map methionine aminopeptidase [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: map
Official Full Name: methionine aminopeptidase
Primary source: ECOCYC:EG10570
Locus tag: b0168
See related: ASAP:ABE-0000571
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK0166
Summary: All known proteins in Escherichia coli use N-formyl methionine as the first amino acid in a peptide chain. [More information is available at EcoCyc: EG10570].
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Genomic context

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Sequence:: NC_000913.3 (188712..189506, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Structural insights into the interplay of protein biogenesis factors with the 70S ribosome.
Title: Structural insights into the interplay of protein biogenesis factors with the 70S ribosome.
These data suggest that map expression contributes to the maintenance of enteropathogenic Escherichia coli colonization.
Title: Expression of enteropathogenic Escherichia coli map is significantly different than that of other type III secreted effectors in vivo.
Methionine aminopeptidase associates with ribosomes via a charged loop crucial for nascent-chain processing & cell viability. It competes with peptide deformylase for binding sites at the ribosomal tunnel exit
Title: Dynamic enzyme docking to the ribosome coordinates N-terminal processing with polypeptide folding.
Data show that Europium ion (Eu(3+)) binds to methionine aminopeptidase and binding results in the activation of the enzyme.
Title: Probing the metal ion selectivity in methionine aminopeptidase via changes in the luminescence properties of the enzyme bound europium ion.
N-terminus verified by Edman degradation on mature peptide
Title: Processing of the initiation methionine from proteins: properties of the Escherichia coli methionine aminopeptidase and its gene structure.
obtained a crystal structure for a complex of Escherichia coli methionine aminopeptidase with norleucine phosphonate, a transition-state analog, and only a single Mn(II) ion bound at the active site
Title: Structural basis of catalysis by monometalated methionine aminopeptidase.

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General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables aminopeptidase activity	IEA Inferred from Electronic Annotation more info
enables ferrous iron binding	IDA Inferred from Direct Assay more info	PubMed
enables initiator methionyl aminopeptidase activity	IDA Inferred from Direct Assay more info	PubMed
enables initiator methionyl aminopeptidase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables metalloaminopeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables metalloaminopeptidase activity	IDA Inferred from Direct Assay more info	PubMed
enables metalloaminopeptidase activity	IEA Inferred from Electronic Annotation more info
enables metalloexopeptidase activity	IEA Inferred from Electronic Annotation more info
enables transition metal ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in proteolysis	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: methionine aminopeptidase

NP_414710.1

EC 3.4.11.18

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

188712..189506 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_414710.1 methionine aminopeptidase [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_414710.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A7A6ZUW8

Conserved Domains (1) summary

PRK05716
Location:1 → 254

PRK05716; methionine aminopeptidase; Validated