RVS167 amphiphysin [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RVS167
Official Full Name: amphiphysin
Primary source: SGD:S000002796
Locus tag: YDR388W
See related: AllianceGenome:SGD:S000002796; FungiDB:YDR388W; VEuPathDB:YDR388W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables calmodulin binding activity; cytoskeletal protein binding activity; and protein homodimerization activity. Contributes to lipid binding activity. Involved in several processes, including actin cortical patch localization; mitotic actomyosin contractile ring assembly; and plasma membrane tubulation. Located in actin cortical patch; cellular bud tip; and mating projection tip. Part of Rvs161p-Rvs167p complex. Orthologous to human BIN3 (bridging integrator 3). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See RVS167 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (1250186..1251634)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Elevated PM tension, upon TORC2 inhibition, impinges on endocytosis at two different levels by (1) severing the bonds between the PM adaptor proteins Sla2 and Ent1 and the actin cytoskeleton and (2) hindering recruitment of Rvs167, an N-BAR-containing protein important for vesicle fission to endocytosis sites
Title: TORC2 controls endocytosis through plasma membrane tension.
Genetic, bioinformatics, biochemical and biophysical data show that attraction between this alpha1-conjugated ubiquitin and the HECT ubiquitin-binding patch pulls the alpha1-helix out of the interface, thereby promoting trimerization. Strikingly, trimerization renders the ubiquitin ligase inactive.
Title: Ubiquitylation-dependent oligomerization regulates activity of Nedd4 ligases.
deletion of RVS167 causes downregulation of de novo sphingolipid biosynthesis via Orm2
Title: Yeast lacking the amphiphysin family protein Rvs167 is sensitive to disruptions in sphingolipid levels.
reveal a conserved role for calmodulin in regulating the intrinsic membrane-sculpting activity of endocytic N-BAR domains, Calmodulin-binding mutants of Rvs167 exhibited defects in endocytic vesicle release
Title: Calmodulin Promotes N-BAR Domain-Mediated Membrane Constriction and Endocytosis.
59 of 131 in: PLoS One. 2013;8(2) (dup#16) Hof1 and Rvs167 have redundant roles in actomyosin ring function during cytokinesis
Title: Hof1 and Rvs167 have redundant roles in actomyosin ring function during cytokinesis in budding yeast.
Vps1 and Rvs167 function together to mediate scission during endocytosis in Saccharomyces cerevisiae.
Title: Yeast dynamin Vps1 and amphiphysin Rvs167 function together during endocytosis.
In gyp5Deltagyl1Delta cells, Rvs167p is not correctly localized to the small-bud tip.
Title: The RabGAP proteins Gyp5p and Gyl1p recruit the BAR domain protein Rvs167p for polarized exocytosis.
SUR4 does not remediate the steady-state actin cytoskeletal defects of rvs167 or rvs161 cells
Title: Characterizing the sphingolipid signaling pathway that remediates defects associated with loss of the yeast amphiphysin-like orthologs, Rvs161p and Rvs167p.
RVS161 and RVS167 have an identical set of 49 synthetic lethal interactions, revealing functions for the Rvs proteins in cell polarity, cell wall synthesis, and vesicle trafficking as well as a shared role in mating.
Title: Characterization of the yeast amphiphysins Rvs161p and Rvs167p reveals roles for the Rvs heterodimer in vivo.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables actin binding	IEA Inferred from Electronic Annotation more info
enables calmodulin binding	IDA Inferred from Direct Assay more info	PubMed
enables calmodulin binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables cytoskeletal protein binding	IPI Inferred from Physical Interaction more info	PubMed
contributes_to lipid binding	IBA Inferred from Biological aspect of Ancestor more info
contributes_to lipid binding	IDA Inferred from Direct Assay more info	PubMed
contributes_to lipid binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein homodimerization activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in actin cortical patch localization	IBA Inferred from Biological aspect of Ancestor more info
involved_in actin cortical patch localization	IEA Inferred from Electronic Annotation more info
involved_in actin cortical patch localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in endocytosis	IEA Inferred from Electronic Annotation more info
involved_in endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in lipid tube assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in mitotic actomyosin contractile ring assembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitotic actomyosin contractile ring assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in plasma membrane tubulation	IBA Inferred from Biological aspect of Ancestor more info
involved_in plasma membrane tubulation	IDA Inferred from Direct Assay more info	PubMed
involved_in plasma membrane tubulation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to cell division site	IGI Inferred from Genetic Interaction more info	PubMed
involved_in vesicle-mediated transport	IPI Inferred from Physical Interaction more info	PubMed

Component	Evidence Code	Pubs
part_of Rvs161p-Rvs167p complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Rvs161p-Rvs167p complex	IPI Inferred from Physical Interaction more info	PubMed
is_active_in actin cortical patch	IBA Inferred from Biological aspect of Ancestor more info
located_in actin cortical patch	IDA Inferred from Direct Assay more info	PubMed
is_active_in actin cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
located_in cellular bud tip	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in cytosol	HDA more info	PubMed
located_in mating projection tip	HDA more info	PubMed
is_active_in mating projection tip	IBA Inferred from Biological aspect of Ancestor more info
located_in mating projection tip	IDA Inferred from Direct Assay more info	PubMed
is_active_in medial cortex	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: amphiphysin

NP_010676.1

Calmodulin-binding actin-associated protein; roles in endocytic membrane tabulation and constriction, and exocytosis; N-BAR domain protein that interacts with Rvs161p to regulate actin cytoskeleton, endocytosis, and viability following starvation or osmotic stress; recruited to bud tips by Gyl1p and Gyp5p during polarized growth; homolog of mammalian amphiphysin; necessary for efficient trafficking of CoQ6 to mitochondria

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

1250186..1251634

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180696.1 → NP_010676.1 TPA: amphiphysin [Saccharomyces cerevisiae S288C]

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VT22, P39743

UniProtKB/TrEMBL

A6ZYY9, B3LFR2, C7GSD4, C8Z5V2, G2WBC8

Conserved Domains (2) summary

cd07599
Location:29 → 245

BAR_Rvs167p; The Bin/Amphiphysin/Rvs (BAR) domain of Saccharomyces cerevisiae Reduced viability upon starvation protein 167 and similar proteins

cl17036
Location:425 → 478

SH3; Src Homology 3 domain superfamily