    more info
    		  
    Component Evidence Code Pubs
    part_of GAIT complex ISO
    Inferred from Sequence Orthology
    more info
    		  
    part_of aminoacyl-tRNA synthetase multienzyme complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of aminoacyl-tRNA synthetase multienzyme complex ISO
    Inferred from Sequence Orthology
    more info
    		  
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytoplasm ISO
    Inferred from Sequence Orthology
    more info
    		  
    is_active_in cytosol ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in cytosol ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in plasma membrane ISO
    Inferred from Sequence Orthology
    more info
    		  
    part_of ribonucleoprotein complex ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    bifunctional glutamate/proline--tRNA ligase
    Names
    bifunctional aminoacyl-tRNA synthetase
    proline--tRNA ligase-like
    NP_001019409.1
    XP_006250487.1
    XP_006250488.1
    XP_063128251.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001024238.1 → NP_001019409.1  bifunctional glutamate/proline--tRNA ligase isoform 1

      See identical proteins and their annotated locations for NP_001019409.1

      Status: PROVISIONAL

      Source sequence(s)
      AY383705
      UniProtKB/TrEMBL
      A0A8I5ZY25, Q6TXE9
      Related
      ENSRNOP00000033323.4, ENSRNOT00000038264.6
      Conserved Domains (9) summary
      cd10309
      Location:92 → 175
      GST_C_GluProRS_N; Glutathione S-transferase C-terminal-like, alpha helical domain of bifunctional Glutamyl-Prolyl-tRNA synthetase
      PLN02907
      Location:30 → 685
      PLN02907; glutamate-tRNA ligase
      PRK08661
      Location:993 → 1486
      PRK08661; prolyl-tRNA synthetase; Provisional
      cd00778
      Location:997 → 1260
      ProRS_core_arch_euk; Prolyl-tRNA synthetase (ProRS) class II core catalytic domain. ProRS is a homodimer. It is responsible for the attachment of proline to the 3' OH group of ribose of the appropriate tRNA. This domain is primarily responsible for ATP-dependent formation of ...
      cd00862
      Location:1266 → 1486
      ProRS_anticodon_zinc; ProRS Prolyl-anticodon binding domain, long version found predominantly in eukaryotes and archaea. ProRS belongs to class II aminoacyl-tRNA synthetases (aaRS). This alignment contains the anticodon binding domain, which is responsible for specificity in ...
      cd00936
      Location:800 → 849
      WEPRS_RNA; WEPRS_RNA binding domain. This short RNA-binding domain is found in several higher eukaryote aminoacyl-tRNA synthetases (aaRSs). It is found in multiple copies in eukaryotic bifunctional glutamyl-prolyl-tRNA synthetases (EPRS) in a region that separates ...
      pfam00458
      Location:727 → 779
      WHEP-TRS; WHEP-TRS domain
      pfam03950
      Location:478 → 655
      tRNA-synt_1c_C; tRNA synthetases class I (E and Q), anti-codon binding domain
      cl00015
      Location:215 → 476
      nt_trans; nucleotidyl transferase superfamily

    2. NM_001429565.1 → NP_001416494.1  bifunctional glutamate/proline--tRNA ligase isoform 2

      Status: VALIDATED

      Source sequence(s)
      JAXUCZ010000013
      UniProtKB/TrEMBL
      A0A8I6GJ92

    3. NM_001429566.1 → NP_001416495.1  bifunctional glutamate/proline--tRNA ligase isoform 3

      Status: VALIDATED

      Source sequence(s)
      JAXUCZ010000013

    RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCr8

    Genomic

    1. NC_086031.1 Reference GRCr8

      Range
      99431955..99503510
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_006250425.5 → XP_006250487.1  bifunctional glutamate/proline--tRNA ligase isoform X1

      Related
      ENSRNOP00000090936.1, ENSRNOT00000116204.2
      Conserved Domains (9) summary
      cd10309
      Location:74 → 157
      GST_C_GluProRS_N; Glutathione S-transferase C-terminal-like, alpha helical domain of bifunctional Glutamyl-Prolyl-tRNA synthetase
      PLN02907
      Location:6 → 718
      PLN02907; glutamate-tRNA ligase
      PRK08661
      Location:1026 → 1519
      PRK08661; prolyl-tRNA synthetase; Provisional
      cd00778
      Location:1030 → 1293
      ProRS_core_arch_euk; Prolyl-tRNA synthetase (ProRS) class II core catalytic domain. ProRS is a homodimer. It is responsible for the attachment of proline to the 3' OH group of ribose of the appropriate tRNA. This domain is primarily responsible for ATP-dependent formation of ...
      cd00862
      Location:1299 → 1519
      ProRS_anticodon_zinc; ProRS Prolyl-anticodon binding domain, long version found predominantly in eukaryotes and archaea. ProRS belongs to class II aminoacyl-tRNA synthetases (aaRS). This alignment contains the anticodon binding domain, which is responsible for specificity in ...
      cd00936
      Location:833 → 882
      WEPRS_RNA; WEPRS_RNA binding domain. This short RNA-binding domain is found in several higher eukaryote aminoacyl-tRNA synthetases (aaRSs). It is found in multiple copies in eukaryotic bifunctional glutamyl-prolyl-tRNA synthetases (EPRS) in a region that separates ...
      pfam00458
      Location:760 → 812
      WHEP-TRS; WHEP-TRS domain
      pfam00749
      Location:197 → 498
      tRNA-synt_1c; tRNA synthetases class I (E and Q), catalytic domain
      pfam03950
      Location:511 → 688
      tRNA-synt_1c_C; tRNA synthetases class I (E and Q), anti-codon binding domain

    2. XM_006250426.5 → XP_006250488.1  bifunctional glutamate/proline--tRNA ligase isoform X2

      Related
      ENSRNOP00000071057.2, ENSRNOT00000085214.3
      Conserved Domains (9) summary
      cd10309
      Location:74 → 157
      GST_C_GluProRS_N; Glutathione S-transferase C-terminal-like, alpha helical domain of bifunctional Glutamyl-Prolyl-tRNA synthetase
      PLN02907
      Location:6 → 711
      PLN02907; glutamate-tRNA ligase
      PRK08661
      Location:1019 → 1512
      PRK08661; prolyl-tRNA synthetase; Provisional
      cd00778
      Location:1023 → 1286
      ProRS_core_arch_euk; Prolyl-tRNA synthetase (ProRS) class II core catalytic domain. ProRS is a homodimer. It is responsible for the attachment of proline to the 3' OH group of ribose of the appropriate tRNA. This domain is primarily responsible for ATP-dependent formation of ...
      cd00862
      Location:1292 → 1512
      ProRS_anticodon_zinc; ProRS Prolyl-anticodon binding domain, long version found predominantly in eukaryotes and archaea. ProRS belongs to class II aminoacyl-tRNA synthetases (aaRS). This alignment contains the anticodon binding domain, which is responsible for specificity in ...
      cd00936
      Location:826 → 875
      WEPRS_RNA; WEPRS_RNA binding domain. This short RNA-binding domain is found in several higher eukaryote aminoacyl-tRNA synthetases (aaRSs). It is found in multiple copies in eukaryotic bifunctional glutamyl-prolyl-tRNA synthetases (EPRS) in a region that separates ...
      pfam00458
      Location:753 → 805
      WHEP-TRS; WHEP-TRS domain
      pfam00749
      Location:197 → 502
      tRNA-synt_1c; tRNA synthetases class I (E and Q), catalytic domain
      pfam03950
      Location:504 → 681
      tRNA-synt_1c_C; tRNA synthetases class I (E and Q), anti-codon binding domain

    3. XM_063272181.1 → XP_063128251.1  bifunctional glutamate/proline--tRNA ligase isoform X3

    Nucleotide Protein Strain
    Heading Accession and Version

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