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    CYP94B3 cytochrome P450, family 94, subfamily B, polypeptide 3 [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 824011, updated on 18-Sep-2024

    Summary

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    Official Symbol
    CYP94B3
    Official Full Name
    cytochrome P450, family 94, subfamily B, polypeptide 3
    Primary source
    TAIR:AT3G48520
    Locus tag
    AT3G48520
    See related
    Araport:AT3G48520
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    cytochrome P450; family 94; polypeptide 3; subfamily B
    Summary
    CYP94B3 is a jasmonoyl-isoleucine-12-hydroxylase that catalyzes the formation of 12-OH-JA-Ile from JA-Ile. By reducing the levels of this the biologically active phytohormone, CYP94B3 attenuates the jasmonic acid signaling cascade. CYP94B3 transcript levels rise in response to wounding.
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    Genomic context

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    See CYP94B3 in Genome Data Viewer
    Location:
    chromosome: 3
    Exon count:
    1
    Sequence:
    Chromosome: 3; NC_003074.8 (17974853..17976774, complement)

    Chromosome 3 - NC_003074.8Genomic Context describing neighboring genes Neighboring gene AtIII18x5-like protein Neighboring gene tRNA-Tyr Neighboring gene ncRNA Neighboring gene pseudo

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003074.8

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. CYP94B3 activity against jasmonic acid amino acid conjugates and the elucidation of 12-O-β-glucopyranosyl-jasmonoyl-L-isoleucine as an additional metabolite. Kitaoka N, et al. Phytochemistry, 2014 Mar. PMID 24467969
    2. Arabidopsis CYP94B3 encodes jasmonyl-L-isoleucine 12-hydroxylase, a key enzyme in the oxidative catabolism of jasmonate. Kitaoka N, et al. Plant Cell Physiol, 2011 Oct. PMID 21849397
    3. WRKY9 transcription factor regulates cytochrome P450 genes CYP94B3 and CYP86B1, leading to increased root suberin and salt tolerance in Arabidopsis. Krishnamurthy P, et al. Physiol Plant, 2021 Jul. PMID 33619745
    4. Sequential oxidation of Jasmonoyl-Phenylalanine and Jasmonoyl-Isoleucine by multiple cytochrome P450 of the CYP94 family through newly identified aldehyde intermediates. Widemann E, et al. Phytochemistry, 2015 Sep. PMID 26164240
    5. CYP94-mediated jasmonoyl-isoleucine hormone oxidation shapes jasmonate profiles and attenuates defence responses to Botrytis cinerea infection. Aubert Y, et al. J Exp Bot, 2015 Jul. PMID 25903915, Free PMC Article

    See all (11) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. WRKY9 transcription factor regulates cytochrome P450 genes CYP94B3 and CYP86B1, leading to increased root suberin and salt tolerance in Arabidopsis.
      Title: WRKY9 transcription factor regulates cytochrome P450 genes CYP94B3 and CYP86B1, leading to increased root suberin and salt tolerance in Arabidopsis.
    2. we created the double and triple knock-out mutants of three CYP94 genes, CYP94B1, CYP94B3, and CYP94C1. The mutations blocked the oxidation steps and caused JA-Ile to accumulate 3-4-fold the WT levels in the wounded leaves.
      Title: Mutations in jasmonoyl-L-isoleucine-12-hydroxylases suppress multiple JA-dependent wound responses in Arabidopsis thaliana.
    3. CYP94B3 and CYP94C1 are responsible for the production of oxidized derivatives of Jasmonoyl-Phenylalanine and Jasmonoyl-Isoleucine in wounded plants.
      Title: Sequential oxidation of Jasmonoyl-Phenylalanine and Jasmonoyl-Isoleucine by multiple cytochrome P450 of the CYP94 family through newly identified aldehyde intermediates.
    4. These results highlight the dual function of CYP94B3/C1 in antimicrobial defence.
      Title: CYP94-mediated jasmonoyl-isoleucine hormone oxidation shapes jasmonate profiles and attenuates defence responses to Botrytis cinerea infection.
    5. It was found that CYP94B3 also catalyzes the hydroxylation of jasmonoyl-L-valine and jasmonoyl-L-phenylalanine, and that these hydroxyl compounds accumulated after wounding and possessed lower activity than non-hydroxylated compounds.(cytohrome P-450 94B3)
      Title: CYP94B3 activity against jasmonic acid amino acid conjugates and the elucidation of 12-O-β-glucopyranosyl-jasmonoyl-L-isoleucine as an additional metabolite.
    6. These results identify CYP94B3/C1-mediated oxidation as a major catabolic route for turning over the JA-Ile hormone.
      Title: Cytochromes P450 CYP94C1 and CYP94B3 catalyze two successive oxidation steps of plant hormone Jasmonoyl-isoleucine for catabolic turnover.
    7. CYP94B3 plays a major regulatory role in controlling the level of jasmonyl-L-isoleucine in plants.
      Title: Arabidopsis CYP94B3 encodes jasmonyl-L-isoleucine 12-hydroxylase, a key enzyme in the oxidative catabolism of jasmonate.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    NM_114710.3

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables heme binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables iron ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables jasmonoyl-isoleucine-12-hydroxylase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within anther development IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within defense response to insect IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within fruit development IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within jasmonic acid metabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within pollen development IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within response to wounding IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within stigma development IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in endoplasmic reticulum membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in extracellular region ISM
    Inferred from Sequence Model
    more info
    		  

    General protein information

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    Preferred Names
    cytochrome P450, family 94, subfamily B, polypeptide 3
    NP_190421.1
    • ''cytochrome P450, family 94, subfamily B, polypeptide 3'' (CYP94B3); FUNCTIONS IN: electron carrier activity, monooxygenase activity, iron ion binding, oxygen binding, heme binding; INVOLVED IN: oxidation reduction; LOCATED IN: endomembrane system; EXPRESSED IN: 14 plant structures; EXPRESSED DURING: 7 growth stages; CONTAINS InterPro DOMAIN/s: Cytochrome P450 (InterPro:IPR001128), Cytochrome P450, E-class, group I (InterPro:IPR002401); BEST Arabidopsis thaliana protein match is: cytochrome P450, family 94, subfamily B, polypeptide 1 (TAIR:AT5G63450.1); Has 27655 Blast hits to 27536 proteins in 1435 species: Archae - 44; Bacteria - 2616; Metazoa - 10459; Fungi - 5693; Plants - 7745; Viruses - 3; Other Eukaryotes - 1095 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003074.8 Reference assembly

      Range
      17974853..17976774 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_114710.3 → NP_190421.1  cytochrome P450, family 94, subfamily B, polypeptide 3 [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_190421.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      Q9SMP5
      UniProtKB/TrEMBL
      A0A178V8H3, A0A5S9XJ92
      Conserved Domains (1) summary
      cl12078
      Location:46 → 501
      p450; Cytochrome P450
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9SMP5.1

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