Sprn shadow of prion protein [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Sprnprovided by MGI
Official Full Name: shadow of prion proteinprovided by MGI
Primary source: MGI:MGI:3582583
See related: Ensembl:ENSMUSG00000045733 AllianceGenome:MGI:3582583
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: C630041J07
Summary: Enables nucleic acid binding activity. Acts upstream of or within protein import into nucleus. Located in several cellular components, including cytosol; nucleolus; and vesicle. Is expressed in cerebellum; embryo; and olfactory epithelium. Orthologous to human SPRN (shadow of prion protein). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in cortex adult (RPKM 34.6), frontal lobe adult (RPKM 31.0) and 5 other tissues See more
Orthologs: human all
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Genomic context

Location:: 7 F4; 7 85.17 cM

Exon count:: 2

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	7	NC_000073.7 (139730541..139734572, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	7	NC_000073.6 (140150628..140154659, complement)

Chromosome 7 - NC_000073.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Co-invalidation of Prnp and Sprn in FVB/N mice affects reproductive performances and highlight complex biological relationship between PrP and Shadoo.
Title: Co-invalidation of Prnp and Sprn in FVB/N mice affects reproductive performances and highlight complex biological relationship between PrP and Shadoo.
The Prion-like protein Shadoo is involved in mouse embryonic and mammary development and differentiation.
Title: The Prion-like protein Shadoo is involved in mouse embryonic and mammary development and differentiation.
These data are indicative of a physiological pathway to access Sho functions in the nucleus under conditions of impaired proteasomal activity.
Title: Proteasomal Inhibition Redirects the PrP-Like Shadoo Protein to the Nucleus.
Data suggest that expression of shadoo in neuron or hepatocyte cell lines induces same toxic phenotype of drug hypersensitivity as prion protein/PrP(-central region); this effect is counteracted by co-expression of PrP-wild-type.
Title: Expression of the Prion Protein Family Member Shadoo Causes Drug Hypersensitivity That Is Diminished by the Coexpression of the Wild Type Prion Protein.
No significant differences in survival, the incubation period of prion disease or other disease features were observed between Sho mutant and WT mice
Title: Unchanged survival rates of Shadoo knockout mice after infection with mouse-adapted scrapie.
These observations suggest that Shadoo can affect the prion replication process by (i) acting as a holdase and (ii) interfering with the dominant-negative inhibitor effect of the C1 fragment.
Title: Interaction between Shadoo and PrP Affects the PrP-Folding Pathway.
Data suggest that glycosylation status of the prion protein and yet-to-be-dentified proteases modulate internal C1 and C2 endoproteolysis of Shadoo (shadow of prion protein) and Doppel (doppelganger prion) in mouse neurons.
Title: Endoproteolytic processing of the mammalian prion glycoprotein family.
binding of Sho to anionic liposomes has disruptive effect on integrity of lipid bilayer leading to formation of supramolecular lipid-protein complexes. prion diseases might depend on the oligomerization state of Sho and on these lipoprotein assembles.
Title: Shadoo binds lipid membranes and undergoes aggregation and fibrillization.
Sho is not pi, or, given the accumulating data for many activities for PrP (C) , the pi hypothesis invoking a discrete signaling pathway to maintain neuronal viability is no longer tenable.
Title: Shadoo/PrP (Sprn(0/0) /Prnp(0/0) ) double knockout mice: more than zeroes.
Here we report that Shadoo (Sho)-a member of the prion protein superfamily-is also found in the nucleus of several neural and non-neural cell lines as visualized by using an YFP-Sho construct
Title: The highly conserved, N-terminal (RXXX)8 motif of mouse Shadoo mediates nuclear accumulation.

Submit: New GeneRIF Correction See all GeneRIFs (19)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2) 1 citation
Targeted (2) 1 citation

General gene information

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Markers

NoName (e-PCR)
- UniSTS:235801

UniSTS:235806 (e-PCR)
- UniSTS:235806

Sprn (e-PCR), detects polymorphism
- UniSTS:527193

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables nucleic acid binding	IBA Inferred from Biological aspect of Ancestor more info
enables nucleic acid binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in protein import into nucleus	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within protein import into nucleus	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
located_in nucleolus	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in side of membrane	IEA Inferred from Electronic Annotation more info
located_in vesicle	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: shadow of prion protein

Names: Shadoo; protein shadoo

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.