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    inhA NADH-dependent enoyl-[ACP] reductase [ Mycobacterium tuberculosis H37Rv ]

    Gene ID: 886523, updated on 6-Oct-2023

    Summary

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    Gene symbol
    inhA
    Gene description
    NADH-dependent enoyl-[ACP] reductase
    Locus tag
    Rv1484
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Mycobacterium tuberculosis H37Rv (strain: H37Rv, type-material: type strain of Mycobacterium tuberculosis)
    Lineage
    Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex
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    Genomic context

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    Sequence:
    NC_000962.3 (1674202..1675011)

    NC_000962.3Genomic Context describing neighboring genes Neighboring gene hypothetical protein Neighboring gene 3-oxoacyl-ACP reductase FabG Neighboring gene ferrochelatase Neighboring gene hypothetical protein Neighboring gene hypothetical protein

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000962.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. In Vitro Antimycobacterial Activity and Physicochemical Characterization of Diaryl Ether Triclosan Analogues as Potential InhA Reductase Inhibitors. Ibrahim TS, et al. Molecules, 2020 Jul 8. PMID 32650556, Free PMC Article
    2. Transfer of a point mutation in Mycobacterium tuberculosis inhA resolves the target of isoniazid. Vilchèze C, et al. Nat Med, 2006 Sep. PMID 16906155
    3. Evidence from Raman spectroscopy that InhA, the mycobacterial enoyl reductase, modulates the conformation of the NADH cofactor to promote catalysis. Bell AF, et al. J Am Chem Soc, 2007 May 23. PMID 17472376
    4. Crystallographic studies on the binding of isonicotinyl-NAD adduct to wild-type and isoniazid resistant 2-trans-enoyl-ACP (CoA) reductase from Mycobacterium tuberculosis. Dias MV, et al. J Struct Biol, 2007 Sep. PMID 17588773
    5. Phosphorylation of enoyl-acyl carrier protein reductase InhA impacts mycobacterial growth and survival. Khan S, et al. J Biol Chem, 2010 Nov 26. PMID 20864541, Free PMC Article

    See all (34) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Pattern of InhA and KatG mutations in isoniazid mono resistant Mycobacterium tuberculosis isolates from Odisha.
      Title: Pattern of InhA and KatG mutations in isoniazid mono resistant Mycobacterium tuberculosis isolates from Odisha.
    2. In Vitro Antimycobacterial Activity and Physicochemical Characterization of Diaryl Ether Triclosan Analogues as Potential InhA Reductase Inhibitors.
      Title: In Vitro Antimycobacterial Activity and Physicochemical Characterization of Diaryl Ether Triclosan Analogues as Potential InhA Reductase Inhibitors.
    3. Identification of Novel Cytotoxic T Lymphocyte Epitopes of Drug- Resistance Related Protein InhA from Mycobacterium tuberculosis.
      Title: Identification of Novel Cytotoxic T Lymphocyte Epitopes of Drug- Resistance Related Protein InhA from Mycobacterium tuberculosis.
    4. This study provides valuable information on the diversity of polymorphisms in genes related to multidrug-resistant tuberculosis (including inhA), as well as the presence of new mutations not previously described; this information should be considered in the implementation of molecular diagnostic tests
      Title: rpoB, katG and inhA mutations in multi-drug resistant strains of Mycobacterium tuberculosis clinical isolates from southeast Mexico.
    5. Among the inhibin alpha promoter region mutations, 2(6.6%) of the thirty isoniazid resistant isolates had the most common C15T mutation in the promoter region.
      Title: Molecular characterisation of isoniazid resistant clinical isolates of Mycobacterium tuberculosis from Khyber Pakhtunkhwa, Pakistan.
    6. The formation of a covalent adduct during the InhA catalytic cycle and identified critical residues required for catalysis.
      Title: InhA, the enoyl-thioester reductase from Mycobacterium tuberculosis forms a covalent adduct during catalysis.
    7. It showed two molecules with comparatively higher affinity toward InhA as compared to potent molecule. It indicated the candidature of identified molecules to be further considered in anti-TB drug development pipeline.
      Title: Cell wall permeability assisted virtual screening to identify potential direct InhA inhibitors of Mycobacterium tuberculosis and their biological evaluation.
    8. Studied DNA-directed RNA polymerase subunit beta (rpoB), catalase-peroxidase (katG) and NADH-dependent enoyl-[ACP] reductase (inhA) mutations in clinical drug resistant Mycobacterium tuberculosis isolates.
      Title: Differences in rpoB, katG and inhA mutations between new and previously treated tuberculosis cases using the GenoType MTBDRplus assay.
    9. Study examine previously unstudied catalytic pocket residues through mutations in terms of their kinetics relating to NADH and DD-CoA substrate binding and turnover. The results demonstrate that mutation of NADH-binding pocket residues can have profound effects on DD-CoA binding and suggest that there are long range molecular interactions in the InhA protein.
      Title: Disruption of key NADH-binding pocket residues of the Mycobacterium tuberculosis InhA affects DD-CoA binding ability.
    10. Out of the 141 isolates studied, 3 (2.1%) were found carrying mutations in the katG gene that confer resistance to Isoniazid (INH). No mutations were detected in the inhA promoter region gene that confer weak INH resistance or in the rpoB gene that confer Rifampicin resistance.
      Title: The occurrence and frequency of genomic mutations that mediate Isoniazid and Rifampicin resistance in Mycobacterium tuberculosis isolates from untreated pulmonary Tuberculosis cases in urban Blantyre, Malawi.
    Submit: New GeneRIF Correction See all GeneRIFs (31)

    Pathways from PubChem

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    General gene information

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    Homology

    General protein information

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    Preferred Names
    NADH-dependent enoyl-[ACP] reductase
    NP_216000.1
    • NADH-dependent enoyl-ACP reductase

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000962.3 Reference assembly

      Range
      1674202..1675011
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_216000.1 NADH-dependent enoyl-[ACP] reductase [Mycobacterium tuberculosis H37Rv]

      See identical proteins and their annotated locations for NP_216000.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A4Y5N117, Q4TUY1
      Conserved Domains (1) summary
      PRK07889
      Location:1 → 269
      PRK07889; enoyl-[acyl-carrier-protein] reductase FabI
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    2NSD_A.1
    2NSD_B.1
    2PR2_A.1
    2X22_A.1
    2X22_B.1
    2X23_A.1
    2X23_B.1
    2X23_E.1
    2X23_G.1
    3OEW_A.1
    4BII_A.1
    4BII_B.1
    4BII_C.1
    4BII_D.1
    4BQP_A.1
    4BQP_B.1
    4BQP_C.1
    4BQP_D.1
    4BQP_E.1
    4BQP_F.1
    4BQR_A.1
    4BQR_B.1
    4BQR_C.1
    4BQR_D.1
    4D0S_A.1
    4D0S_B.1
    4D0S_C.1
    4D0S_D.1
    4QXM_A.1
    4QXM_C.1
    4QXM_E.1
    4QXM_G.1
    4TZK_A.1
    4TZT_A.1
    4U0J_A.1
    4U0K_A.1
    4UVD_A.1
    4UVE_A.1
    4UVG_A.1
    4UVH_A.1
    4UVH_B.1
    4UVH_C.1
    4UVH_D.1
    4UVI_A.1
    4UVI_B.1
    4UVI_C.1
    4UVI_D.1
    5G0S_A.1
    5G0S_B.1
    5G0S_C.1
    5G0S_D.1
    5G0T_A.1
    5G0T_B.1
    5G0T_C.1
    5G0T_D.1
    5G0U_A.1
    5G0U_B.1
    5G0U_C.1
    5G0U_D.1
    5G0V_A.1
    5G0V_B.1
    5G0V_C.1
    5G0V_D.1
    5G0W_A.1
    5G0W_B.1
    5G0W_C.1
    5G0W_D.1
    5JFO_A.1
    5JFO_B.1
    5JFO_C.1
    5JFO_D.1
    P9WGR1.1

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