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    TIR1 F-box/RNI-like superfamily protein [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 825473, updated on 18-Sep-2024

    Summary

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    Official Symbol
    TIR1
    Official Full Name
    F-box/RNI-like superfamily protein
    Primary source
    TAIR:AT3G62980
    Locus tag
    AT3G62980
    See related
    Araport:AT3G62980
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    AtTIR1; TRANSPORT INHIBITOR RESPONSE 1
    Summary
    Encodes an auxin receptor that mediates auxin-regulated transcription. It contains leucine-rich repeats and an F-box and interacts with ASK1, ASK2 and AtCUL1 to form SCF-TIR1, an SCF ubiquitin ligase complex. Related to yeast Grr1p and human SKP2 proteins, involved in ubiquitin-mediated processes. Required for normal response to auxin and repressed in response to flagellin. As part of the SCF complex and in the presence of auxin, TIR1 interacts with Aux/IAA transcriptional repressor proteins and mediates their degradation. Mutations in TIR1 block auxin stimulation of flavonoid synthesis.
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    Genomic context

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    See TIR1 in Genome Data Viewer
    Location:
    chromosome: 3
    Exon count:
    3
    Sequence:
    Chromosome: 3; NC_003074.8 (23273065..23276630, complement)

    Chromosome 3 - NC_003074.8Genomic Context describing neighboring genes Neighboring gene Thioredoxin superfamily protein Neighboring gene zinc finger (C3HC4-type RING finger) family protein Neighboring gene myelin transcription factor-like protein Neighboring gene NPL4-like protein 1

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003074.8

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Regulation of auxin response by miR393-targeted transport inhibitor response protein 1 is involved in normal development in Arabidopsis. Chen ZH, et al. Plant Mol Biol, 2011 Dec. PMID 22042293
    2. The TIR1 protein of Arabidopsis functions in auxin response and is related to human SKP2 and yeast grr1p. Ruegger M, et al. Genes Dev, 1998 Jan 15. PMID 9436980, Free PMC Article
    3. Small-molecule agonists and antagonists of F-box protein-substrate interactions in auxin perception and signaling. Hayashi K, et al. Proc Natl Acad Sci U S A, 2008 Apr 8. PMID 18391211, Free PMC Article
    4. Auxin activates the plasma membrane H+-ATPase by phosphorylation during hypocotyl elongation in Arabidopsis. Takahashi K, et al. Plant Physiol, 2012 Jun. PMID 22492846, Free PMC Article
    5. Genetic analysis of the Arabidopsis TIR1/AFB auxin receptors reveals both overlapping and specialized functions. Prigge MJ, et al. Elife, 2020 Feb 18. PMID 32067636, Free PMC Article

    See all (63) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. FERONIA-mediated TIR1/AFB2 oxidation stimulates auxin signaling in Arabidopsis.
      Title: FERONIA-mediated TIR1/AFB2 oxidation stimulates auxin signaling in Arabidopsis.
    2. TIR signal promotes interactions between lipase-like proteins and ADR1-L1 receptor and ADR1-L1 oligomerization.
      Title: TIR signal promotes interactions between lipase-like proteins and ADR1-L1 receptor and ADR1-L1 oligomerization.
    3. Genetic analysis of the Arabidopsis TIR1/AFB auxin receptors reveals both overlapping and specialized functions.
      Title: Genetic analysis of the Arabidopsis TIR1/AFB auxin receptors reveals both overlapping and specialized functions.
    4. Control of Adventitious Rooting by TIR1/AFB2-Aux/IAA-Dependent Auxin Signaling in Arabidopsis
      Title: A Molecular Framework for the Control of Adventitious Rooting by TIR1/AFB2-Aux/IAA-Dependent Auxin Signaling in Arabidopsis.
    5. In vitro and in vivo protein-protein interaction assays show that NO enhances ASK1 binding to CUL1 and TIR1/AFB2, required for SCF(TIR1/AFB2) assembly.
      Title: Regulation of SCF(TIR1/AFBs) E3 ligase assembly by S-nitrosylation of Arabidopsis SKP1-like1 impacts on auxin signaling.
    6. The miR393-TIR1 molecular regulation pathway appears to be a component of the regulatory control over shoot regeneration from in vitro culture.
      Title: miR393 inhibits in vitro shoot regeneration in Arabidopsis thaliana via repressing TIR1.
    7. TIR1/AFB-Aux/IAA auxin perception mediates rapid cell wall acidification and growth of Arabidopsis hypocotyls.
      Title: TIR1/AFB-Aux/IAA auxin perception mediates rapid cell wall acidification and growth of Arabidopsis hypocotyls.
    8. HSP90 stabilizes auxin receptor TIR1 and ensures plasticity of auxin responses
      Title: HSP90 stabilizes auxin receptor TIR1 and ensures plasticity of auxin responses.
    9. Auxin signaling through TIR1/AFB2/AFB3 mediates feedback regulation of indole-3-acetic acid biosynthesis.
      Title: Auxin signaling through SCF(TIR1/AFBs) mediates feedback regulation of IAA biosynthesis.
    10. We propose oligomerization of TIR1 as a novel regulatory mechanism in the regulation of auxin-mediated plant patterning and development.
      Title: Oligomerization of SCFTIR1 Is Essential for Aux/IAA Degradation and Auxin Signaling in Arabidopsis.
    Submit: New GeneRIF Correction See all GeneRIFs (32)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    NM_116163.4

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables auxin binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables auxin binding IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    enables auxin receptor activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables inositol hexakisphosphate binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables ubiquitin-protein transferase activity IC
    Inferred by Curator
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in auxin-activated signaling pathway IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within cellular response to phosphate starvation IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within lateral root formation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within pollen maturation IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in protein ubiquitination IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within response to auxin IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within stamen development IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within ubiquitin-dependent protein catabolic process TAS
    Traceable Author Statement
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of SCF ubiquitin ligase complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm ISM
    Inferred from Sequence Model
    more info
    		  
    part_of ubiquitin ligase complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    F-box/RNI-like superfamily protein
    NP_567135.1
    • TRANSPORT INHIBITOR RESPONSE 1 (TIR1); CONTAINS InterPro DOMAIN/s: F-box domain, cyclin-like (InterPro:IPR001810), F-box domain, Skp2-like (InterPro:IPR022364); BEST Arabidopsis thaliana protein match is: GRR1-like protein 1 (TAIR:AT4G03190.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003074.8 Reference assembly

      Range
      23273065..23276630 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_116163.4 → NP_567135.1  F-box/RNI-like superfamily protein [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_567135.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      A5YZP2, B2CVU0, O24660, Q570C0
      UniProtKB/TrEMBL
      A0A178VA54, A0A5S9XNA6
      Conserved Domains (4) summary
      cd09293
      Location:116 → 224
      AMN1; Antagonist of mitotic exit network protein 1
      sd00034
      Location:291 → 315
      LRR_AMN1; leucine-rich repeat [structural motif]
      pfam18511
      Location:8 → 47
      F-box_5
      pfam18791
      Location:66 → 112
      Transp_inhibit; Transport inhibitor response 1 protein domain
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q570C0.2

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