Zfp521 zinc finger protein 521 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Zfp521provided by MGI
Official Full Name: zinc finger protein 521provided by MGI
Primary source: MGI:MGI:95459
See related: Ensembl:ENSMUSG00000024420 AllianceGenome:MGI:95459
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Evi3; Znf521; B930086A16Rik
Summary: Predicted to enable protein domain specific binding activity. Acts upstream of or within neuron fate commitment. Predicted to be located in nucleoplasm. Predicted to be active in nucleus. Is expressed in several structures, including bone; branchial arch; central nervous system; embryo mesenchyme; and genitourinary system. Used to study schizophrenia. Orthologous to human ZNF521 (zinc finger protein 521). [provided by Alliance of Genome Resources, Oct 2024]
Expression: Broad expression in limb E14.5 (RPKM 13.2), cerebellum adult (RPKM 10.0) and 16 other tissues See more
Orthologs: human all
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Genomic context

Location:: 18 A1; 18 7.68 cM

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	18	NC_000084.7 (13820070..14105812, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	18	NC_000084.6 (13687013..13972749, complement)

Chromosome 18 - NC_000084.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

SIAH2 is Expressed in Adipocyte Precursor Cells and Interacts with EBF1 and ZFP521 to Promote Adipogenesis.
Title: SIAH2 is Expressed in Adipocyte Precursor Cells and Interacts with EBF1 and ZFP521 to Promote Adipogenesis.
Zfp521 SUMOylation facilities erythroid hematopoietic reconstitution under stress.
Title: Zfp521 SUMOylation facilities erythroid hematopoietic reconstitution under stress.
zinc-finger nuclear protein Zfp521 alone is sufficient for converting the adult mouse brain-derived astrocytes into induced neural stem cells.
Title: Generation of neural stem cells from adult astrocytes by using a single reprogramming factor.
Results suggest that the lack of ZFP521 upregulates the expression of DBH, which leads to a decrease in the DA level and an increase in the NA level in the brain, resulting in abnormal behaviors.
Title: Lack of zinc finger protein 521 upregulates dopamine β-hydroxylase expression in the mouse brain, leading to abnormal behavior.
Report a novel role for ZFP521 in regulating the earliest stages of hematopoiesis and lymphoid cell development via a cell-extrinsic mechanism. Mice with inactivated Zfp521 genes (Zfp521(-/-)) possess reduced frequencies and numbers of hematopoietic stem and progenitor cells, common lymphoid progenitors, and B and T cell precursors.
Title: Zinc Finger Protein 521 Regulates Early Hematopoiesis through Cell-Extrinsic Mechanisms in the Bone Marrow Microenvironment.
The pre-B-cell receptor (pre-BCR) signaling molecules BLNK, BTK and BANK1 were positively regulated by the ZFP521 gene, leading to enhancement of the pre-BCR signaling pathway.
Title: ZFP521 contributes to pre-B-cell lymphomagenesis through modulation of the pre-B-cell receptor signaling pathway.
these results identify ZNF521/ZFP521 as a critical regulator of HSC function, which facilitates MLL-AF9-mediated leukemic disease in mice.
Title: ZFP521 regulates murine hematopoietic stem cell function and facilitates MLL-AF9 leukemogenesis in mouse and human cells.
links between dysregulated Zfp521 and B-cell survival
Title: Zfp521 promotes B-cell viability and cyclin D1 gene expression in a B cell culture system.
Here we demonstrate that the transcription factors SPI1 (PU.1) and HOXC13 synergistically regulate Zfp521 expression, and identify the regions of the Zfp521 promoter required for this transcriptional activity. We also show that SPI1 and HOXC13 activate Zfp521 in a dose-dependent manner.
Title: Transcriptional regulation of the proto-oncogene Zfp521 by SPI1 (PU.1) and HOXC13.
Data suggest a facilitated approach for establishing human neural stem cells (NSCs) through zinc finger protein 521 (Zfp521)-driven conversion of fibroblasts.
Title: Conversion of Human Fibroblasts to Stably Self-Renewing Neural Stem Cells with a Single Zinc-Finger Transcription Factor.

Submit: New GeneRIF Correction See all GeneRIFs (26)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (5) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D18Mit32 (e-PCR), detects polymorphism
- UniSTS:128258

BE107765 (e-PCR)
- UniSTS:261747

26.MHAa56g2.seq (e-PCR)
- UniSTS:160714

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein domain specific binding	IEA Inferred from Electronic Annotation more info
enables protein domain specific binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within neuron fate commitment	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	TAS Traceable Author Statement more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	ISO Inferred from Sequence Orthology more info	PubMed

General protein information

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Preferred Names: zinc finger protein 521

Names: ecotropic viral integration site 3 protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001360804.1 → NP_001347733.1 zinc finger protein 521 isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.

Source sequence(s)

AC101651, AC101682, AC104328, AC108404

Conserved Domains (3) summary

COG5048
Location:83 → 236

COG5048; FOG: Zn-finger [General function prediction only]

COG5236
Location:785 → 964

COG5236; Uncharacterized conserved protein, contains RING Zn-finger [General function prediction only]

sd00017
Location:120 → 140

ZF_C2H2; C2H2 Zn finger [structural motif]
NM_145492.4 → NP_663467.1 zinc finger protein 521 isoform 1

See identical proteins and their annotated locations for NP_663467.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longer isoform (1).

Source sequence(s)

AC101651, AC101682, AC104328, AC108404

Consensus CDS

CCDS37740.1

UniProtKB/Swiss-Prot

Q6KAS7, Q8BIF5, Q8BV21, Q8CIQ2, Q8VDS6

Related

ENSMUSP00000025288.8, ENSMUST00000025288.9

Conserved Domains (2) summary

COG5048
Location:83 → 236

COG5048; FOG: Zn-finger [General function prediction only]

sd00017
Location:120 → 140

ZF_C2H2; C2H2 Zn finger [structural motif]

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000084.7 Reference GRCm39 C57BL/6J

Range

13820070..14105812 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036161092.1 → XP_036016985.1 zinc finger protein 521 isoform X2

Conserved Domains (2) summary

COG5236
Location:565 → 744

COG5236; Uncharacterized conserved protein, contains RING Zn-finger [General function prediction only]

sd00017
Location:416 → 436

ZF_C2H2; C2H2 Zn finger [structural motif]
XM_006525819.4 → XP_006525882.1 zinc finger protein 521 isoform X1

See identical proteins and their annotated locations for XP_006525882.1

Conserved Domains (2) summary

COG5236
Location:565 → 744

COG5236; Uncharacterized conserved protein, contains RING Zn-finger [General function prediction only]

sd00017
Location:416 → 436

ZF_C2H2; C2H2 Zn finger [structural motif]
XM_006525820.5 → XP_006525883.1 zinc finger protein 521 isoform X1

See identical proteins and their annotated locations for XP_006525883.1

Conserved Domains (2) summary

COG5236
Location:565 → 744

COG5236; Uncharacterized conserved protein, contains RING Zn-finger [General function prediction only]

sd00017
Location:416 → 436

ZF_C2H2; C2H2 Zn finger [structural motif]