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    hmp nitric oxide dioxygenase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947018, updated on 10-Oct-2024

    Summary

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    Official Symbol
    hmp
    Official Full Name
    nitric oxide dioxygenase
    Primary source
    ECOCYC:EG10456
    Locus tag
    b2552
    See related
    ASAP:ABE-0008396
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2549; fsrB; hmpA
    Summary
    Upregulated by GSNO in defined medium (aerobic) as part of the NsrR regulon. hmp mutants are defective in macrophage survival and swarming motility (Stevanin, 2007). [More information is available at EcoGene: EG10456].
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    Genomic context

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    Sequence:
    NC_000913.3 (2685835..2687025)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative DNA-binding transcriptional regulator, NAGC-like Neighboring gene serine hydroxymethyltransferase Neighboring gene nitrogen regulatory protein PII-1 Neighboring gene DNA-binding transcriptional activator GlrR Neighboring gene outer membrane lipoprotein QseG

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Roles of RpoS (sigmaS), IHF and ppGpp in the expression of the hmp gene encoding the flavohemoglobin (Hmp) of Escherichia coli K-12. Membrillo-Hernandez J, et al. Mol Gen Genet, 1997 May 20. PMID 9197421
    2. Novel hemoglobins to enhance microaerobic growth and substrate utilization in Escherichia coli. Bollinger CJ, et al. Biotechnol Prog, 2001 Sep-Oct. PMID 11587567
    3. Flavohemoglobin denitrosylase catalyzes the reaction of a nitroxyl equivalent with molecular oxygen. Hausladen A, et al. Proc Natl Acad Sci U S A, 2001 Aug 28. PMID 11517313, Free PMC Article
    4. Escherichia coli flavohaemoglobin (Hmp) with equistoichiometric FAD and haem contents has a low affinity for dioxygen in the absence or presence of nitric oxide. Mills CE, et al. Biochem J, 2001 Jan 15. PMID 11139382, Free PMC Article
    5. Flavohemoglobin Hmp affords inducible protection for Escherichia coli respiration, catalyzed by cytochromes bo' or bd, from nitric oxide. Stevanin TM, et al. J Biol Chem, 2000 Nov 17. PMID 10915782

    See all (55) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. comparison of expression of genes involved in folate metabolism and the hmp gene in strains with intact csgD gene and with deletion in csgD; expression of genes for methylene tetrahydrofolate synthesis and formyltetrahydrofolate recycling was higher in cells with CsgD than those without; expression of hmp was lower in the presence of CsgD
      Title: The influence of CsgD on the expression of genes of folate metabolism and hmp in Escherichia coli K-12.
    2. This study demonstrates, for the first time, that Hmp contributes to the protection of uropathogenic Escherichia coli against nitric oxide-mediated toxicity in vitro.
      Title: Role of flavohemoglobin in combating nitrosative stress in uropathogenic Escherichia coli--implications for urinary tract infection.
    3. HMP catalyzes oxidation of NO to NO(3)(-) (NO-dioxygenase activity) in vivo & in vitro in presence of O(2) & NADH. It also catalyzes reduction of several alkylhydroperoxide substrates into their corresponding alcohols using NADH as electron donor[review]
      Title: Flavohemoglobin: structure and reactivity.
    4. N-terminus verified by Edman degradation on complete protein
      Title: Nitric oxide dioxygenase: an enzymic function for flavohemoglobin.
    5. The lack of swarming motility of the hmp mutant and its aflagellate state suggest that Hmp synthesis is a metabolic burden in the absence of NO-related stresses.
      Title: The hmp gene encoding the NO-inducible flavohaemoglobin in Escherichia coli confers a protective advantage in resisting killing within macrophages, but not in vitro: links with swarming motility.
    6. produces superoxide; during prolonged oxidation of NADH, the spectral signals of Hmp decayed and iron was released in a process prevented by superoxide dismutase or catalase
      Title: Escherichia coli Hmp, an "oxygen-binding flavohaemoprotein", produces superoxide anion and self-destructs.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables FAD binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables FAD binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables FAD binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables fatty acid binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables heme binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables heme binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydroperoxide reductase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nitric oxide dioxygenase NAD(P)H activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables nitric oxide dioxygenase NAD(P)H activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables nitric oxide dioxygenase NAD(P)H activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nitric oxide dioxygenase NAD(P)H activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables oxidoreductase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxygen binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxygen carrier activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in cellular response to nitrosative stress IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in nitric oxide catabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in oxygen transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in response to nitrosative stress IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within response to nitrosative stress IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within response to nitrosative stress IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in response to toxic substance IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    nitric oxide dioxygenase
    NP_417047.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2685835..2687025
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417047.1 nitric oxide dioxygenase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417047.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A6N8NG14, A0AAQ2RBE7
      Conserved Domains (1) summary
      PRK13289
      Location:1 → 396
      PRK13289; NO-inducible flavohemoprotein
    Nucleotide Protein
    Heading Accession and Version

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