Dlat dihydrolipoamide S-acetyltransferase [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Dlatprovided by RGD
Official Full Name: dihydrolipoamide S-acetyltransferaseprovided by RGD
Primary source: RGD:619859
See related: EnsemblRapid:ENSRNOG00000009994 AllianceGenome:RGD:619859
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables dihydrolipoyllysine-residue acetyltransferase activity. Involved in acetyl-CoA biosynthetic process from pyruvate. Located in mitochondrion. Part of pyruvate dehydrogenase complex. Human ortholog(s) of this gene implicated in pyruvate decarboxylase deficiency. Orthologous to human DLAT (dihydrolipoamide S-acetyltransferase). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Heart (RPKM 1086.2), Muscle (RPKM 713.9) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 8q23

Exon count:: 15

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	8	NC_086026.1 (59875537..59900947, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	8	NC_051343.1 (50979151..51004435, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	8	NC_005107.4 (55062549..55087832, complement)

Chromosome 8 - NC_086026.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH128680 (e-PCR)
- UniSTS:211988

RH132308 (e-PCR)
- UniSTS:215591

RH139711 (e-PCR)
- UniSTS:221795

RH132630 (e-PCR)
- UniSTS:215913

RH127531 (e-PCR)
- UniSTS:210841

RH138741 (e-PCR)
- UniSTS:220828

DLAT (e-PCR)
- UniSTS:527313

Dlat (e-PCR), detects polymorphism
- UniSTS:527313

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables acetyltransferase activity	ISO Inferred from Sequence Orthology more info
enables dihydrolipoyllysine-residue acetyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables dihydrolipoyllysine-residue acetyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables dihydrolipoyllysine-residue acetyltransferase activity	IEA Inferred from Electronic Annotation more info
enables dihydrolipoyllysine-residue acetyltransferase activity	ISO Inferred from Sequence Orthology more info
enables dihydrolipoyllysine-residue acetyltransferase activity	TAS Traceable Author Statement more info	PubMed
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables nucleotide binding	TAS Traceable Author Statement more info	PubMed
contributes_to pyruvate dehydrogenase (NAD+) activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in acetyl-CoA biosynthetic process from pyruvate	IBA Inferred from Biological aspect of Ancestor more info
involved_in acetyl-CoA biosynthetic process from pyruvate	IDA Inferred from Direct Assay more info	PubMed
involved_in acetyl-CoA biosynthetic process from pyruvate	IEA Inferred from Electronic Annotation more info
involved_in acetyl-CoA biosynthetic process from pyruvate	ISO Inferred from Sequence Orthology more info
involved_in glucose metabolic process	IEA Inferred from Electronic Annotation more info
involved_in tricarboxylic acid cycle	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
located_in intracellular membrane-bounded organelle	ISO Inferred from Sequence Orthology more info
located_in mitochondrial matrix	IEA Inferred from Electronic Annotation more info
is_active_in mitochondrial matrix	ISO Inferred from Sequence Orthology more info
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	ISO Inferred from Sequence Orthology more info
part_of pyruvate dehydrogenase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of pyruvate dehydrogenase complex	IDA Inferred from Direct Assay more info	PubMed
part_of pyruvate dehydrogenase complex	IEA Inferred from Electronic Annotation more info
part_of pyruvate dehydrogenase complex	ISO Inferred from Sequence Orthology more info
part_of pyruvate dehydrogenase complex	TAS Traceable Author Statement more info	PubMed

General protein information

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Preferred Names: dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial

Names: 70 kDa mitochondrial autoantigen of primary biliary cirrhosis; E2; PBC; PDC-E2; PDCE2; dihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydrogenase complex); dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; pyruvate dehydrogenase complex component E2

NP_112287.1

EC 2.3.1.12

XP_063122274.1

EC 2.3.1.12

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_031025.1 → NP_112287.1 dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial

See identical proteins and their annotated locations for NP_112287.1

Status: PROVISIONAL

Source sequence(s)

BC107440

UniProtKB/Swiss-Prot

P08461, Q3B7V7

UniProtKB/TrEMBL

A0A8I6B5J2, A6J4E7

Related

ENSRNOP00000103252.1, ENSRNOT00000148798.1

Conserved Domains (4) summary

cd06849
Location:209 → 283

lipoyl_domain; Lipoyl domain of the dihydrolipoyl acyltransferase component (E2) of 2-oxo acid dehydrogenases. 2-oxo acid dehydrogenase multienzyme complexes, like pyruvate dehydrogenase (PDH), 2-oxoglutarate dehydrogenase (OGDH) and branched-chain 2-oxo acid ...

TIGR01349
Location:211 → 632

PDHac_trf_mito; pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase, long form

pfam00198
Location:421 → 632

2-oxoacid_dh; 2-oxoacid dehydrogenases acyltransferase (catalytic domain)

pfam02817
Location:342 → 375

E3_binding; e3 binding domain