birA DNA-binding transcriptional repressor/biotin-[acetyl-CoA-carboxylase] ligase BirA [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: birA
Official Full Name: DNA-binding transcriptional repressor/biotin-[acetyl-CoA-carboxylase] ligase BirA
Primary source: ECOCYC:EG10123
Locus tag: b3973
See related: ASAP:ABE-0013001
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: bioR; dhbB; ECK3965
Summary: Biotinyl-5'-AMP is the co-repressor and is also synthesized by BirA (Cronan, 1988). [More information is available at EcoGene: EG10123]. BirA is a bifunctional protein that exhibits biotin ligase activity and also acts as the DNA binding transcriptional repressor of the biotin operon . [More information is available at EcoCyc: EG10123].
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Genomic context

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Sequence:: NC_000913.3 (4173082..4174047)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A conserved regulatory mechanism in bifunctional biotin protein ligases BirA has been demonstrated for three bacterial strains.
Title: A conserved regulatory mechanism in bifunctional biotin protein ligases.
BirA signals to the global regulator Fur, which in turn activates LEE (locus of enterocyte effacement) genes to promote EHEC adherence in the low-biotin large intestine.
Title: Enterohemorrhagic Escherichia coli senses low biotin status in the large intestine for colonization and infection.
work demonstrates that variable surface loops evolved to support BirA function in the homodimerization required for transcription repression by complementing the constant loops, which are constrained because of their essential role in posttranslational biotin addition
Title: Functional versatility of a single protein surface in two protein:protein interactions.
BirA function entails extensive interactions among the three domains of the protein and show that normal ligase activity does not ensure normal DNA binding.
Title: Altered regulation of Escherichia coli biotin biosynthesis in BirA superrepressor mutant strains.
energetic and structural underpinnings of the dual specificity of protein BirA as a homodimer or as a heterodimer with acetyl coenzyme A carboxylase
Title: Thermodynamic and structural investigation of bispecificity in protein-protein interactions.
BirA ligase biotinylates K4, K9, K18 and K23 in histone H3.
Title: Prokaryotic BirA ligase biotinylates K4, K9, K18 and K23 in histone H3.
Results indicate that the hydrophobic cluster is critical to the ligand-induced disorder-to-order transition in birA and that this transition is integral to the allosteric response in the biotin repressor.
Title: Nucleation of an allosteric response via ligand-induced loop folding.
Results describe the 2.8A resolution crystal structure of BirA in complex with the co-repressor analog biotinol-5'-AMP.
Title: Co-repressor induced order and biotin repressor dimerization: a case for divergent followed by convergent evolution.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables biotin binding	IDA Inferred from Direct Assay more info	PubMed
enables biotin binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables biotin--[biotin carboxyl-carrier protein] ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables biotin--[biotin carboxyl-carrier protein] ligase activity	IDA Inferred from Direct Assay more info	PubMed
enables biotin--[biotin carboxyl-carrier protein] ligase activity	IEA Inferred from Electronic Annotation more info
enables ligase activity	IEA Inferred from Electronic Annotation more info
enables nucleic acid binding	EXP Inferred from Experiment more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables transcription cis-regulatory region binding	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within biotin biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in biotin metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein modification process	IEA Inferred from Electronic Annotation more info
involved_in regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
part_of transcription repressor complex	IDA Inferred from Direct Assay more info	PubMed
part_of transcription repressor complex	IMP Inferred from Mutant Phenotype more info	PubMed

General protein information

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Preferred Names: DNA-binding transcriptional repressor/biotin-[acetyl-CoA-carboxylase] ligase BirA

NP_418404.1

EC 6.3.4.15

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

4173082..4174047

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_418404.1 DNA-binding transcriptional repressor/biotin-[acetyl-CoA-carboxylase] ligase BirA [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_418404.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A8S7EPH2

Conserved Domains (1) summary

PRK11886
Location:2 → 320

PRK11886; bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA