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    uidA beta-glucuronidase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 946149, updated on 19-Sep-2024

    Summary

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    Official Symbol
    uidA
    Official Full Name
    beta-glucuronidase
    Primary source
    ECOCYC:EG11055
    Locus tag
    b1617
    See related
    ASAP:ABE-0005410
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK1612; gurA; gusA
    Summary
    UidA has an Ig-like domain: pfam PF00703 aligns to residues 182-272. ebgA, uidA and lacZ are paralogs. [More information is available at EcoGene: EG11055]. D-glucuronidase catalyzes the cleavage of a wide variety of -glucuronides . -D-glucuronidase and |FRAME: [More information is available at EcoCyc: EG11055].
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    Genomic context

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    Sequence:
    NC_000913.3 (1694260..1696071, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene DUF945 domain-containing protein YdgA Neighboring gene outer membrane porin family protein UidC Neighboring gene glucuronide:H(+) symporter Neighboring gene DNA-binding transcriptional repressor UidR Neighboring gene 7-alpha-hydroxysteroid dehydrogenase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. [On the properties and the biosynthesis of beta-glucuronidase of Escherichia coli K 12]. Didier-Fichet ML, et al. C R Acad Hebd Seances Acad Sci D, 1968 May 6. PMID 4970455
    2. A comparison of directed evolution approaches using the beta-glucuronidase model system. Rowe LA, et al. J Mol Biol, 2003 Sep 26. PMID 12972256
    3. Flow cytometric detection of beta-D-glucuronidase gene in wild-type bacterial cells using in-situ PCR. Sachidanandham R, et al. Biotechnol Bioeng, 2003 Apr 20. PMID 12584754
    4. Drug-Encoded Biomarkers for Monitoring Biological Therapies. Tsoneva D, et al. PLoS One, 2015. PMID 26348361, Free PMC Article
    5. Expression and purification of Escherichia coli beta-glucuronidase. Aich S, et al. Protein Expr Purif, 2001 Jun. PMID 11388802

    See all (40) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. We found GusA to be the most reliable biomarker for monitoring oncolytic virotherapy among the three tested markers
      Title: Drug-Encoded Biomarkers for Monitoring Biological Therapies.
    2. there is not only an active dimer of the E. coli form of beta-glucuronidase, but also several larger active complexes, including an octomer and a 16-mer
      Title: Native Electrophoresis-Coupled Activity Assays Reveal Catalytically-Active Protein Aggregates of Escherichia coli β-Glucuronidase.
    3. we performed in vitro directed evolution & obtained a mutant GUS that was highly thermostable. mutation & reversion approaches identified 6 key AAs (Q493R, T509A, M532T, N550S, G559S and N566S) that are responsible for the improved thermostability
      Title: Concurrent mutations in six amino acids in beta-glucuronidase improve its thermostability.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables beta-glucuronidase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables beta-glucuronidase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables beta-glucuronidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables carbohydrate binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables hydrolase activity, acting on glycosyl bonds IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity, hydrolyzing O-glycosyl compounds IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in carbohydrate metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of protein-containing complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    beta-glucuronidase
    NP_416134.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1694260..1696071 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_416134.1 beta-glucuronidase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_416134.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8I0CCI6
      Conserved Domains (1) summary
      PRK10150
      Location:1 → 600
      PRK10150; beta-D-glucuronidase; Provisional
    Nucleotide Protein
    Heading Accession and Version

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