Ddit3 DNA-damage inducible transcript 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ddit3provided by MGI
Official Full Name: DNA-damage inducible transcript 3provided by MGI
Primary source: MGI:MGI:109247
See related: Ensembl:ENSMUSG00000025408 AllianceGenome:MGI:109247
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: chop; CHOP10; CHOP-10; gadd153; AltDDIT3
Summary: Enables several functions, including DNA-binding transcription factor binding activity; protein heterodimerization activity; and transcription regulator activator activity. Involved in several processes, including blood vessel development; intracellular signal transduction; and regulation of transcription by RNA polymerase II. Acts upstream of or within ER overload response; negative regulation of fat cell differentiation; and sensory perception of sound. Located in late endosome and nucleus. Part of CHOP-C/EBP complex. Is expressed in several structures, including adipose tissue; alimentary system; genitourinary system; nervous system; and sensory organ. Human ortholog(s) of this gene implicated in myxoid liposarcoma. Orthologous to human DDIT3 (DNA damage inducible transcript 3). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in testis adult (RPKM 13.2), ovary adult (RPKM 9.0) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 10 D3; 10 74.5 cM

Exon count:: 4

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	10	NC_000076.7 (127126662..127132160)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	10	NC_000076.6 (127290793..127296291)

Chromosome 10 - NC_000076.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

DDIT3 aggravates TMJOA cartilage degradation via Nrf2/HO-1/NLRP3-mediated autophagy.
Title: DDIT3 aggravates TMJOA cartilage degradation via Nrf2/HO-1/NLRP3-mediated autophagy.
Transcription factor DDIT3 is a potential driver in pancreatic cancer.
Title: Transcription factor DDIT3 is a potential driver in pancreatic cancer.
Study of ATF4/CHOP axis-mediated mitochondrial unfolded protein response in neuronal apoptosis induced by methylmercury.
Title: Study of ATF4/CHOP axis-mediated mitochondrial unfolded protein response in neuronal apoptosis induced by methylmercury.
DDIT3 deficiency accelerates bone remodeling during bone healing by enhancing osteoblast and osteoclast differentiation through ULK1-mediated autophagy.
Title: DDIT3 deficiency accelerates bone remodeling during bone healing by enhancing osteoblast and osteoclast differentiation through ULK1-mediated autophagy.
C/EBPalpha-p30 confers AML cell susceptibility to the terminal unfolded protein response and resistance to Venetoclax by activating DDIT3 transcription.
Title: C/EBPα-p30 confers AML cell susceptibility to the terminal unfolded protein response and resistance to Venetoclax by activating DDIT3 transcription.
A beneficial adaptive role for CHOP in driving cell fate selection during ER stress.
Title: A beneficial adaptive role for CHOP in driving cell fate selection during ER stress.
ALKBH5 protects against stroke by reducing endoplasmic reticulum stress-dependent inflammation injury via the STAT5/PERK/EIF2alpha/CHOP signaling pathway in an m[6]A-YTHDF1-dependent manner.
Title: ALKBH5 protects against stroke by reducing endoplasmic reticulum stress-dependent inflammation injury via the STAT5/PERK/EIF2α/CHOP signaling pathway in an m(6)A-YTHDF1-dependent manner.
DNA damage-inducible transcript 3 deficiency promotes bone resorption in murine periodontitis models.
Title: DNA damage-inducible transcript 3 deficiency promotes bone resorption in murine periodontitis models.
DDIT3 aggravates pulpitis by modulating M1 polarization through EGR1 in macrophages.
Title: DDIT3 aggravates pulpitis by modulating M1 polarization through EGR1 in macrophages.
Opposite modulation of functional recovery following contusive spinal cord injury in mice with oligodendrocyte-selective deletions of Atf4 and Chop/Ddit3.
Title: Opposite modulation of functional recovery following contusive spinal cord injury in mice with oligodendrocyte-selective deletions of Atf4 and Chop/Ddit3.

Submit: New GeneRIF Correction See all GeneRIFs (196)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (4)
Targeted (7) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

X67083 (e-PCR)
- UniSTS:160053

NoName (e-PCR)
- UniSTS:225335

PMC186318P1 (e-PCR)
- UniSTS:271695

PMC152658P2 (e-PCR)
- UniSTS:271239

Ddit3 (e-PCR), detects polymorphism
- UniSTS:525963

PMC125041P1 (e-PCR)
- UniSTS:270481

PMC150726P4 (e-PCR)
- UniSTS:271076

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
contributes_to DNA binding	ISO Inferred from Sequence Orthology more info
enables DNA binding	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription activator activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-binding transcription activator activity, RNA polymerase II-specific	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription activator activity, RNA polymerase II-specific	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription factor activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables DNA-binding transcription factor activity	ISO Inferred from Sequence Orthology more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info
enables RNA polymerase II-specific DNA-binding transcription factor binding	IPI Inferred from Physical Interaction more info	PubMed
enables RNA polymerase II-specific DNA-binding transcription factor binding	ISO Inferred from Sequence Orthology more info
enables cAMP response element binding protein binding	IDA Inferred from Direct Assay more info	PubMed
enables cAMP response element binding protein binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables leucine zipper domain binding	IEA Inferred from Electronic Annotation more info
enables leucine zipper domain binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein heterodimerization activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein heterodimerization activity	IDA Inferred from Direct Assay more info	PubMed
enables protein heterodimerization activity	IPI Inferred from Physical Interaction more info	PubMed
enables protein heterodimerization activity	ISO Inferred from Sequence Orthology more info
enables protein homodimerization activity	IEA Inferred from Electronic Annotation more info
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
contributes_to sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info
enables transcription cis-regulatory region binding	IDA Inferred from Direct Assay more info	PubMed
enables transcription cis-regulatory region binding	ISO Inferred from Sequence Orthology more info
enables transcription corepressor activity	IEA Inferred from Electronic Annotation more info
enables transcription corepressor activity	ISO Inferred from Sequence Orthology more info
enables transcription regulator activator activity	IDA Inferred from Direct Assay more info	PubMed
enables transcription regulator inhibitor activity	IDA Inferred from Direct Assay more info	PubMed
enables transcription regulator inhibitor activity	IGI Inferred from Genetic Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in ER overload response	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within ER overload response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ER overload response	ISO Inferred from Sequence Orthology more info
involved_in HRI-mediated signaling	IEA Inferred from Electronic Annotation more info
involved_in Wnt signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in anterior/posterior axis specification	IDA Inferred from Direct Assay more info	PubMed
involved_in apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in artery development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in blood vessel maturation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cell redox homeostasis	ISO Inferred from Sequence Orthology more info
involved_in endoplasmic reticulum unfolded protein response	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within endoplasmic reticulum unfolded protein response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of protein localization to mitochondrion	IDA Inferred from Direct Assay more info	PubMed
involved_in gene expression	IGI Inferred from Genetic Interaction more info	PubMed
involved_in integrated stress response signaling	ISO Inferred from Sequence Orthology more info
involved_in intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress	IBA Inferred from Biological aspect of Ancestor more info
involved_in intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress	ISO Inferred from Sequence Orthology more info
involved_in intrinsic apoptotic signaling pathway in response to nitrosative stress	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of DNA-binding transcription factor activity	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of canonical Wnt signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of cold-induced thermogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of determination of dorsal identity	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of determination of dorsal identity	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of fat cell differentiation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of interleukin-17 production	IEA Inferred from Electronic Annotation more info
acts_upstream_of negative regulation of interleukin-17 production	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of interleukin-4 production	IEA Inferred from Electronic Annotation more info
acts_upstream_of negative regulation of interleukin-4 production	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of myoblast differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within negative regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of transcription by RNA polymerase II	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of type II interferon production	IEA Inferred from Electronic Annotation more info
acts_upstream_of negative regulation of type II interferon production	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of DNA-binding transcription factor activity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of autophagy in response to ER overload	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of interleukin-8 production	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of interleukin-8 production	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of intrinsic apoptotic signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of intrinsic apoptotic signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of neuron apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of neuron apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of neuron apoptotic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription by RNA polymerase II	NAS Non-traceable Author Statement more info	PubMed
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IEA Inferred from Electronic Annotation more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in regulation of autophagy	ISO Inferred from Sequence Orthology more info
involved_in regulation of autophagy	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of cell cycle	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in release of sequestered calcium ion into cytosol	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to endoplasmic reticulum stress	IDA Inferred from Direct Assay more info	PubMed
involved_in response to endoplasmic reticulum stress	ISO Inferred from Sequence Orthology more info
involved_in response to platelet-derived growth factor	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to starvation	IDA Inferred from Direct Assay more info	PubMed
involved_in response to unfolded protein	ISO Inferred from Sequence Orthology more info
involved_in response to wounding	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within sensory perception of sound	IGI Inferred from Genetic Interaction more info	PubMed
involved_in vascular associated smooth muscle cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in vascular associated smooth muscle cell proliferation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of CHOP-ATF3 complex	IBA Inferred from Biological aspect of Ancestor more info
part_of CHOP-ATF3 complex	IEA Inferred from Electronic Annotation more info
part_of CHOP-ATF3 complex	ISO Inferred from Sequence Orthology more info
part_of CHOP-ATF4 complex	IBA Inferred from Biological aspect of Ancestor more info
part_of CHOP-ATF4 complex	IEA Inferred from Electronic Annotation more info
part_of CHOP-ATF4 complex	ISO Inferred from Sequence Orthology more info
part_of CHOP-C/EBP complex	IBA Inferred from Biological aspect of Ancestor more info
part_of CHOP-C/EBP complex	IPI Inferred from Physical Interaction more info	PubMed
part_of CHOP-C/EBP complex	ISO Inferred from Sequence Orthology more info
part_of RNA polymerase II transcription regulator complex	IPI Inferred from Physical Interaction more info	PubMed
part_of RNA polymerase II transcription regulator complex	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in late endosome	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	TAS Traceable Author Statement more info
located_in nucleus	IC Inferred by Curator more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in nucleus	NAS Non-traceable Author Statement more info	PubMed
part_of protein-DNA complex	IEA Inferred from Electronic Annotation more info
part_of protein-DNA complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: DNA damage-inducible transcript 3 protein

Names: C/EBP homoologous protein 10; C/EBP zeta; CCAAT/enhancer-binding protein homologous protein; DDIT-3; alternative DDIT3 proteins; c/EBP-homologous protein 10; growth arrest and DNA-damage-inducible protein GADD153

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001290183.2 → NP_001277112.1 DNA damage-inducible transcript 3 protein

See identical proteins and their annotated locations for NP_001277112.1

Status: VALIDATED

Source sequence(s)

AC144852

Consensus CDS

CCDS24236.1

UniProtKB/Swiss-Prot

P35639, Q91YW9

UniProtKB/TrEMBL

Q3V405

Related

ENSMUSP00000118339.2, ENSMUST00000139091.2

Conserved Domains (2) summary

smart00338
Location:100 → 160

BRLZ; basic region leucin zipper

cd14686
Location:102 → 152

bZIP; coiled coil [structural motif]
NM_001428453.1 → NP_001415382.1 DNA damage-inducible transcript 3 protein

Status: VALIDATED

Source sequence(s)

AC144852

UniProtKB/Swiss-Prot

P35639, Q91YW9
NM_001428454.1 → NP_001415383.1 DNA damage-inducible transcript 3 protein

Status: VALIDATED

Source sequence(s)

AC144852

UniProtKB/Swiss-Prot

P35639, Q91YW9
NM_007837.5 → NP_031863.3 DNA damage-inducible transcript 3 protein

See identical proteins and their annotated locations for NP_031863.3

Status: VALIDATED

Source sequence(s)

AC144852

Consensus CDS

CCDS24236.1

UniProtKB/Swiss-Prot

P35639, Q91YW9

UniProtKB/TrEMBL

Q3V405

Related

ENSMUSP00000026475.9, ENSMUST00000026475.15

Conserved Domains (2) summary

smart00338
Location:100 → 160

BRLZ; basic region leucin zipper

cd14686
Location:102 → 152

bZIP; coiled coil [structural motif]

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000076.7 Reference GRCm39 C57BL/6J

Range

127126662..127132160

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006513197.4 → XP_006513260.1 DNA damage-inducible transcript 3 protein isoform X2

Conserved Domains (2) summary

smart00338
Location:100 → 160

BRLZ; basic region leucin zipper

cd14686
Location:102 → 152

bZIP; coiled coil [structural motif]
XM_030244867.1 → XP_030100727.1 DNA damage-inducible transcript 3 protein isoform X1

UniProtKB/TrEMBL

Q3V405

Conserved Domains (2) summary

smart00338
Location:114 → 174

BRLZ; basic region leucin zipper

cd14686
Location:116 → 166

bZIP; coiled coil [structural motif]