puc puckered [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: pucprovided by FlyBase
Official Full Name: puckeredprovided by FlyBase
Primary source: FLYBASE:FBgn0243512
Locus tag: Dmel_CG7850
See related: AllianceGenome:FB:FBgn0243512
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: 0238/03; 1351/08; CG7850; Dmel\CG7850; dPuc; hrt; JNK-DSP; l(3)84Eh; l(3)A251.1; l(3)j4E1; Puc; PUC; PUC/MKP; vco
Summary: Enables JUN kinase phosphatase activity. Involved in several processes, including eggshell chorion assembly; morphogenesis of an epithelium; and negative regulation of signal transduction. Located in Golgi apparatus and endoplasmic reticulum. Is expressed in several structures, including epithelium; larval imaginal tissue; longitudinal visceral muscle primordium; male reproductive system; and photoreceptor cell. Orthologous to several human genes including DUSP10 (dual specificity phosphatase 10). [provided by Alliance of Genome Resources, Apr 2022]
Orthologs: all
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Genomic context

Location:: 84E12-84E13; 3-48 cM

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3R	NT_033777.3 (8105332..8122294)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3R	NT_033777.2 (3931054..3948016)

Chromosome 3R - NT_033777.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Dissection of the Regulatory Elements of the Complex Expression Pattern of Puckered, a Dual-Specificity JNK Phosphatase.
Title: Dissection of the Regulatory Elements of the Complex Expression Pattern of Puckered, a Dual-Specificity JNK Phosphatase.
these results suggest a possible functional role of Puckered phosphorylation by MAPKs.
Title: The Drosophila DUSP puckered is phosphorylated by JNK and p38 in response to arsenite-induced oxidative stress.
Pacman genetically interacts with puckered, a phosphatase that negatively regulates the JNK signalling pathway.
Title: The 5'-3' exoribonuclease pacman is required for epithelial sheet sealing in Drosophila and genetically interacts with the phosphatase puckered.
Puc is a key anti-apoptotic factor that prevents apoptosis in epithelial cells by restraining basal JNK signaling
Title: Puckered, a Drosophila MAPK phosphatase, ensures cell viability by antagonizing JNK-induced apoptosis.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables JUN kinase phosphatase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables MAP kinase tyrosine phosphatase activity	IBA Inferred from Biological aspect of Ancestor more info
enables MAP kinase tyrosine/serine/threonine phosphatase activity	IBA Inferred from Biological aspect of Ancestor more info
enables phosphoprotein phosphatase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein tyrosine/threonine phosphatase activity	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in JNK cascade	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in actin cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to oxidative stress	IDA Inferred from Direct Assay more info	PubMed
involved_in chitin-based embryonic cuticle biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chorion micropyle formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in compound eye development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in determination of adult lifespan	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in determination of digestive tract left/right asymmetry	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dorsal appendage formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dorsal closure	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in embryonic anterior midgut (ectodermal) morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in epidermis development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in follicle cell of egg chamber development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in imaginal disc eversion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in imaginal disc fusion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in imaginal disc fusion, thorax closure	IGI Inferred from Genetic Interaction more info	PubMed
involved_in imaginal disc-derived male genitalia morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in imaginal disc-derived male genitalia morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of MAPK cascade	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of peptidoglycan recognition protein signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of negative regulation of programmed cell death	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of stress-activated protein kinase signaling cascade	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of tumor necrosis factor-mediated signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of border follicle cell migration	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of border follicle cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of immune response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of G2/M transition of mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to oxidative stress	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in wound healing	IEP Inferred from Expression Pattern more info	PubMed
involved_in wound healing	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: puckered

Names: CG7850-PA; CG7850-PB; Jun N-terminal kinase dual-specificity phosphatase; hearty; lethal (3) j4E1; puc-PA; puc-PB; velcro

NP_001287223.1

EC 3.1.3.-

NP_524273.1

EC 3.1.3.-

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033777.3 Reference assembly

Range

8105332..8122294

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_079549.3 → NP_524273.1 puckered, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_524273.1

Status: REVIEWED

UniProtKB/TrEMBL

O46122, Q9VHV8

Related

FBpp0081288

Conserved Domains (2) summary

COG2453
Location:193 → 272

CDC14; Protein-tyrosine phosphatase [Signal transduction mechanisms]

cd00127
Location:133 → 267

DSPc; Dual specificity phosphatases (DSP); Ser/Thr and Tyr protein phosphatases. Structurally similar to tyrosine-specific phosphatases but with a shallower active site cleft and a distinctive active site signature motif, HCxxGxxR. Characterized as VHR- or ...
NM_001300294.1 → NP_001287223.1 puckered, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001287223.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4LHX7

Conserved Domains (2) summary

COG2453
Location:86 → 165

CDC14; Protein-tyrosine phosphatase [Signal transduction mechanisms]

cd00127
Location:26 → 160

DSPc; Dual specificity phosphatases (DSP); Ser/Thr and Tyr protein phosphatases. Structurally similar to tyrosine-specific phosphatases but with a shallower active site cleft and a distinctive active site signature motif, HCxxGxxR. Characterized as VHR- or ...